LOCUS       40924_22528       11005 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGR-PepMV-ON, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Wu X, Wang A.
  TITLE     Evaluation the resistance of tomato varieties against Pepino mosaic 
            virus with an Ontario PepMV isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Wu X, Wang A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) Agriculture and Agri-Food Canada, London 
            Research and Development Centre, 1391 Sandford Street, London, 
            Ontario N5V4T3, Canada
REFERENCE   3  (bases 1 to 11005)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) Agriculture and Agri-Food Canada, London Research and 
            Development Centre, 1391 Sandford Street, London, Ontario N5V4T3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  64..831
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      1037..1472
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    complement(1623..1647)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    1886..1907
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1922..1952
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1960..1976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1984..2000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2021..2039
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2147..2490
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5'UTR           2490..2574
                     /label=PepMV 5' UTR
                     /note="PepMV 5' UTR"
     CDS             2575..6894
                     /codon_start=1
                     /gene="PepMV RdRp"
                     /product="RNA-dependent RNA polymerase"
                     /label=PepMV RdRp
                     /protein_id="APO36649.1"
                     /translation="MSRVRNTLEKIRDPQVQSSICEAAYQHVRPVLKESLINCPYALND
                     YEADTLENLGVTINPHAIQTHTHAAAKVVENRMLEIVGHHLPKDEKVTFIFLKRSKLRY
                     MRRAAVHKDVFVNHNIEPKDFFRYDEESTSTSFSVNTRIAYISDSLHFMEPADVTHLFD
                     RCQNLKTLMATVVLPVEAIHRQTSLFPAIYSINYNEEGFEYIPGSHGGGAYFHKYETLD
                     WLKYSRFIGHDPLTGLKYTITIQMVESLGANHLFLFQRGNFETPLYRTFQKNSFVTFPN
                     IFHPRHVNATKPMPRSRAIQLYLYVKSVNKVTQRDIFAKVRQLISTAELELYDPDELTH
                     VVNYFSYVSQLSSINDYDNMLKSSFFKKLVAPMQHDWRCMIEFFRGKSDFNQLLTALQW
                     KDFSYTIKTEELIITTHTAIGQAISGAANTYKERRQLTQLVKQGVISLADFQSEEPKIE
                     YTEFERETKPPVDCVTNYNNAVKNLGLSEPVDLPECSYARNSVPNNEISMAMTDADNFA
                     VINEIESLLSEEIAAPTLPALKNKTWASYASDTSSKKNEEIENIIAELEASRKVSNVQQ
                     TQHNYHISQCPTSLTAELPWKAWLPLLNAHGFKGDQIQHSPDGQIIQPIQDINNKTPRS
                     EYPSSIPADLVSTLRNIKRAVYAIPISHRRASAYSSDVKNNRTGKLLCAQSKEWKESFA
                     FKMQHEDIVKSGVVIHGCGGSGKSQALQNFLRTLGDNNDCCTVVVPTVELRNDWVNKLC
                     KLPMEHIKTFEKAMIQPGFPVVIFDDYTKLPPGYIEAYLFHHANTELFILTGDSRQSVY
                     HESNNEAYIASLDEAVAYYSNYCGFYLNATHRNVRSLANKLGVYSEKEGHLKITFASNA
                     LQKCKVPILVPSQMKKNAMQDIGHKAMTYAGCQGLTAPRVQILLDNHTQHCSDRVLYTC
                     LSRAVDSIHFINTGPNNSEFWDKLEATPYLKAFIDTYRDEKTEMLNSKPADDSPVEPEA
                     PATHFPVSNGNNLEKLASTLPEKFAREIYDKHHGYSNTIQTENPIVQLFQHQQAKDETL
                     FWATIEARLSITTPDANLREFTLKKDVGDILFFNYHSAMCLPADPVDFEPRTWEICAAE
                     VKNTYLAKPMANLINAASRQSPDFEPNKISLFLKSQWVKKVEKLGAIKSKPGQTIAAFM
                     QQTVMLYGTMARYLRKMRQRFQPKHIFINCETTTDDLNNFVLNGWNFNRTAQTNDFTAF
                     DQSQDGAMLQFEVMKAKFFNIPADVIEGYINIKLNAKIFLGTLSIMRLSGEGPTFDANT
                     ECSIAYTATRYHLSSAVKQVYAGDDMALDGVVMEKPSFKKLQSKLKLTSKTLFPKQVKG
