LOCUS       40924_23899       12655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB770, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Development of Gateway Binary Vectors, pGWBn70, for Ectopic 
            Expression of Dominant Transcription Activators in Higher Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12655)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2013) Agricultural Bioscience, Nagoya University, 
            Fro-Cho Chikusa-Ku, Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 12655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2013) Agricultural Bioscience, Nagoya University, Fro-Cho 
            Chikusa-Ku, Nagoya, Aichi 4648601, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1089..1155
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1170..1294
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1331..1361
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1415..2071
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2416..2718
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2762..2886)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2900..3133
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3155..3388
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      3419..3671
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3705..3721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3729..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3753..3783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3798..3819)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3855..4107)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4145..5440)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="AGO05991.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4145..5440)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5492..5671)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6177..6201)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6722..7510
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7759..8347
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8533..8673)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9017..9211)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9280..10350)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10782..11408)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gagttatcaa caagtttgta caaaaaagct gaacgagaaa
     1201 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1261 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1321 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1381 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1441 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1501 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1561 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1621 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1681 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1741 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1801 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1861 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1921 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1981 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2041 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2101 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2161 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2221 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2281 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2341 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2401 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2461 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     2521 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     2581 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     2641 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     2701 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     2761 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     2821 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     2881 agtggttgat aacagcatgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     2941 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     3001 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     3061 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     3121 cgagtacggt ggggaattcc cgaagcttaa agtcgccccc ccgaccgatg tcagcctggg
     3181 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     3241 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     3301 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     3361 tgcccttgga attgacgagt acggtgggta atcgcgagct tagagctcga atttccccga
     3421 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     3481 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     3541 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     3601 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     3661 gttactagat cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct
     3721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3781 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     3841 ttctattaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     3901 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     3961 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     4021 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     4081 actttattgc caaatgtttg aacgatcggg gaaattcgcc tcgagtgact tctctgattg
     4141 cagactattt ataccaacca gcaagaaagt gtcgaagtat gaagcagaag gcgcaggcta
     4201 gagcctggaa aacgagaaga tggatgcaaa gagacttttc tgatttagga ccaaacaacc
     4261 tcaagtaaac attgacgagt gtcccatctt ttgtgccggt cagtaatccg gtagcaggat
     4321 cataccttgg gagacgatgg cgcggacatt tcacaatccc agcaagctga ggaagcgata
     4381 atagaaggtt cagaacttga ggaagaaaga agatgaggag ggtttcactg aagtgaccca
     4441 gaataccaac aactgccata gtcattccgg cgaagactgt gtaagtgtct ccaacaaaaa
     4501 ctgaagaagg ataccaattg taagcaagca ttgccaagga tgtggccatc agcggttgag
     4561 taagaaatat cgagaaggca tgagcttggt gatactcggg atcaacagat gctccgattt
     4621 gcataacgtt gtgtatcagg atagcagcgg caataacaac tgtttgaccg atttcaaggc
     4681 cattcagacc agcgtggatg ttaatagagt ttgtacaaaa gacagcaagt agccccatat
     4741 ataacttata tatccttcct agattaagca cttccaagcc aatataagca acaagaggtt
     4801 ttggtataac aatagttgta tgaccagcat aagccatcaa aagtggaagg gtagcaaaag
     4861 atggcaggac aagttttact ctccaaggca catcaaggac atcatctaca aatccaagca
     4921 aaatcatgaa gcaaatagat gctagtgctg cattatactc cacaagccaa ttagaatctt
     4981 cagtgaagtt aaaatactga aatattatcg ccacgatcaa aaagacaata ccaacaacaa
     5041 tacccaatga ctctggcact ttaatatctc cttgaggcgt gcctcttttg ttgatatcaa
     5101 aaccaaacat gttacgtctc agaacgtatc tggcagtaac aggaatcatt ttgagagtga
     5161 cgaaaaaccc aaccaagcta agccccgcgt taattagaat cgatcgcttg agttcattat
     5221 caaccttgta gtgaaaacag agaaggtata gatagagcga acagagaaga gtagagatca
     5281 cgaagatcac tcctaatttc ggcggcgcga gacgaaattc ctcaccggaa gcagaaaccg
     5341 tctttcgggt catcttctgc tccgatggcg ctgagttggg ttccgacggg tccggtttgt
     5401 tgggaattga gatcgatgaa gctctcttac gagctgccat atcttattgc cccccgggat
     5461 cagatctgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa
     5521 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt
     5581 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg
     5641 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc
     5701 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc
     5761 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     5821 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     5881 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     5941 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
     6001 gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc
     6061 ggcagcgtga agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta
     6121 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     6181 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     6241 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     6301 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     6361 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     6421 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     6481 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     6541 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     6601 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     6661 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     6721 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     6781 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     6841 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     6901 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     6961 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     7021 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     7081 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     7141 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     7201 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     7261 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     7321 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     7381 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     7441 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     7501 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     7561 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     7621 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     7681 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7741 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7801 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7861 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7921 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7981 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8041 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8101 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8161 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8221 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8281 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8341 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8401 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8461 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8521 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8581 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8641 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8701 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8761 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     8821 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8881 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8941 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     9001 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     9061 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     9121 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     9181 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     9241 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     9301 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     9361 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     9421 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     9481 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9541 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9601 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     9661 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9721 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     9781 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9841 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9901 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9961 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
    10021 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
    10081 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
    10141 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
    10201 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    10261 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    10321 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
    10381 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    10441 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    10501 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10561 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10621 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10681 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10741 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    10801 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10861 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10921 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10981 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    11041 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    11101 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    11161 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    11221 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    11281 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    11341 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    11401 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    11461 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    11521 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11581 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11641 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11701 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    11761 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    11821 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11881 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11941 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    12001 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    12061 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    12121 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    12181 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    12241 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    12301 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    12361 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    12421 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    12481 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    12541 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    12601 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
