LOCUS       62056_11950       17262 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17262)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..17262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5827..5850
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     protein_bind    5860..5984
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6021..6051
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6105..6761
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      complement(6639..7265)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    complement(7452..7576)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      7602..7854
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8283..8535)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9006..10028)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10038..10382)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(11126..11150)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12749..13894)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14196..14987)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15179..15946
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(16647..17257)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 atgagcgact acaaggatga cgatgacaag gctgttatca caagtttgta caaaaaagct
     5881 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     5941 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg
     6001 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     6061 tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     6121 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     6181 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     6241 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     6301 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     6361 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     6421 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     6481 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     6541 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     6601 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     6661 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     6721 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct
     6781 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     6841 atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
     6901 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     6961 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     7021 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     7081 gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     7141 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     7201 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     7261 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     7321 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     7381 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     7441 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     7501 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     7561 tcttgtacaa agtggtgata acctagagct cgaatttccc cgatcgttca aacatttggc
     7621 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     7681 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     7741 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     7801 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     7861 tagcttcatc aacgcaagac atgcgcacga ccgtctgaca ggagaggaat ttccgacgag
     7921 cacagaaagg acttgctctt ggacgtaggc ctatttctca ggcacatgta tcaagtgttc
     7981 ggacgtgggt tttcgatggt gtatcagccg ccgccaactg ggagatgagg aggctttctt
     8041 ggggggcagt cagcagttca tttcacaaga cagaggaact tgtaaggaga tgcactgatt
     8101 tatcttggcg caaaccagca ggacgaatta gtgggaatag cccgcgaata tctaagttat
     8161 gcctgtcggc atgagcagaa acttccaatt cgaaacagtt tggagaggtt gtttttgggc
     8221 ataccttttg ttagtcagcc tctcgattgc tcatcgtcat tacacagtac cgaagtttga
     8281 tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     8341 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     8401 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     8461 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     8521 aatgtttgaa cgatctgctt cgacgcactc cttctttact ccaccatctc gtccttattg
     8581 aaaacgtggg tagcaccaaa acgaatcaag tcgctggaac tgaagttacc aatcacgctg
     8641 gatgatttgc cagttggatt aatcttgcct ttccccgcat gaataatatt gatgaatgca
     8701 tgcgtgaggg gtatttcgat tttggcaata gctgcaattg ccgcgacatc ctccaacgag
     8761 cataattctt cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg atgcacgtta
     8821 tgaggtgacg gtgctaggca gtattccctc aaagtttcat agtcagtatc atattcatca
     8881 ttgcattcct gcaagagaga attgagacgc aatccacacg ctgcggcaac cttccggcgt
     8941 tcgtggtcta tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg tcggcatcta
     9001 ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     9061 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     9121 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     9181 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     9241 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     9301 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     9361 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     9421 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     9481 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     9541 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     9601 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     9661 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     9721 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     9781 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     9841 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     9901 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     9961 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
    10021 tttttcatat cggggtcgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
    10081 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    10141 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    10201 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    10261 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    10321 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    10381 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    10441 cgtgctccac catgttgacg gatctctagg acgcgtccta gaagctaatt cactggccgt
    10501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10621 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    10681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    10741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
    10801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    10861 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
    10921 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
    10981 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
    11041 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
    11101 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
    11161 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
    11221 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
    11281 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
    11341 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
    11401 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
    11461 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
    11521 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
    11581 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
    11641 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
    11701 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
    11761 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
    11821 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
    11881 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
    11941 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
    12001 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
    12061 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
    12121 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
    12181 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
    12241 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
    12301 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
    12361 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
    12421 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
    12481 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
    12541 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
    12601 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
    12661 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
    12721 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    12781 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    12841 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    12901 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    12961 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    13021 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    13081 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    13141 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    13201 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    13261 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    13321 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    13381 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    13441 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    13501 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    13561 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    13621 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    13681 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    13741 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    13801 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    13861 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    13921 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    13981 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    14041 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    14101 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    14161 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    14221 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    14281 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    14341 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    14401 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    14461 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    14521 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    14581 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    14641 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    14701 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    14761 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    14821 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    14881 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    14941 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    15001 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    15061 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    15121 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    15181 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    15241 ctgtgtctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    15301 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    15361 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    15421 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    15481 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    15541 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    15601 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    15661 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    15721 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    15781 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    15841 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    15901 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    15961 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    16021 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    16081 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    16141 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    16201 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    16261 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    16321 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    16381 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    16441 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    16501 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    16561 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    16621 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    16681 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    16741 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    16801 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    16861 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    16921 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    16981 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    17041 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    17101 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    17161 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    17221 catagacggc cgccagccca gcggcgaggg caaccagccc gg
//