                     DYAEFCGWTFTPGGIIKNPLKMHASIMLQEAIGNLHTAARSYAIDMKHSYQMGDKLHEY
                     LTPDEAEQHFLAVRKLHKLHQGEAMRLGEKSPPKATH"
     gene            2575..6894
                     /gene="PepMV RdRp"
                     /label=PepMV RdRp
                     /note="PepMVgp1"
     CDS             6919..7623
                     /codon_start=1
                     /gene="PepMV TGB1"
                     /product="TGBp1"
                     /label=PepMV TGB1
                     /note="movement protein"
                     /protein_id="APO36650.1"
                     /translation="MERSTLINLLQLHHFEPKLSVEGVIVVHGIAGTGKTTLLRTLFSA
                     YPNLVIGSPRPCYLDKANKISQVCLSCFPNTLCDIVDEYHLLESYQEPTLALFGDPCQC
                     TFIGRLRIPHYTSFRTHRFGKSTAELLNKLFQLQIVSVKQEDDIVEFFDPFQVDPTENI
                     SASEEEVLEFVSDQVVTTSSEELAGLEFTETTFYCTTLAAAVTENPARTFISLTRHTQR
                     LTIGELNARIDS"
     gene            6919..7623
                     /gene="PepMV TGB1"
                     /label=PepMV TGB1
                     /note="PepMVgp2"
     CDS             7604..7975
                     /codon_start=1
                     /gene="PepMV TGB2"
                     /product="TGBp2"
                     /label=PepMV TGB2
                     /note="movement protein"
                     /protein_id="APO36652.1"
                     /translation="MPGLTPRADLTDTYKIIAIAFLLSACIYFQNSHYQPVAGDNLHRL
                     PFGGQYQDGTKKISYFPQQQSYFHSGNKLNVLILIFILTLGIVLTNKFSFSVSRTTHQH
                     SCYNTHSAANTTPPLSGHH"
     gene            7604..7975
                     /gene="PepMV TGB2"
                     /label=PepMV TGB2
                     /note="PepMVgp3"
     CDS             7827..8075
                     /codon_start=1
                     /gene="PepMV TGB3"
                     /product="TGBp3"
                     /label=PepMV TGB3
                     /note="movement protein"
                     /protein_id="APO36653.1"
                     /translation="MSSYSSSFLHWVSSSPINLVLALAVLLTSILATTHILQQTQHHHC
                     QVIIDGAAIVVTNCENTPEVLKAINFAPWNGLSFPNV"
     gene            7827..8075
                     /gene="PepMV TGB3"
                     /label=PepMV TGB3
                     /note="PepMVgp4"
     CDS             8122..8835
                     /codon_start=1
                     /gene="PepMV CP"
                     /product="CP"
                     /label=PepMV CP
                     /note="coat protein"
                     /protein_id="APO36651.1"
                     /translation="MADNTPVAATSGSPPTAQDAGARAPADFSNPNTAPSLSDLKKVKY
                     VSTVTSVATPTEIEALGKIFTAMGLAANETGPAMWDLARAYADVQSSKSAQLIGATPSN
                     PALSRRALAAQFDRINITPRQFCMYFAKVVWNILLDSNIPPANWAKLGYQEDTKFAAFD
                     FFDGVTNPASLQPADGLIRQPNEKELAAHSVAKYGALARQKISTGNYITTLGEVTRGHM
                     GGANTMYAIDAPPEL"
     gene            8122..8835
                     /gene="PepMV CP"
                     /label=PepMV CP
                     /note="PepMVgp5"
     3'UTR           8836..8904
                     /label=PepMV 3' UTR
                     /note="PepMV 3' UTR"
     terminator      8949..9201
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9210..9226)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(9382..9404)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(9471..10059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10163..10975)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
       61 ttgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
      121 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
      181 caaaagcacc gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact
