LOCUS       40924_23079       12177 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB470, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Development of Gateway Binary Vectors, pGWBn70, for Ectopic 
            Expression of Dominant Transcription Activators in Higher Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12177)
  AUTHORS   Tsukagoshi H, Kawase AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2013) Agricultural Bioscience, Nagoya University, 
            Fro-Cho Chikusa-Ku, Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 12177)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12177)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2013) Agricultural Bioscience, Nagoya University, Fro-Cho 
            Chikusa-Ku, Nagoya, Aichi 4648601, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1089..1155
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1170..1294
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1331..1361
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1415..2071
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2416..2718
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2762..2886)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2900..3133
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3155..3388
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      3419..3671
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3705..3721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3729..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3753..3783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3798..3819)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3853..4105)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4498..5286)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5310..5493)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5699..5723)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6244..7032
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7281..7869
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8055..8195)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8539..8733)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8802..9872)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10304..10930)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gagttatcaa caagtttgta caaaaaagct gaacgagaaa
     1201 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1261 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1321 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1381 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1441 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1501 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1561 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1621 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1681 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1741 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1801 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1861 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1921 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1981 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2041 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2101 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2161 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2221 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2281 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2341 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2401 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2461 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     2521 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     2581 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     2641 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     2701 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     2761 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     2821 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     2881 agtggttgat aacagcatgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     2941 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     3001 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     3061 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     3121 cgagtacggt ggggaattcc cgaagcttaa agtcgccccc ccgaccgatg tcagcctggg
     3181 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     3241 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     3301 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     3361 tgcccttgga attgacgagt acggtgggta atcgcgagct tagagctcga atttccccga
     3421 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     3481 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     3541 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     3601 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     3661 gttactagat cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct
     3721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3781 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     3841 ttctgaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     3901 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     3961 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     4021 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     4081 tttattgcca aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg aactccacga
     4141 aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc
     4201 cgacgccgtt gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt
     4261 gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagaga
     4321 tcccgtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     4381 cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     4441 gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa
     4501 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     4561 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     4621 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     4681 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     4741 atcatcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     4801 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     4861 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     4921 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     4981 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     5041 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     5101 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     5161 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     5221 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     5281 ttcaatcatg cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga
     5341 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
     5401 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     5461 gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc
     5521 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     5581 tgactccctt aattctccgc tcatgatgaa ttcagaaata aattcagcct aattcggcgt
     5641 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     5701 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     5761 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     5821 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     5881 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     5941 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     6001 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     6061 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     6121 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     6181 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     6241 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     6301 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     6361 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     6421 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     6481 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     6541 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     6601 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     6661 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     6721 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     6781 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     6841 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     6901 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     6961 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     7021 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     7081 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     7141 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     7201 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7261 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7321 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7381 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7441 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7501 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7561 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7621 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7681 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7741 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7801 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7861 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7921 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7981 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8041 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     8101 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     8161 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     8221 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8281 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     8341 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     8401 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     8461 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     8521 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     8581 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
     8641 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
     8701 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
     8761 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
     8821 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
     8881 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
     8941 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
     9001 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
     9061 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
     9121 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
     9181 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
     9241 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
     9301 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
     9361 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
     9421 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
     9481 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
     9541 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
     9601 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
     9661 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
     9721 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
     9781 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
     9841 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
     9901 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
     9961 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    10021 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    10081 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    10141 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    10201 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    10261 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    10321 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    10381 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    10441 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    10501 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    10561 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    10621 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    10681 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    10741 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    10801 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    10861 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    10921 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    10981 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    11041 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    11101 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    11161 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    11221 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    11281 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    11341 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    11401 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    11461 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    11521 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    11581 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    11641 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    11701 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    11761 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    11821 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    11881 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    11941 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    12001 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    12061 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    12121 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
