LOCUS       40924_22829       17407 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 17407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..17407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     terminator      4022..4274
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5828..5952
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5989..6019
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6073..6729
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     rep_origin      complement(6784..7408)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    complement(7420..7544)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7570..7599
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             7606..7635
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             7651..7680
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             7687..7716
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="Myc"
                     /translation="EQKLISEEDL"
     terminator      7747..7999
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8428..8680)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9151..10173)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10183..10527)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10637..10653)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(11271..11295)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12894..14039)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(14341..15132)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     mobile_element  15324..16091
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(16792..17402)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gttatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     5881 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     5941 ccagtcacta tggcggcccc tcgaggggcc gcattaggca ccccaggctt tacactttat
     6001 gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt ttcaggagct
     6061 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     6121 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     6181 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     6241 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     6301 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     6361 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     6421 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     6481 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     6541 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     6601 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     6661 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     6721 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     6781 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     6841 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     6901 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     6961 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     7021 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
     7081 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     7141 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     7201 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     7261 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     7321 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     7381 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     7441 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     7501 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgataa cagcgggtta
     7561 attaacggtg aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc
     7621 tcagaagaag acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg
     7681 aacggtgaac aaaagctaat ctccgaggaa gacttgaacg gtagcgctta gagctcgaat
     7741 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     7801 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     7861 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     7921 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     7981 tcatctatgt tactagatcg ggaattagct tcatcaacgc aagacatgcg cacgaccgtc
     8041 tgacaggaga ggaatttccg acgagcacag aaaggacttg ctcttggacg taggcctatt
     8101 tctcaggcac atgtatcaag tgttcggacg tgggttttcg atggtgtatc agccgccgcc
     8161 aactgggaga tgaggaggct ttcttggggg gcagtcagca gttcatttca caagacagag
     8221 gaacttgtaa ggagatgcac tgatttatct tggcgcaaac cagcaggacg aattagtggg
     8281 aatagcccgc gaatatctaa gttatgcctg tcggcatgag cagaaacttc caattcgaaa
     8341 cagtttggag aggttgtttt tgggcatacc ttttgttagt cagcctctcg attgctcatc
     8401 gtcattacac agtaccgaag tttgatcgat ctagtaacat agatgacacc gcgcgcgata
     8461 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
     8521 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
     8581 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
     8641 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttcgacg cactccttct
     8701 ttactccacc atctcgtcct tattgaaaac gtgggtagca ccaaaacgaa tcaagtcgct
     8761 ggaactgaag ttaccaatca cgctggatga tttgccagtt ggattaatct tgcctttccc
     8821 cgcatgaata atattgatga atgcatgcgt gaggggtatt tcgattttgg caatagctgc
     8881 aattgccgcg acatcctcca acgagcataa ttcttcagaa aaatagcgat gttccatgtt
     8941 gtcagggcat gcatgatgca cgttatgagg tgacggtgct aggcagtatt ccctcaaagt
     9001 ttcatagtca gtatcatatt catcattgca ttcctgcaag agagaattga gacgcaatcc
     9061 acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc tcttggacgt tgcaaacgta
     9121 agtgttggat cccggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg
     9181 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
     9241 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
     9301 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
     9361 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
     9421 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
     9481 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
     9541 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
     9601 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     9661 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     9721 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     9781 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     9841 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
     9901 gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag
     9961 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    10021 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    10081 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    10141 gacagacgtc gcggtgagtt caggcttttt catatcgggg tcgtcctctc caaatgaaat
    10201 gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt
    10261 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
    10321 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
    10381 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
    10441 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
    10501 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
    10561 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tgacggatct ctaggacgcg
    10621 tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
    10681 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10741 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
    10801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10861 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10921 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10981 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11041 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
    11101 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
    11161 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
    11221 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    11281 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    11341 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    11401 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    11461 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    11521 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
    11581 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
    11641 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
    11701 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
    11761 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
    11821 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
    11881 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
    11941 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
    12001 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
    12061 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
    12121 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
    12181 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
    12241 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
    12301 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
    12361 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
    12421 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
    12481 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
    12541 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
    12601 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
    12661 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
    12721 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
    12781 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
    12841 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
    12901 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
    12961 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
    13021 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
    13081 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
    13141 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
    13201 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
    13261 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
    13321 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
    13381 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
    13441 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
    13501 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
    13561 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
    13621 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
    13681 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
    13741 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
    13801 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
    13861 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
    13921 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    13981 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    14041 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    14101 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    14161 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    14221 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    14281 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    14341 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    14401 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    14461 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    14521 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    14581 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    14641 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    14701 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    14761 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    14821 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    14881 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    14941 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    15001 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    15061 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    15121 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    15181 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    15241 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    15301 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    15361 tgtttttgag gtgctccagt ggcttctgtg tctatcagct gtccctcctg ttcagctact
    15421 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    15481 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    15541 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    15601 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    15661 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    15721 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    15781 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    15841 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    15901 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    15961 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    16021 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    16081 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    16141 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    16201 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    16261 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    16321 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    16381 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    16441 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    16501 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    16561 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    16621 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    16681 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    16741 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    16801 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    16861 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    16921 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    16981 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    17041 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    17101 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    17161 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    17221 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    17281 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    17341 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    17401 agcccgg
//
