LOCUS       V005627                 7504 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005627
VERSION     V005627
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ji M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2015) Shanghai Advanced Research Institute, CAS,
            Haike Road 99, Shanghai 201210, China
REFERENCE   2  (bases 1 to 7504)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (16-DEC-2015) Shanghai Advanced Research Institute, CAS, Haike Road
            99, Shanghai 201210, China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             746..760
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             complement(1499..2356)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2357..2461)
                     /label="AmpR promoter"
     primer_bind     2935..2951
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2952..3129
                     /label="xylA promoter"
                     /note="xylA promoter"
                     /regulatory_class="promoter"
     CDS             3128..3841
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(3881..3897)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3905..3921)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3929..3959)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3974..3995)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5074..5721)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 gctggggact gaacgatnaa gcattgaang ttncgcgctg gaactgaagn ctggttctgn
       61 ggcanttang caggaagggt tntttactgc nccctaggag gggggatgga ngaataacgt
      121 tatatcttng aacntaaagg aaaangggag gtaaagggtt gtcgtagtag gttccttata
      181 aaanggagnt ntatcccatt cagggtaatg cgnaataatc gtntcncaga aaagatttat
      241 ttagccacca gaanggtaga ttangcgtga tgaacgggca ttcagaatac cccagtncan
      301 gtccaaaaaa ttaggccaaa ttataataaa gaattatatg aaatggctaa gtattctggt
      361 aaagatagtg attatttaat aaatcaaaaa gtctttgatg cattttataa atcacttaaa
      421 ggtaaacagg tattagttta ttcaggatta tttaaagagg ctaaaaagaa attaaaaaat
      481 ggggatttag attacttaaa agaaattgat ccaaccgaat atatctatca aattttttat
      541 atttggaaac aaaaagagta tttagctagt gaactttatg acttaacaga acaagaaaaa
      601 agagaaatta atcacaaaat gatagacgaa atcgaggaag aacaataaca aaatataagt
      661 gctaacagct gacctcccga taacaccatg tagttattgg gaggtcagct gttgaattat
      721 gcacgagtat tttaaaagtt attgtgatga cgacgataaa cgattatcaa aagtataatg
      781 ttaaaatgct ttattatact aacgttatat aaacattata ctttcgttat acaaatttta
      841 accctgttag gaactataaa aaatcatgaa aattttaatt tgcatgtaac tgggcagtgt
      901 cttaaaaaat cgacactgaa tttgctcaaa tttttgtttg tagaattaga atatatttat
      961 ttggctcata tttgcttttt aaaagcttct gtaggttttt aggcataaaa ctatatgatt
     1021 tacccctaaa tctttaaaat gccccttaaa attcaaaata aaggcattta aaatttaaat
     1081 atttcttgtg ataaagtttg ttaaaaagga gtggttttat gactgttatg tggttatcga
     1141 ttataggtat gtggttttgt attggaatgg cattttttgc tatcaaggtt attaaaaata
     1201 aaaattagac cacgcattta tgccgagaaa atttattgtg cgttgagaag aacccttaac
     1261 taaacttgca gacgaatgtc ggcatagcgt gagctattaa gccgaccatt cgacaagttt
     1321 tgggattgtt aagggttccg aggctcaacg tcaataaagc aattggaata aagaagcgaa
     1381 aaaggagaag tcggttcaga aaaagaagga tatggatctt cacctagatc cttttaaatt
     1441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2461 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2521 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     2581 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2641 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2701 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     2761 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     2821 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2881 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2941 cgacggccag tgaattctag aatctaatat tataactaaa ttttctaaaa aaaacattgg
     3001 aatagacatt tattttgtat atgatgaaat aaagttagtt tattggataa acaaactaac
     3061 tttattaagg tagttgatgg ataaacttgt tcacttaaat caacccggga acaaggagga
