LOCUS       40924_22744        4287 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGSC06, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Talwar D, Gupta PS, Sidharth R, Radha KA, Radhakrishnan S.
  TITLE     Novel gene cloning vectors with specific features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Talwar D, Gupta PS, Sidharth R, Radha KA, Radhakrishnan S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2008) R
REFERENCE   3  (bases 1 to 4287)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2008) R"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(460..1176)
                     /codon_start=1
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
                     /translation="MASKGEELVPGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     RBS             complement(1184..1206)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(1233..1398)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(1451..1467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1475..1491)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1499..1529)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1544..1565)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1853..2441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2608..3264
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3293..4084)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(4092..4192)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccat gcttgcatgc ctgcaggacg
      421 actctagagg atccccgggt agcagcgggg aattcattat ttgtagagct catccatgcc
      481 atgtgtaatc ccagcagcag ttacaaactc aagaaggacc atgtggtcac gcttttcgtt
      541 gggatctttc gaaagggcag attgtgtcga caggtaatgg ttgtctggta aaaggacagg
      601 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgg atccatcttc
      661 aatgttgtgg cgaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat
      721 gtatacattg tgtgagttat agttgtactc gagtttgtgt ccgagaatgt ttccatcttc
      781 tttaaaatca atacctttta actcgatacg attaacaagg gtatcacctt caaacttgac
      841 ttcagcacgc gtcttgtagt tcccgtcatc tttgaaagat atagtgcgtt cctgtacata
      901 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatccg gataacggga
      961 aaagcattga acaccataag agaaagtagt gacaagtgtt ggccatggaa caggtagttt
     1021 tccagtagtg caaataaatt taagggtaag ctttccgtat gtagcatcac cttcaccctc
     1081 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
     1141 aactccaggt accagttctt ctcctttgct agccatatgt atatctcctt cttaaagtta
     1201 aacaaaatta tttctagccc aaaaaaacgg gtatggagaa acagtagaga gttgcgataa
     1261 aaagcgtcag gtaggatccg ctaatcttat ggataaaaat gctatggcat agcaaagtgt
     1321 gacgccgtgc aaataatcaa tgtggacttt tctgccgtga ttatagacac ttttgttacg
     1381 cgtttttgtc atggctttgg tcccgctttg ttacagaatg cttttaataa gcggggaatt
     1441 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1501 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1561 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2041 tttctcaaag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2281 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2581 aaagtatata tgagtaaact agatctaatg gagaaaaaaa tcactggata taccaccgtt
     2641 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     2701 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     2761 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     2821 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     2881 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     2941 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     3001 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     3061 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     3121 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     3181 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     3241 tgcgatgagt ggcagggcgg ggcgtaagat ctgtcatgca ttctaggtac taaaacaatt
     3301 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     3361 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     3421 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     3481 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     3541 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     3601 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     3661 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     3721 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     3781 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     3841 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     3901 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     3961 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     4021 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     4081 ccatttatta tttccttcct ctaatatttt gaagcattta tcagggttat tgtctcatga
     4141 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4201 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4261 ataggcgtat cacgaggccc tttcgtc
//
