LOCUS       62056_11955       17643 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..17643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5842..5871
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     protein_bind    6241..6365
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6402..6432
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6486..7142
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      complement(7020..7646)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    complement(7833..7957)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      7983..8235
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8664..8916)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9387..10409)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10419..10763)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(11507..11531)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13130..14275)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14577..15368)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15560..16327
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(17028..17638)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 atgagcgggt taattaacgg tgaacaaaag ctaatctccg aggaagactt gaacggtgaa
     5881 caaaaattaa tctcagaaga agacttgaac ggactcgacg gtgaacaaaa gttgatttct
     5941 gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa cggtgaacaa
     6001 aaattaatct cagaagaaga cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa
     6061 gaagatttga acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa
     6121 ttaatctcag aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa
     6181 gatttgaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtag cgctgttatc
     6241 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     6301 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     6361 ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt tatgcttccg
     6421 gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga gctaaggaag
     6481 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     6541 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     6601 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     6661 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     6721 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     6781 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     6841 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     6901 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     6961 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     7021 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct
     7081 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     7141 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     7201 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg
     7261 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     7321 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     7381 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     7441 attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt
     7501 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     7561 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     7621 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     7681 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     7741 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     7801 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     7861 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     7921 tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aacctagagc tcgaatttcc
     7981 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     8041 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     8101 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     8161 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     8221 ctatgttact agatcgggaa ttagcttcat caacgcaaga catgcgcacg accgtctgac
     8281 aggagaggaa tttccgacga gcacagaaag gacttgctct tggacgtagg cctatttctc
     8341 aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg tgtatcagcc gccgccaact
     8401 gggagatgag gaggctttct tggggggcag tcagcagttc atttcacaag acagaggaac
     8461 ttgtaaggag atgcactgat ttatcttggc gcaaaccagc aggacgaatt agtgggaata
     8521 gcccgcgaat atctaagtta tgcctgtcgg catgagcaga aacttccaat tcgaaacagt
     8581 ttggagaggt tgtttttggg catacctttt gttagtcagc ctctcgattg ctcatcgtca
     8641 ttacacagta ccgaagtttg atcgatctag taacatagat gacaccgcgc gcgataattt
     8701 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac
     8761 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg
     8821 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa
     8881 tcttaagaaa ctttattgcc aaatgtttga acgatctgct tcgacgcact ccttctttac
     8941 tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa aacgaatcaa gtcgctggaa
     9001 ctgaagttac caatcacgct ggatgatttg ccagttggat taatcttgcc tttccccgca
     9061 tgaataatat tgatgaatgc atgcgtgagg ggtatttcga ttttggcaat agctgcaatt
     9121 gccgcgacat cctccaacga gcataattct tcagaaaaat agcgatgttc catgttgtca
     9181 gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc agtattccct caaagtttca
     9241 tagtcagtat catattcatc attgcattcc tgcaagagag aattgagacg caatccacac
     9301 gctgcggcaa ccttccggcg ttcgtggtct atttgctctt ggacgttgca aacgtaagtg
     9361 ttggatcccg gtcggcatct actctattcc tttgccctcg gacgagtgct ggggcgtcgg
     9421 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     9481 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     9541 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     9601 ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
     9661 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     9721 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     9781 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     9841 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     9901 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     9961 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
    10021 gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
    10081 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
    10141 caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg
    10201 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
    10261 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
    10321 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
    10381 gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt cctctccaaa tgaaatgaac
    10441 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
    10501 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
    10561 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
    10621 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
    10681 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
    10741 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
    10801 tcttggagta gacgagagtg tcgtgctcca ccatgttgac ggatctctag gacgcgtcct
    10861 agaagctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    10921 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    10981 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct cctttcgctt
    11041 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    11101 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
    11161 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    11221 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    11281 cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca tcaaacagga
    11341 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
    11401 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccccagtaca
    11461 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
    11521 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
    11581 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
    11641 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
    11701 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
    11761 ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct tattcatttc
    11821 tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata ggaaatcgct
    11881 ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac gttgacgata
    11941 tcaactcccc tatccattgc tcaccgaatg gtacaggtcg gggacccgaa gttccgactg
    12001 tcggcctgat gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag
    12061 gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc
    12121 gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt
    12181 ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag
    12241 gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac
    12301 gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt
    12361 ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc
    12421 aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca
    12481 gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc
    12541 cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt
    12601 ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcagcata
    12661 ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag atgcgccttg
    12721 tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg
    12781 atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc catgggcttt
    12841 ttctcctcgt gctcgtaaac ggacccgaac atctctggag ctttcttcag ggccgacaat
    12901 cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg agccttaatc
    12961 acaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg
    13021 agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta
    13081 aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat
    13141 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
    13201 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
    13261 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
    13321 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
    13381 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
    13441 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
    13501 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
    13561 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
    13621 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
    13681 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
    13741 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
    13801 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
    13861 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
    13921 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
    13981 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
    14041 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
    14101 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
    14161 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
    14221 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
    14281 ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc
    14341 cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga
    14401 ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg
    14461 accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga
    14521 agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gtactaaaac
    14581 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    14641 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    14701 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    14761 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    14821 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    14881 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    14941 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    15001 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    15061 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    15121 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    15181 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    15241 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    15301 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    15361 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    15421 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    15481 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    15541 ttttgaaacc acaattatgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
    15601 tttgaggtgc tccagtggct tctgtgtcta tcagctgtcc ctcctgttca gctactgacg
    15661 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc
    15721 gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa
    15781 tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt atgggcgttg
    15841 gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat
    15901 acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg
    15961 ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga agacggttgt
    16021 tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc
    16081 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa
    16141 gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa
    16201 tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga
    16261 gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat aagttggagt
    16321 cattacccaa ttatgataga atttacaagc tataaggtta ttgtcctggg tttcaagcat
    16381 tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa gcgcgctgcc
    16441 tatgccttgc cccctgaaat ccttacatac ggcgatatct tctatataaa agatatatta
    16501 tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct tcatcctctt
    16561 cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag gtccaattct
    16621 cgttttcata cctcggtata atcttaccta tcacctcaaa tggttcgctg ggtttatcgc
    16681 acccccgaac acgagcacgg cacccgcgac cactatgcca agaatgccca aggtaaaaat
    16741 tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc
    16801 acccaggccg ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg
    16861 cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc
    16921 gatcccggca atggcaagga ctgccagcgc tgccattttt ggggtgaggc cgttcgcggc
    16981 cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag
    17041 aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa
    17101 aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga
    17161 aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg
    17221 tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc
    17281 ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc
    17341 ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg
    17401 ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc
    17461 cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt
    17521 tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg
    17581 tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc
    17641 cgg
//
