LOCUS       40924_23629       12033 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB670, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Development of Gateway Binary Vectors, pGWBn70, for Ectopic 
            Expression of Dominant Transcription Activators in Higher Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2013) Agricultural Bioscience, Nagoya University, 
            Fro-Cho Chikusa-Ku, Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 12033)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12033)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2013) Agricultural Bioscience, Nagoya University, Fro-Cho 
            Chikusa-Ku, Nagoya, Aichi 4648601, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1089..1155
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1170..1294
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1331..1361
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1415..2071
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2416..2718
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2762..2886)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2900..3133
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3155..3388
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      3419..3671
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3705..3721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3729..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3753..3783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3798..3819)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3855..4107)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4270..4818)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4870..5049)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5555..5579)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6100..6888
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7137..7725
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7911..8051)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8395..8589)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8658..9728)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10160..10786)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gagttatcaa caagtttgta caaaaaagct gaacgagaaa
     1201 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1261 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1321 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1381 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1441 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1501 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1561 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1621 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1681 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1741 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1801 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1861 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1921 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1981 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2041 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2101 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2161 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2221 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2281 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2341 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2401 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2461 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     2521 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     2581 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     2641 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     2701 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     2761 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     2821 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     2881 agtggttgat aacagcatgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     2941 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     3001 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     3061 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     3121 cgagtacggt ggggaattcc cgaagcttaa agtcgccccc ccgaccgatg tcagcctggg
     3181 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     3241 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     3301 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     3361 tgcccttgga attgacgagt acggtgggta atcgcgagct tagagctcga atttccccga
     3421 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     3481 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     3541 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     3601 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     3661 gttactagat cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct
     3721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3781 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     3841 ttctattaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     3901 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     3961 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     4021 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     4081 actttattgc caaatgtttg aacgatcggg gaaattcgag ctggggacgt ccccagctcg
     4141 gtaccccctc ctgggcacgg tcgtacgcga tccgccgcgg ttccacctca ccattgcggg
     4201 tatgccagtt gagttcgatg agttcccgta ccagctccaa ctcgggcgat gccacgcgca
     4261 ctccgttcag atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc
     4321 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
     4381 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
     4441 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
     4501 gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc
     4561 cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc
     4621 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
     4681 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
     4741 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
     4801 gcgtcgttct gggctcatat cttattgccc cccgggatca gatctggatt gagagtgaat
     4861 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     4921 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     4981 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     5041 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     5101 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     5161 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     5221 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     5281 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     5341 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     5401 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     5461 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     5521 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     5581 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     5641 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     5701 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     5761 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     5821 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     5881 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     5941 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     6001 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     6061 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     6121 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     6181 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     6241 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     6301 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     6361 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     6421 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     6481 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     6541 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     6601 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     6661 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     6721 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     6781 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     6841 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     6901 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     6961 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     7021 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     7081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7261 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     7921 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     7981 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8041 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8161 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     8221 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     8281 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     8341 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     8401 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     8461 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     8521 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     8581 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     8641 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     8701 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     8761 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     8821 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     8881 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     8941 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     9001 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     9061 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     9121 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     9181 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     9241 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     9301 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     9361 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     9421 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     9481 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     9541 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     9601 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     9661 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     9721 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     9781 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     9841 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     9901 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     9961 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10021 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10081 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    10141 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    10201 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    10261 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    10321 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    10381 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    10441 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    10501 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    10561 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    10621 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    10681 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    10741 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    10801 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    10861 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    10921 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    10981 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11041 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    11101 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    11161 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    11221 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    11281 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    11341 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    11401 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    11461 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    11521 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    11581 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    11641 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    11701 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    11761 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    11821 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    11881 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    11941 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    12001 catgcacata caaatggacg aacggataaa cct
//
