LOCUS       40924_22794        5199 bp DNA     circular SYN 18-DEC-2018
DEFINITION  GFP fusion vector pGUG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Oka A, Sugisaki H, Takanami M.
  TITLE     Nucleotide sequence of the kanamycin resistance transposon Tn903
  JOURNAL   J. Mol. Biol. 147 (2), 217-226 (1981)
  PUBMED    6270337
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Heim R, Cubitt AB, Tsien RY.
  TITLE     Improved green fluorescence
  JOURNAL   Nature 373 (6516), 663-664 (1995)
  PUBMED    7854443
REFERENCE   3  (bases 1 to 5199)
  AUTHORS   Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P.
  TITLE     Heterologous HIS3 marker and GFP reporter modules for PCR-targeting 
            in Saccharomyces cerevisiae
  JOURNAL   Yeast 13 (11), 1065-1075 (1997)
  PUBMED    9290211
REFERENCE   4  (bases 1 to 5199)
  AUTHORS   Wendland J, Ayad-Durieux Y, Knechtle P, Rebischung C, Philippsen P.
  TITLE     PCR-based gene targeting in the filamentous fungus Ashbya gossypii
  JOURNAL   Gene 242 (1-2), 381-391 (2000)
  PUBMED    10721732
REFERENCE   5  (bases 1 to 5199)
  AUTHORS   Knechtle P, Dietrich F, Philippsen P.
  TITLE     Maximal Polar Growth Potential Depends on the Polarisome Component 
            AgSpa2 in the Filamentous Fungus Ashbya gossypii
  JOURNAL   Mol. Biol. Cell 14 (10), 4140-4154 (2003)
  PUBMED    12937275
REFERENCE   6  (bases 1 to 5199)
  AUTHORS   Knechtle P, Philippsen P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2002) Microbiology, Biozentrum, Universitaet 
            Basel, Klingelbergstrasse 70, Basel 4056, Switzerland
REFERENCE   7  (bases 1 to 5199)
  TITLE     Direct Submission
REFERENCE   8  (bases 1 to 5199)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "1981"; volume: "147"; issue: "2"; pages: "217-226"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nature"; 
            date: "1995"; volume: "373"; issue: "6516"; pages: "663-664"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Yeast"; 
            date: "1997"; volume: "13"; issue: "11"; pages: "1065-1075"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Gene 242 
            (1-2), 381-391 (2000)"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Mol. Biol. 
            Cell"; date: "2003"; volume: "14"; issue: "10"; pages: "4140-4154"
COMMENT     SGRef: number: 6; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2002) Microbiology, Biozentrum, Universitaet Basel, 
            Klingelbergstrasse 70, Basel 4056, Switzerland"
COMMENT     SGRef: number: 7; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        184..202
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    257..292
                     /label=encodes 6x Gly-Ala linker
                     /note="encodes 6x Gly-Ala linker"
     CDS             305..1015
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      1027..1268
                     /label=Saccharomyces cerevisiae URA3
                     /note="Saccharomyces cerevisiae URA3"
                     /regulatory_class="terminator"
     regulatory      1275..1881
                     /label=Saccharomyces cerevisiae TEF2
                     /note="Saccharomyces cerevisiae TEF2"
                     /regulatory_class="promoter"
     CDS             1882..2688
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      2692..2966
                     /label=Saccharomyces cerevisiae TEF2
                     /note="Saccharomyces cerevisiae TEF2"
                     /regulatory_class="terminator"
     promoter        complement(3037..3055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4075..4932)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4933..5037)
                     /label=AmpR promoter
ORIGIN
        1 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
       61 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
      121 ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
      181 acgatttagg tgacactata gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg
      241 acggatcccc ggggccggtg caggcgctgg agctggcgcc ggtgctggcg cagggttaat
      301 taacagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      361 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      421 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      481 tgtcactact ttcacttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg
      541 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      601 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      661 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      721 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      781 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      841 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      901 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      961 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataggg
     1021 cgcgccatta taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt
     1081 atatcagtta ttacccggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa
     1141 aattggaaag aaaaagcttc atggccttta taaaaaggaa ctatccaata cctcgccaga
     1201 accaagtaac agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat
     1261 ggacgcatag atctggtgta tttaccaata atgtttaatg tatatatata tatatatata
     1321 tggggccgta tacttacata tagtagatgt caagcgtagg cgcttcccct gccggctgtg
     1381 agggcgccat aaccaaggta tctatagacc gccaatcagc aaactacctc cgtacattca
     1441 tgttgcaccc acacatttat acacccagac cgcgacaaat tacccataag gttgtttgtg
     1501 acggcgtcgt acaagagaac gtgggaactt tttaggctca ccaaaaaaga aagaaaaaat
     1561 acgagttgct gacagaagcc tcaagaaaaa aaaaattctt cttcgactat gctggaggca
     1621 gagatgatcg agccggtagt taactatata tagctaaatt ggttccatca ccttcttttc
     1681 tggtgtcgct ccttctagtg ctatttctgg cttttcctat tttttttttt ccatttttct
     1741 ttctctcttt ctaatatata aattctcttg cattttctat ttttctctct atctattcta
     1801 cttgtttatt cccttcaagg ttttttttta aggagtactt gtttttagaa tatacggtca
     1861 acgaactata attaactaaa catgggtaag gaaaagactc acgtttcgag gccgcgatta
     1921 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1981 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2041 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2101 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2161 ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat
     2221 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2281 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2341 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2401 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     2461 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2521 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2581 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2641 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agagtaataa
     2701 ttattgcttc catataatat ttttatatac ctcttatttt tatgtattag ttaattaagt
     2761 atttttatct atctgcttat cattttcttt tcatataggg ggggttggtg ttttcttgcc
     2821 catcagattg atgtcctcca actcggcact attttacaaa gggttttttt gtaagagaag
     2881 gagaagacag atactaaacc atacgttact cgaaacaaaa aaaaaaaaaa tggaaaaagc
     2941 tgctatcaac aaaagacggc ctcatcgttt aaacgagctc cgtgccaggt ccctggggag
     3001 actagtggcc tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc
     3061 gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3121 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3481 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     3541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3721 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4021 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4081 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4141 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4201 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4261 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4321 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4681 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5041 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     5101 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     5161 cctctgacac atgcagctcc cggagacggt cacagcttg
//
