LOCUS       62056_11965       17961 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17961)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..17961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5824..5948
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5985..6015
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6069..6725
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             7070..7372
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     rep_origin      complement(7338..7962)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      8301..8553
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8982..9234)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9705..10727)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10737..11081)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(11825..11849)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13448..14593)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14895..15686)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15878..16645
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(17346..17956)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaat
     5821 caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     5881 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     5941 tcactatggc ggcccctcga ggggccgcat taggcacccc aggctttaca ctttatgctt
     6001 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
     6061 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     6121 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     6181 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     6241 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     6301 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     6361 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     6421 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     6481 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     6541 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     6601 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     6661 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     6721 gggcgtaatc tagaggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
     6781 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     6841 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     6901 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     6961 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     7021 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
     7081 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     7141 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     7201 taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     7261 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
     7321 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
     7381 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
     7441 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
     7501 cattttacgt ttctcgttca gctttcttgt acaaagtggt tcgatctaga ggatccatgg
     7561 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     7621 acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     7681 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     7741 tgaccacctt cacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     7801 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     7861 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     7921 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     7981 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     8041 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     8101 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     8161 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     8221 tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtaaagcg
     8281 gcccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     8341 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     8401 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     8461 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     8521 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt agcttcatca acgcaagaca
     8581 tgcgcacgac cgtctgacag gagaggaatt tccgacgagc acagaaagga cttgctcttg
     8641 gacgtaggcc tatttctcag gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg
     8701 tatcagccgc cgccaactgg gagatgagga ggctttcttg gggggcagtc agcagttcat
     8761 ttcacaagac agaggaactt gtaaggagat gcactgattt atcttggcgc aaaccagcag
     8821 gacgaattag tgggaatagc ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa
     8881 cttccaattc gaaacagttt ggagaggttg tttttgggca taccttttgt tagtcagcct
     8941 ctcgattgct catcgtcatt acacagtacc gaagtttgat cgatctagta acatagatga
     9001 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     9061 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     9121 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     9181 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttc
     9241 gacgcactcc ttctttactc caccatctcg tccttattga aaacgtgggt agcaccaaaa
     9301 cgaatcaagt cgctggaact gaagttacca atcacgctgg atgatttgcc agttggatta
     9361 atcttgcctt tccccgcatg aataatattg atgaatgcat gcgtgagggg tatttcgatt
     9421 ttggcaatag ctgcaattgc cgcgacatcc tccaacgagc ataattcttc agaaaaatag
     9481 cgatgttcca tgttgtcagg gcatgcatga tgcacgttat gaggtgacgg tgctaggcag
     9541 tattccctca aagtttcata gtcagtatca tattcatcat tgcattcctg caagagagaa
     9601 ttgagacgca atccacacgc tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg
     9661 acgttgcaaa cgtaagtgtt ggatcccggt cggcatctac tctattcctt tgccctcgga
     9721 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     9781 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     9841 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     9901 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca
     9961 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
    10021 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
    10081 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
    10141 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    10201 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    10261 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    10321 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc
    10381 gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    10441 tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact
    10501 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    10561 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    10621 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    10681 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc
    10741 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
    10801 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg
    10861 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
    10921 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
    10981 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
    11041 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
    11101 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacgg
    11161 atctctagga cgcgtcctag aagctaattc actggccgtc gttttacaac gtcgtgactg
    11221 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    11281 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    11341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    11401 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    11461 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    11521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    11581 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
    11641 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
    11701 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
    11761 aaaaaccacc ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
    11821 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
    11881 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
    11941 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
    12001 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
    12061 atgacagagc gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc
    12121 agccttctta ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg
    12181 acataatagg aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag
    12241 aagtgaacgt tgacgatatc aactccccta tccattgctc accgaatggt acaggtcggg
    12301 gacccgaagt tccgactgtc ggcctgatgc atccccggct gatcgacccc agatctgggg
    12361 ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa
    12421 aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt
    12481 cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct
    12541 ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg
    12601 gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca
    12661 ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata
    12721 gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg
    12781 accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc
    12841 gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc
    12901 atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc
    12961 aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg
    13021 ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc
    13081 ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg
    13141 aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct
    13201 ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt
    13261 cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
    13321 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
    13381 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
    13441 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
    13501 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
    13561 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
    13621 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
    13681 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
    13741 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
    13801 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
    13861 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
    13921 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
    13981 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
    14041 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
    14101 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
    14161 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
    14221 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
    14281 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
    14341 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
    14401 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
    14461 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
    14521 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
    14581 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
    14641 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
    14701 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
    14761 cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc
    14821 caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc
    14881 acatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
    14941 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
    15001 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
    15061 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
    15121 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
    15181 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
    15241 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
    15301 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
    15361 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
    15421 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
    15481 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
    15541 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
    15601 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
    15661 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
    15721 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    15781 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    15841 tagtatcgac ggagccgatt ttgaaaccac aattatgggt gatgctgcca acttactgat
    15901 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtgtctatc agctgtccct
    15961 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
    16021 atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac
    16081 ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag
    16141 cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca
    16201 gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga
    16261 acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag
    16321 tctccggaag acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg
    16381 tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct
    16441 gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat
    16501 tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc
    16561 gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca
    16621 ctatcaataa gttggagtca ttacccaatt atgatagaat ttacaagcta taaggttatt
    16681 gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat
    16741 attgataagc gcgctgccta tgccttgccc cctgaaatcc ttacatacgg cgatatcttc
    16801 tatataaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct
    16861 catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt
    16921 ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg
    16981 gttcgctggg tttatcgcac ccccgaacac gagcacggca cccgcgacca ctatgccaag
    17041 aatgcccaag gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga
    17101 agtgaagggc aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa
    17161 tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc
    17221 ccatcccgtt actgccccga tcccggcaat ggcaaggact gccagcgctg ccatttttgg
    17281 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc
    17341 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca
    17401 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga
    17461 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg
    17521 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa
    17581 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca
    17641 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt
    17701 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct
    17761 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc
    17821 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg
    17881 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag
    17941 cggcgagggc aaccagcccg g
//
