LOCUS       62056_11960       17102 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17102)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..17102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4977..5101
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5138..5168
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5222..5878
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6223..6525
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     rep_origin      complement(6479..7103)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(8123..8375)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8846..9868)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9878..10222)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(10966..10990)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12589..13734)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14036..14827)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15019..15786
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(16487..17097)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggct ctaatcacaa
     4981 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     5041 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     5101 ggcggcccct cgaggggccg cattaggcac cccaggcttt acactttatg cttccggctc
     5161 gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa
     5221 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     5281 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     5341 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     5401 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     5461 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     5521 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     5581 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     5641 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     5701 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     5761 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
     5821 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     5881 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     5941 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct
     6001 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     6061 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     6121 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     6181 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     6241 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     6301 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     6361 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     6421 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     6481 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     6541 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     6601 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     6661 cgtttctcgt tcagctttct tgtacaaagt ggtgatcatg gtgagcaagg gcgaggagct
     6721 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     6781 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6841 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     6901 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6961 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     7021 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     7081 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     7141 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     7201 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     7261 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     7321 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     7381 cgggatcact cacggcatgg acgagctgta caagtaaagc ggcccgagct cgaatttccc
     7441 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     7501 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     7561 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     7621 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     7681 tatgttacta gatcgggaat tagcttcatc aacgcaagac atgcgcacga ccgtctgaca
     7741 ggagaggaat ttccgacgag cacagaaagg acttgctctt ggacgtaggc ctatttctca
     7801 ggcacatgta tcaagtgttc ggacgtgggt tttcgatggt gtatcagccg ccgccaactg
     7861 ggagatgagg aggctttctt ggggggcagt cagcagttca tttcacaaga cagaggaact
     7921 tgtaaggaga tgcactgatt tatcttggcg caaaccagca ggacgaatta gtgggaatag
     7981 cccgcgaata tctaagttat gcctgtcggc atgagcagaa acttccaatt cgaaacagtt
     8041 tggagaggtt gtttttgggc ataccttttg ttagtcagcc tctcgattgc tcatcgtcat
     8101 tacacagtac cgaagtttga tcgatctagt aacatagatg acaccgcgcg cgataattta
     8161 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     8221 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     8281 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     8341 cttaagaaac tttattgcca aatgtttgaa cgatctgctt cgacgcactc cttctttact
     8401 ccaccatctc gtccttattg aaaacgtggg tagcaccaaa acgaatcaag tcgctggaac
     8461 tgaagttacc aatcacgctg gatgatttgc cagttggatt aatcttgcct ttccccgcat
     8521 gaataatatt gatgaatgca tgcgtgaggg gtatttcgat tttggcaata gctgcaattg
     8581 ccgcgacatc ctccaacgag cataattctt cagaaaaata gcgatgttcc atgttgtcag
     8641 ggcatgcatg atgcacgtta tgaggtgacg gtgctaggca gtattccctc aaagtttcat
     8701 agtcagtatc atattcatca ttgcattcct gcaagagaga attgagacgc aatccacacg
     8761 ctgcggcaac cttccggcgt tcgtggtcta tttgctcttg gacgttgcaa acgtaagtgt
     8821 tggatcccgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt
     8881 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     8941 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     9001 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     9061 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     9121 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     9181 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     9241 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     9301 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     9361 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     9421 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     9481 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     9541 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     9601 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     9661 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     9721 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     9781 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     9841 acgtcgcggt gagttcaggc tttttcatat cggggtcgtc ctctccaaat gaaatgaact
     9901 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     9961 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    10021 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    10081 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    10141 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    10201 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    10261 cttggagtag acgagagtgt cgtgctccac catgttgacg gatctctagg acgcgtccta
    10321 gaagctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    10381 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    10441 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt
    10501 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10561 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
    10621 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    10681 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    10741 gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat
    10801 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
    10861 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat
    10921 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
    10981 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
    11041 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
    11101 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
    11161 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
    11221 tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct
    11281 cgcttaaccg tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg
    11341 gataaagccg ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatat
    11401 caactcccct atccattgct caccgaatgg tacaggtcgg ggacccgaag ttccgactgt
    11461 cggcctgatg catccccggc tgatcgaccc cagatctggg gctgagaaag cccagtaagg
    11521 aaacaactgt aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg
    11581 ctccgatcag gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg
    11641 gcggatcaaa cactaaagct actggaacga gcagaagtcc tccggccgcc agttgccagg
    11701 cggtaaaggt gagcagaggc acgggaggtt gccacttgcg ggtcagcacg gttccgaacg
    11761 ccatggaaac cgcccccgcc aggcccgctg cgacgccgac aggatctagc gctgcgtttg
    11821 gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat agcccccagg accgccatca
    11881 atcgtatcgg gctacctagc agagcggcag agatgaacac gaccatcagc ggctgcacag
    11941 cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac cgaaataaac aacaagctcc
    12001 agaatagcga aatattaagt gcgccgagga tgaagatgcg catccaccag attcccgttg
    12061 gaatctgtcg gacgatcatc acgagcaata aacccgccgg caacgcccgc agcagcatac
    12121 cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt
    12181 gagcgtcctt ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga
    12241 tgtaggcgcc gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt
    12301 tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc
    12361 ggatctcgcg gaaatcctgc acgtcggccg ctccaagccg tcgaatctga gccttaatca
    12421 caattgtcaa ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga
    12481 gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa
    12541 agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
    12601 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
    12661 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
    12721 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
    12781 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
    12841 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
    12901 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
    12961 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
    13021 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
    13081 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
    13141 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
    13201 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
    13261 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
    13321 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
    13381 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
    13441 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag
    13501 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
    13561 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
    13621 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
    13681 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
    13741 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc
    13801 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac
    13861 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga
    13921 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa
    13981 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca
    14041 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
    14101 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
    14161 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
    14221 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
    14281 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
    14341 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
    14401 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
    14461 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
    14521 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
    14581 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
    14641 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
    14701 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
    14761 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
    14821 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
    14881 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
    14941 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
    15001 tttgaaacca caattatggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
    15061 ttgaggtgct ccagtggctt ctgtgtctat cagctgtccc tcctgttcag ctactgacgg
    15121 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg
    15181 taaaacatgg caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat
    15241 cattgatatg gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg
    15301 cctcaacacg attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata
    15361 cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg
    15421 gctaaatcgc gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt
    15481 gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca
    15541 ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag
    15601 ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat
    15661 ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag
    15721 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
    15781 attacccaat tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt
    15841 agtccatgca agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct
    15901 atgccttgcc ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat
    15961 cttatcagta ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc
    16021 gtcttggtag ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc
    16081 gttttcatac ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttatcgca
    16141 cccccgaaca cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt
    16201 gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca
    16261 cccaggccgc cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc
    16321 ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg
    16381 atcccggcaa tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc
    16441 gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga
    16501 agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa
    16561 aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa
    16621 aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt
    16681 caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc
    16741 tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct
    16801 tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc
    16861 tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc
    16921 gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt
    16981 ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt
    17041 ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc
    17101 gg
//