      241 gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat
      301 gaatggcgtt ggatgccggg caacagcccg cattatgggc gttggcctca acacgatttt
      361 acgtcactta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac
      421 gtcatcgtct gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag
      481 cgctggctgt tttacgcgta tgacagtctc cggaagacgg ttgttgcgca cgtattcggt
      541 gaacgcacta tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg
      601 atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta
      661 atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg
      721 gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc
      781 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac cagattttga
      841 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
      901 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
      961 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
     1021 gcgattcagg cgatccccat ccaacagccc gccgtcgagc gggctttttt atccccggaa
     1081 gcctgtggat agagggtagt tatccacgtg aaaccgctaa tgccccgcaa agccttgatt
     1141 cacggggctt tccggcccgc tccaaaaact atccacgtga aatcgctaat cagggtacgt
     1201 gaaatcgcta atcggagtac gtgaaatcgc taataaggtc acgtgaaatc gctaatcaaa
     1261 aaggcacgtg agaacgctaa tagccctttc agatcaacag cttgcaaaca cccctcgctc
     1321 cggcaagtag ttacagcaag tagtatgttc aattagcttt tcaattatga atatatatat
     1381 caattattgg tcgcccttgg cttgtggaca atgcgctacg cgcaccggct ccgcccgtgg
     1441 acaaccgcaa gcggttgccc accgtcgagc gcctttgccc acaacccggc ggccggccgc
     1501 aacagatcgt tttataaatt tttttttttg aaaaagaaaa agcccgaaag gcggcaacct
     1561 ctcgggcttc tggatttccg atccccggaa ttagatctca aacaaacaca tacagcgact
     1621 tagtttaccc gccaatatat cctgtcaagg ccttcatgtt ctttcctgcg ttatcccctg
     1681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1741 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     1801 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1861 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     1921 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     1981 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     2041 aacaaaagct ggagctcgca tgcctgcagg tcaacatggt ggagcacgac acacttgtct
     2101 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
     2161 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
     2221 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     2281 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     2341 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     2401 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     2461 tataaggaag ttcatttcat ttggagagga gaaaacaaaa cataaccaaa acaacaaagt
     2521 cataataaca ctacataacc acgcggaaaa acagctaacg cactttaccg cattatgtct
     2581 cgtgttagaa atactttgga aaagatcaga gaccctcaag tacagtcaag catatgcgaa
     2641 gcagcatacc aacatgttcg accagtttta aaggaatctc taatcaattg tccctatgca
     2701 ctcaatgatt atgaagccga cacccttgag aatttaggtg tcacaattaa ccctcatgca
     2761 attcaaaccc atacacacgc tgcagctaaa gtggttgaaa acagaatgct agagattgtt
     2821 ggtcaccatc taccaaaaga tgagaaggtt accttcattt tcctaaaacg tagtaaattg
     2881 cgttacatga gaagagctgc tgttcataaa gatgtttttg tcaaccataa catagagccc
     2941 aaagattttt ttaggtatga cgaggagtcc acatcaacaa gtttctccgt gaatactaga
     3001 atcgcataca tttcagattc cttacatttc atggagccag ctgatgttac acaccttttt
     3061 gaccgttgcc aaaatcttaa aacattaatg gcaaccgttg tcctacctgt cgaagcaatt
     3121 cataggcaaa catctttatt tcctgcgata tactccatta actacaatga agaaggcttt
     3181 gagtatatcc ctggttccca tggtggtggg gcatactttc acaagtatga gaccttagac
     3241 tggcttaaat attccagatt cataggacat gatccattga ctggcctcaa atataccatt
     3301 acaattcaaa tggttgaaag tctaggtgcc aaccaccttt tccttttcca aagaggaaat
