LOCUS       V005863                 6709 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005863
VERSION     V005863
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Girbal L, Mortier-Barriere I, Raynaud F, Rouanet C, Croux C,
            Soucaille P.
  TITLE     Development of a sensitive gene expression reporter system and an
            inducible promoter-repressor system for Clostridium acetobutylicum
  JOURNAL   Appl. Environ. Microbiol. 69 (8), 4985-4988 (2003)
   PUBMED   12902297
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Girbal L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2003) Laboratoire de Biotechnologie-Bioprocedes,
            UMR CNRS 5504, UR INRA 792, INSA, 135, avenue de Rangueil, Toulouse
            31077, France
REFERENCE   3  (bases 1 to 6709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2003"; volume: "69"; issue: "8"; pages:
            "4985-4988"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2003) Laboratoire de Biotechnologie-Bioprocedes, UMR CNRS
            5504, UR INRA 792, INSA, 135, avenue de Rangueil, Toulouse 31077,
            France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..1828
                     /codon_start=1
                     /gene="gusA"
                     /product="beta-glucuronidase"
                     /label="gusA"
                     /note="from Escherichia coli; UidA"
                     /protein_id="AAQ21188.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            20..1828
                     /gene="gusA"
                     /label="gusA"
     primer_bind     complement(2008..2024)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2032..2048)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2056..2086)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2101..2122)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2410..2998)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3172..4029)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4030..4134)
                     /label="AmpR promoter"
     primer_bind     4610..4626
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4694..5134)
                     /codon_start=1
                     /gene="repL"
                     /product="RepL"
                     /label="repL"
                     /note="Bacillus subtilis plasmid pIM13 replication protein"
                     /protein_id="AAQ21190.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            complement(4694..5134)
                     /gene="repL"
                     /label="repL"
     CDS             complement(5585..6316)
                     /gene="ermC'"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Bacillus
                     subtilis. Accession#: P13956"
ORIGIN
        1 ggtttaggag gttagttata tgttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa
       61 actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg
      121 gtgggaaagc gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca
      181 gttcgccgat gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt
      241 tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta
      301 cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt
      361 tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt
      421 gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg
      481 caagaaaaag cagtcttact tccatgattt ctttaactat gccgggatcc atcgcagcgt
      541 aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc
      601 gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt
      661 tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt
      721 gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt
      781 cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt
      841 ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg
      901 tcgtcatgaa gatgcggact tacgtggcaa aggattcgat aacgtgctga tggtgcacga
      961 ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc
     1021 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc
     1081 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact
     1141 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga
     1201 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc
     1261 ggatacccgt ccgcaagtgc acgggaatat ttcgccactg gcggaagcaa cgcgtaaact
     1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     1801 aaaaccgcag cagggaggca aacaatgaat cggcgccact taatgatttg ccagtaaaag
     1861 agattgtttc tagctctcac attcttgcag atataatatt gcctagagct gaagttatat
     1921 atgattatct taagtaataa aaataagagt taccttaaat ggtaactctt atttttttaa
     1981 tgtcgactca tagaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2041 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2581 ttcgggaagc gtggcgcttt ctcaaagctc acgctgtagg tatctcagtt cggtgtaggt
     2641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4141 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     4201 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     4261 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4321 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     4381 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     4441 cgtaaggaga aaataccgca tcaggcgcgc cattcgccat tcaggctgcg caactgttgg
     4501 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     4561 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     4621 gccagtgcca agcttgagct taatcgcatt tcatagattg acctcccaat aactacgtgg
     4681 tgttattggg aggtcaatct atttcatttg cctcttgctc aaagttccca aattcgagta
     4741 agaggtattt ttgtttttgg tcgtcgcctc tcattagtag ttcagggttt aacattaata
     4801 ctccagtttt tctttttata atatttcctt cttctaagat tttaagtgtt gttattactg
     4861 tttgtagact tgttcctgta gcttttgcta tttctcttgt tgtagctatc attgtattgt
     4921 tacttaagtg gacattatct aggatatagt taacgatttt aagttttttt ccgccaatca
     4981 tatctaacat acttattaat tgcactatat atgcctttac gaagttacca gacgtttgtt
     5041 tacggtataa cttgtctacc tctatgactt ctccactttc ttcgtctatg agcctctgag
     5101 agcctttata gactgttcca tatctttctt tcatcttttt ctcactcctt attttaaact
     5161 attctaacta tatcataact gttctaaaaa aaaaagaaca tttgttaaaa gaaattagaa
     5221 caaaatgagt gaaaaattag aacaaacaaa ttccttataa accttatcat ctcaacctat
     5281 attaagattt tacctagttg aatcttcttt tctatataaa gcgtcggagc atatcagggg
     5341 gttatctaac gtaaatgcta cccttcggct cgctttcgct cggcattgac gtcagatact
     5401 gcaccccctg aacccccatg ctccaacagc aaaaaggaaa ctttttgctg cttttccgac
     5461 gcttattcgc ttcgctcata tttatataga aaagaagtga atgcgcaaaa gacataatcg
     5521 attcacaaaa aataggtaca cgaaaaacaa gttaagggat gcagtttatg catcccttaa
     5581 cttacttatt aaataattta tagctattga aaagagataa gaattgttca aagctaatat
     5641 tgtttaaatc gtcaattcct gcatgtttta aggaattgtt aaattgattt tttgtaaata
     5701 ttttcttgta ttctttgtta acccatttca taacgaaata attatacttc tgtttatctt
     5761 tgtgtgatat tcttgatttt tttctattta atctgataag tgagctattc actttaggtt
     5821 taggatgaaa atattctctt ggaaccatac ttaatataga aatatcaact tctgccatta
     5881 aaaataatgc caatgagcgt tttgtattta ataatctttt agcaaacccg tattccacga
     5941 ttaaataaat ctcatcagct atactatcaa aaacaatttt gcgtattata tccgtactta
     6001 tgttataagg tatattacca aatattttat aggattggtt tttaggaaat ttaaactgca
     6061 atatatcctt gtttaaaact tggaaattat cgtgatcaac aagtttattt tctgtagttt
     6121 tgcataattt atggtctatt tcaatggcag ttacgaaatt acacctctgt actaattcaa
     6181 gggtaaaatg cccttttcct gagccgattt caaagatatt atcatgttca tttaatctta
     6241 tatttgtcat tattttatct atattatgtt ttgaagtaat aaagttttga ctgtgtttta
     6301 tatttttctc gttcattata accctcttta ttttttcctc cttataaaat tagtataatt
     6361 atagcacgag ctctgataaa tatgaacatg atgagtgatc gttaaattta tactgcaatc
     6421 tgatgcgatt attgaataaa agatatgaga gatttatcta gtttcttttt ttacaagaaa
     6481 aaagaaagtt cttaaaggtt ttatactttt ggtcgtagag cacacggttt aacgacttaa
     6541 ttacgaagta aataagtcta gtgtgttaga ctttaatgtt tttttaaggc attagtgcat
     6601 ttaagcgtca gagcatggct ttatgccgag aaaactattg gttggaatgg cgtgtgtgtt
     6661 agccaaagct cctgcaggat ccgaattcta gaggtacccg ggcggccgc
//