     3121 ataaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     3181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     3241 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     3301 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     3361 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     3421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     3481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     3541 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     3601 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     3661 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     3721 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     3781 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     3841 ataaatgtgt cgacctgcag ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     3901 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3961 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4021 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4081 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4141 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4201 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4261 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4321 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4441 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4681 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4741 gcgctctgct gaagccagtt accttcggaa aaagagttga tagctcttga tccggcaaac
     4801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4861 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4921 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatctgga
     4981 gctgtaatat aaaaaccttc ttcaactaac ggggcaggtt agtgacatta gaaaaccgac
     5041 tgtaaaaagt acagtcggca ttatctcata ttataaaagc cagtcattag gcctatctga
     5101 caattcctga atagagttca taaacaatcc tgcatgataa ccatcacaaa cagaatgatg
     5161 tacctgtaaa gatagcggta aatatattga attaccttta ttaatgaatt ttcctgctgt
     5221 aataatgggt agaaggtaat tactattatt attgatattt aagttaaacc cagtaaatga
     5281 agtccatgga ataatagaaa gagaaaaagc attttcaggt ataggtgttt tgggaaacaa
     5341 tttccccgaa ccattatatt tctctacatc agaaaggtat aaatcataaa actctttgaa
     5401 gtcattcttt acaggagtcc aaataccaga gaatgtttta gatacaccat caaaaattgt
     5461 ataaagtggc tctaacttat cccaataacc taactctccg tcgctattgt aaccagttct
     5521 aaaagctgta tttgagttta tcacccttgt cactaagaaa ataaatgcag ggtaaaattt
     5581 atatccttct tgttttatgt ttcggtataa aacactaata tcaatttctg tggttatact
     5641 aaaagtcgtt tgttggttca aataatgatt aaatatctct tttctcttcc aattgtctaa
     5701 atcaatttta ttaaagttca tttgatatgc ctcctaaatt tttatctaaa gtgaatttag
     5761 gaggcttact tgtctgcttt cttcattaga atcaatcctt ttttaaaagt caatattact
     5821 gtaacataaa tatatatttt aaaaatatcc cactttatcc aattttcgtt tgttgaacta
     5881 atgggtgctt tagttgaaga ataaagacca cattaaaaaa tgtggtcttt tgtgtttttt
     5941 taaaggattt gagcgtagcg aaaaatcctt ttctttctta tcttgataat aagggtaact
     6001 attgccggcg aggctagtta cccttaagtt attggtatga ctggttttaa gcgcaaaaaa
     6061 agttgctttt tcgtacctat taatgtatcg ttttaaatga ctagtaaaaa acatacatag
     6121 aaaggggaaa aagcaacttt ttttattgtc atagtttgtg aaaactaagt tgtttttatg
     6181 tgttataaca tggaaaagta tactgagaaa aaacaaagaa atcaagtatt tcagaaattt
     6241 attaaacgtc atattggtcc tgtctgtgct tggagaaaag ctagaaaaga gcttgggttt
     6301 atctttgatg atgcaatata ttaagcagcc cgagaaaaag gagtttatct ttttaacttt
     6361 gactacacct aatgtaatga gtgatgaatt agaaaatgaa ataaaacgtt ataataattc
     6421 ttttagaaaa cttataaaga gaaaaaaagt aggtagtgtt ataaagggat atgttcgtaa
     6481 gttagagatt acatataata aaaaaagaga tgattataat cctcattttc atgtgttaat
     6541 tgcagtaaat aaatcgtatt tcacagataa gagatattat attagccaac aagaatggtt
     6601 agatttatgg cgtgatgtaa cgggcatttc agaaataaca caagttcaag ttcaaaaaat
     6661 aagacaaaat aataataaag aattatatga aatggctaag tattctggta aagatagtga
     6721 ttatttaata aatcaaaaag tctttgatgc attttataaa tcacttaaag gtaaacaggt
     6781 attagtttat tcaggattat ttaaagaggc taaaaagaaa ttaaaaaatg gggatttaga
     6841 ttacttaaaa gaaattgatc caaccgaata tatctatcaa attttttata tttggaaaca
     6901 aaaagagtat ttagctagtg aactttatga cttaacagaa caagaaaaaa gagaaattaa
     6961 tcaccaaatg atagacgaaa tcgaggaaga ncataaccaa agagaatcaa atggatttag
     7021 ttgaagattg caatacattt ctgtcttttg tagctgataa aactttagaa aaacagaaat
     7081 tatataaagc taattcttgt aaaaatcgat tttgtcctgt ctgtgcttgg agaaaagcta
     7141 gaaaagatgc attgggttta tctttgatga tgcaatatat taagcagcaa gagaaaaagg
     7201 agtttatctt tttaactttg actacaccta atgtaatgag tgatgaatta gaaaatgaaa
     7261 taaaacgtta taataattct tttagaaaac ttataaagag aaaaaaagta ggtagtgtta
     7321 taaagggata tgttcgtaag ttagagatta catataataa aaaaagagat gattataatc
     7381 ctcattttca tgtgttaatt gcagtaaata aatcgtattt cacagataaa agatattata
     7441 ttagccaaca agaatggtta gatttatggc gtgatgtaac gggcatttca gaaataacac
     7501 aagt
//