     3361 tttgaaacgc ccttatatag gacctttcaa aaaaatagtt ttgtgacatt ccctaatata
     3421 tttcatcccc gacatgttaa tgccacaaaa cctatgccac gctccagggc aatccaattg
     3481 tacttatatg ttaagtctgt caacaaagtc acacaaagag acatctttgc aaaagttaga
     3541 cagcttatat ccacagctga gctggaactg tatgaccctg atgagcttac tcatgttgtc
     3601 aattactttt catatgtttc acaattgtca tcgataaatg attatgacaa catgctaaaa
     3661 tccagtttct tcaagaaatt ggttgcaccc atgcaacacg actggaggtg catgattgaa
     3721 ttcttccggg gaaagagtga tttcaatcaa cttctcacag ctcttcaatg gaaagatttt
     3781 tcctacacta tcaaaactga agagctaata attactacac acactgccat tgggcaagct
     3841 ataagtggag cagctaatac ttataaggaa agaaggcaat taacacaact ggtcaagcaa
     3901 ggagtaattt cactcgctga ttttcaatct gaagagccca aaatagaata caccgaattc
     3961 gagcgagaaa caaaaccacc agttgattgt gtgactaatt acaacaatgc tgttaagaac
     4021 cttggcctgt ctgaaccagt ggatctgcca gaatgctcat acgctagaaa ctctgttcct
     4081 aacaatgaga tatctatggc tatgactgat gctgacaact tcgctgttat aaatgaaatt
     4141 gaatctctct tgtctgaaga aatcgcagca ccaacacttc cagcattgaa aaataaaaca
     4201 tgggctagtt atgcatctga tacttcatca aagaagaatg aagaaattga aaatataatt
     4261 gctgagctag aagcttcacg taaggtctcc aatgtccagc aaacacagca caactaccat
     4321 atctcacaat gccccacttc cttaactgct gagcttccat ggaaagcctg gctcccactt
     4381 ctcaatgccc atggtttcaa aggagatcaa atccaacata gtcccgacgg ccaaataatc
     4441 caaccaattc aggacatcaa caataaaaca ccaagatctg aatacccatc tagcatccct
     4501 gcagatcttg tttcaacttt gcgcaatatt aaaagagcag tttatgctat tcctatcagc
     4561 cacagaaggg ccagcgctta tagctcagat gttaagaata acagaactgg aaaactgctc
     4621 tgtgctcaat ctaaagaatg gaaagagagt tttgctttca aaatgcaaca tgaagacata
     4681 gtcaaatctg gtgttgtcat acatggttgt ggcggctccg gaaaatcaca agcattgcaa
     4741 aacttcctta gaactttggg tgacaataat gactgctgta cagttgttgt ccccacagta
     4801 gagctcagga atgactgggt taacaaattg tgcaagttgc ctatggaaca catcaaaaca
     4861 tttgaaaaag caatgattca accaggcttt cctgttgtca tttttgacga ctatactaag
     4921 ctgccacccg gttacattga agcttatctg ttccatcacg ccaacactga actcttcatc
     4981 ctcactggtg actctagaca aagtgtatat catgagtcca acaatgaagc ttacattgcc
     5041 tctcttgatg aagctgttgc ctactattcc aactactgtg gtttctattt gaacgcgacg
     5101 cacagaaatg tacgtagttt agctaacaag ttgggagtgt acagtgaaaa agaaggacac
     5161 ctcaaaatta cttttgcatc caatgctcta caaaaatgca aagtacccat cttggtgccc
     5221 tctcagatga agaaaaatgc aatgcaagac attggccata aagcaatgac ttatgcagga
     5281 tgtcaaggac ttactgctcc aagagttcaa attttgcttg acaaccacac acagcattgc
     5341 tcggacagag ttctttacac atgtctttca cgagctgtgg attctatcca ttttatcaat
     5401 actggcccca acaattctga attttgggat aagcttgaag ctacaccata cctcaaagcc
     5461 tttattgata cataccggga tgaaaagact gagatgttga attcaaaacc tgcagatgac
     5521 agccctgtgg aacctgaagc tcctgctact catttccccg tctctaacgg caacaactta
     5581 gagaaattag cttcaacact acctgaaaag tttgctcggg agatctatga taaacatcat
     5641 ggttattcca acaccataca aacagagaac cccatagtac aacttttcca acatcaacag
     5701 gcaaaagatg aaactctctt ttgggcaaca attgaggcaa gactttctat aaccacacca
     5761 gacgctaacc tcagagaatt tactttaaag aaagacgttg gagatattct gttctttaac
     5821 tatcactcag caatgtgcct gccagctgac cctgtcgact tcgagccaag aacttgggaa
     5881 atatgtgcag ctgaagtcaa gaatacatac ctagcaaaac caatggctaa tttgataaat
     5941 gccgctagtc gacaatctcc tgacttcgag cctaacaaga tctcactgtt tttgaagtca
     6001 caatgggtta agaaagtaga gaaattggga gccatcaaat caaagcctgg gcaaacaatt
     6061 gcggctttta tgcagcaaac cgttatgctg tacggaacta tggctagata cttaagaaaa
     6121 atgagacaaa gattccaacc aaaacacatt ttcatcaatt gtgaaactac aaccgatgat
     6181 ctgaataatt ttgttctcaa tggttggaat tttaatcgaa cagctcagac taatgatttc
