LOCUS       pGRFP.        3441 bp DNA     circular SYN 29-AUG-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pGRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    49..70
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        85..115
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             126..134
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             140..853
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     873..889
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(918..934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             953..1627
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     promoter        1673..1777
                     /label=AmpR promoter
     CDS             1778..2635
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2809..3397
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
       61 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      121 aattgtaagg aggttattta tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      181 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      241 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      301 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      361 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      421 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      481 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      541 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      601 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      661 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      721 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      781 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      841 tgaactatac aaatctggct ctggctctgg ctgtaaaacg acggccagtt ctgcggccgc
      901 ggatatctct gtcgacggtc atagctgttt cctgggttcc ggttccggtt ccatggcctc
      961 ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
     1021 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1081 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     1141 ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa
     1201 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1261 cgtggtgacc gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa
     1321 gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ctatgggctg
     1381 ggagccctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa
     1441 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc
     1501 caagaagccc gtgcagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc
     1561 ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggac gccaccatct
     1621 gttcctttag aggcctcatt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1681 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1741 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1801 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1861 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1921 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1981 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2041 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2101 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2161 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2221 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2281 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2341 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2401 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2461 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2521 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2581 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2641 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2701 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2761 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2821 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2881 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2941 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3001 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3061 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3121 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3181 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3241 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3301 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3361 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3421 acggttcctg gccttttgct c
//
