LOCUS       62056_11855        2705 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        87..121
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     495..511
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(515..571)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(580..596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(604..620)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(628..658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(673..694)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(981..1569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1743..2600)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2601..2705)
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggcttga cagctagctc agtcctaggt ataatactag
      121 ttttttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      181 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      241 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      301 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      361 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      421 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      481 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
      541 tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
      601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      721 ccagtcggga aacctgtcag ttttagagct agaaatagca agttaaaata aggctagtcc
      781 gttatcaact tgaaaaagtg gcacccttcc tcgctcactg actcgctgcg ctcggtcgtt
      841 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      961 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1021 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1081 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1141 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1201 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1261 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1321 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1381 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1441 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1501 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1561 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1621 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1681 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1741 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1801 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1861 agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc agcaataaac
     1921 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1981 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2041 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2101 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2161 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2221 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2281 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2341 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2401 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2461 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2521 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2581 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2641 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2701 ccgcg
//
