LOCUS       40924_23354       12498 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB570, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Development of Gateway Binary Vectors, pGWBn70, for Ectopic 
            Expression of Dominant Transcription Activators in Higher Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12498)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2013) Agricultural Bioscience, Nagoya University, 
            Fro-Cho Chikusa-Ku, Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 12498)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12498)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2013) Agricultural Bioscience, Nagoya University, Fro-Cho 
            Chikusa-Ku, Nagoya, Aichi 4648601, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1089..1155
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1170..1294
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1331..1361
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1415..2071
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2416..2718
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2762..2886)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2900..3133
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3155..3388
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      3419..3671
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3705..3721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3729..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3753..3783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3798..3819)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3855..4107)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4261..5283)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5335..5514)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6020..6044)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6565..7353
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7602..8190
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8376..8516)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8860..9054)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9123..10193)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10625..11251)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gagttatcaa caagtttgta caaaaaagct gaacgagaaa
     1201 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1261 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1321 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1381 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1441 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1501 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1561 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1621 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1681 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1741 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1801 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1861 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1921 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1981 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2041 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2101 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2161 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2221 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2281 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2341 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2401 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2461 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     2521 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     2581 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     2641 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     2701 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     2761 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     2821 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     2881 agtggttgat aacagcatgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     2941 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     3001 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     3061 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     3121 cgagtacggt ggggaattcc cgaagcttaa agtcgccccc ccgaccgatg tcagcctggg
     3181 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     3241 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     3301 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     3361 tgcccttgga attgacgagt acggtgggta atcgcgagct tagagctcga atttccccga
     3421 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     3481 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     3541 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     3601 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     3661 gttactagat cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct
     3721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3781 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     3841 ttctattaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     3901 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     3961 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     4021 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     4081 actttattgc caaatgtttg aacgatcggg gaaattcggg ggatcgatcc cgcgaactgt
     4141 ggacgagaac tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg
     4201 cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta
     4261 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
     4321 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
     4381 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
     4441 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
     4501 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
     4561 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
     4621 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     4681 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     4741 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     4801 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     4861 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     4921 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
     4981 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaatgc
     5041 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     5101 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     5161 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     5221 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
     5281 catatcttat tgccccccgg gatcagatct ggattgagag tgaatatgag actctaattg
     5341 gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag
     5401 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
     5461 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
     5521 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     5581 attctccgct catgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
     5641 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg
     5701 ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc
     5761 tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
     5821 catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca
     5881 gcccttgcgc cctgagtgct tgcggcagcg tgaagctgat ccgtcgagat taatagaaat
     5941 aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     6001 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     6061 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     6121 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     6181 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     6241 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     6301 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     6361 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     6421 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     6481 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     6541 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa
     6601 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     6661 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     6721 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     6781 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     6841 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     6901 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     6961 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     7021 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     7081 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     7141 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     7201 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     7261 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     7321 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag
     7381 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     7441 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     7501 ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc
     7561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7621 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7681 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7741 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7801 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7861 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7921 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7981 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8041 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8101 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8161 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8221 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8281 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8341 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     8401 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     8461 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     8521 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8581 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8641 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     8701 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     8761 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     8821 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
     8881 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
     8941 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
     9001 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
     9061 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
     9121 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
     9181 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
     9241 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
     9301 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
     9361 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
     9421 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
     9481 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
     9541 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
     9601 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
     9661 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
     9721 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
     9781 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
     9841 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
     9901 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
     9961 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
    10021 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
    10081 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
    10141 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
    10201 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
    10261 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    10321 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    10381 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    10441 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    10501 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    10561 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    10621 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    10681 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    10741 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    10801 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    10861 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    10921 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    10981 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    11041 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    11101 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    11161 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    11221 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    11281 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    11341 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    11401 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    11461 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    11521 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    11581 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    11641 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    11701 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    11761 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    11821 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    11881 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    11941 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    12001 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    12061 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    12121 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    12181 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    12241 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    12301 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    12361 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    12421 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    12481 ggacgaacgg ataaacct
//
