LOCUS       40924_22764       10439 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Ti plasmid binary vector pGSh16.0520, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Fister AS, Landherr L, Maximova SN, Guiltinan MJ.
  TITLE     Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 
            Enhances Defense Response in Theobroma cacao
  JOURNAL   Front Plant Sci 9, 268 (2018)
  PUBMED    29552023
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   Guiltinan MJ, Maximova SN, Fister A, Landherr L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2017) Plant Sciences, Penn State University, 422 
            Life Sciences Bldg, University Park, PA 16802, USA
REFERENCE   3  (bases 1 to 10439)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci 9, 268 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-SEP-2017) Plant Sciences, Penn State University, 422 Life 
            Sciences Bldg, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      531..1119
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1449..1476)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(1568..1654)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     1718..1734
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1750..1849
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1979..2000
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2015..2045
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2049..2073
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2077..2093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2114..2132
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(2154..2172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2179..2195)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2529..3200
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             3345..3410
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3417..3437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3462..7562
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7563..7610
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7649..7901
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8345..8687
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8793..9509
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    9550..9724
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(9740..9839)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(9857..9875)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9880..9896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
       61 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
      121 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
      181 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
      241 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
      301 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
      361 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
      421 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
      481 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      661 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
      721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      901 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
      961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1141 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1201 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1261 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1321 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1381 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     1441 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     1501 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     1561 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     1621 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     1681 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     1741 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
     1801 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgcggaaact
     1861 gagaccacta ggagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1921 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     1981 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2041 tgttgtgtgg aattgtgagc ggataacaat tccacacagg aaacagctat gaccatgatt
     2101 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgccaatt cgccctatag
     2161 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2221 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2281 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     2341 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2401 acttgcctag tgagacgcta gtggtctcac aataagcttg catgcctgca ggtcaacatg
     2461 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     2521 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     2581 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     2641 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     2701 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     2761 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
     2821 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtgata
     2881 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     2941 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     3001 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     3061 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     3121 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     3181 tcatttcatt tggagaggac ctcgacctca acacaacata tacaaaacaa acgaatctca
     3241 agcaatcaag cattctactt ctattgcagc aatttaaatc atttctttta aagcaaaagc
     3301 aattttctga aaattttcac catttacgaa cgatactcga gatggactat aaggaccacg
     3361 acggagacta caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa
     3421 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg
     3481 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     3541 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     3601 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     3661 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     3721 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     3781 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     3841 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     3901 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     3961 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     4021 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     4081 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     4141 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     4201 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     4261 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     4321 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     4381 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     4441 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     4501 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     4561 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     4621 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     4681 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     4741 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     4801 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     4861 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     4921 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     4981 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     5041 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     5101 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     5161 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     5221 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     5281 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     5341 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     5401 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     5461 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     5521 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     5581 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     5641 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     5701 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     5761 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     5821 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     5881 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     5941 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     6001 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     6061 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     6121 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     6181 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     6241 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     6301 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     6361 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     6421 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     6481 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     6541 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     6601 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     6661 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     6721 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     6781 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     6841 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     6901 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     6961 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     7021 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     7081 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     7141 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     7201 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     7261 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     7321 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     7381 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     7441 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     7501 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     7561 acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taaggatcct
     7621 gattgatcga tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     7681 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     7741 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     7801 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     7861 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattcc tgcagcccgg
     7921 gggatccact agttctaagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     7981 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     8041 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     8101 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     8161 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     8221 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg
     8281 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     8341 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     8401 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     8461 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     8521 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     8581 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     8641 cgcaagaccc ttcctctata taaggaagtt catttcattc ggagaggtac gtatttttac
     8701 aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt ctacaactac
     8761 atctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag gagctgttca
     8821 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     8881 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     8941 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     9001 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     9061 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     9121 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     9181 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     9241 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     9301 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     9361 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     9421 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     9481 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga attggcatgc
     9541 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     9601 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     9661 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     9721 gatcccccga attgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     9781 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     9841 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     9901 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     9961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
    10021 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
    10081 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
    10141 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
    10201 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
    10261 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
    10321 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
    10381 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgt
//