     6241 actgcatttg atcaatccca agatggagct atgttacaat ttgaagtcat gaaggctaaa
     6301 ttcttcaaca ttcctgcaga cgtgattgaa gggtatatca acattaagct taacgctaaa
     6361 atcttcctag gcactctttc aattatgagg ttatcaggtg aaggtcccac atttgatgca
     6421 aacactgaat gctctattgc ttacacagca acaagatacc atctgagctc agcagtgaaa
     6481 caagtttacg ctggtgatga catggctctt gacggagtgg tcatggaaaa accctccttt
     6541 aagaaattac agagcaagct taaattgaca tcaaaaacat tgtttccaaa acaagttaag
     6601 ggtgattatg ccgaattttg tgggtggact tttaccccag gtgggattat taaaaatcct
     6661 ttgaaaatgc atgcatccat catgttacaa gaagcaatag gtaacctcca cactgctgct
     6721 aggtcatatg caatagacat gaagcattca taccaaatgg gagataaatt gcatgaatac
     6781 cttactcctg atgaagctga gcaacatttc ttggctgttc gcaaattgca caaactccac
     6841 caaggtgaag ccatgagact tggggagaag agccccccaa aagcaacaca ttgacgggtt
     6901 aagtttcccc tgttcgaaat ggaaagatcc actctaataa atttacttca actgcaccac
     6961 tttgaaccta aactcagcgt tgaaggagtt attgttgtac acggaatcgc tggaacaggg
     7021 aaaactaccc tacttagaac tttattttct gcttacccga acctagttat aggatcacct
     7081 aggccttgtt acttagataa agctaataaa atttcacaag tttgtttatc ttgctttcca
     7141 aacacccttt gtgatattgt cgatgagtac catctcctag aaagttacca agaacctact
     7201 ttggctttgt ttggagaccc ttgtcagtgt actttcattg gaagacttag aattccacac
     7261 tacacttcct tcaggacgca tagattcggc aagtctactg cagagctctt aaacaagttg
     7321 tttcaacttc aaattgtctc tgtaaaacaa gaagacgaca tagttgaatt ctttgaccca
     7381 tttcaagtag acccaactga aaatatttca gcatctgagg aagaagtttt ggaatttgtc
     7441 tctgatcaag tagtgacgac cagctctgaa gaattagcag gattggagtt caccgaaacc
     7501 actttctact gcactacact agctgcagca gtcactgaaa atcctgccag aactttcatc
     7561 tccttaacca gacacactca gagactcacc attggtgaac taaatgccag gattgactcc
     7621 tagagctgac ctcactgaca cctacaagat aattgctatt gcctttttgt tgtcagcttg
     7681 catttacttc caaaacagcc attaccaacc tgttgctgga gataatctgc atagattgcc
     7741 atttggtggc cagtatcaag acggcaccaa gaagatatca tattttcctc agcaacaatc
     7801 atactttcac tctggtaaca aattaaatgt cctcatactc atcttcattc ttacattggg
     7861 tatcgtcctc accaataaat ttagttttag cgttagccgt actactcacc agcattcttg
     7921 ctacaacaca cattctgcag caaacacaac accaccattg tcaggtcatc attgacggag
     7981 ctgccatagt tgttactaat tgtgaaaaca cgcctgaagt tctaaaagca atcaacttcg
     8041 ccccttggaa cgggttaagt tttcctaatg tttgaaaatt aactttgagc acttcacaat
     8101 taagctaaca attcactaat catggctgac aataccccag ttgctgctac ttctggttcc
     8161 cctccaactg ctcaagatgc tggtgccaga gcccctgctg acttttcaaa tcctaataca
     8221 gctcctagcc tcagtgattt gaagaaagtc aagtatgtgt ccacagtcac ttcagtggca
     8281 acaccaactg aaatagaagc cctcggaaag atcttcaccg ccatgggact ggctgctaat
     8341 gaaactggac ccgctatgtg ggatctagcg cgtgcttatg ctgatgtcca aagttcaaaa
     8401 tccgcacaac tcataggtgc caccccctca aatccagcac tctcacgccg cgcacttgct
     8461 gcccaatttg atcgtattaa catcacaccc aggcagtttt gcatgtattt tgctaaagtt
     8521 gtctggaaca ttctgctaga cagcaatatt ccaccagcaa actgggccaa gcttggttac
     8581 caagaagaca caaaatttgc tgctttcgat ttcttcgatg gagtcaccaa tccagcaagt
     8641 ttgcaaccag ctgacggcct catccggcaa cccaatgaaa aggagcttgc tgctcactca
     8701 gttgctaagt atggtgcact agccaggcag aaaatttcca ctggcaacta cataaccaca
     8761 cttggagagg tcacacgtgg acacatgggt ggagctaaca ccatgtacgc aattgatgca
     8821 cccccagaac tttaaaacac tcgaaactta attagagtgg ggttttctat agtttatttt
     8881 cccaataaat tgcttttgta atttaaaaaa aaaaaaaaaa aaaactagtg ggtaccgcga
     8941 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     9001 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     9061 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     9121 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     9181 tgtcatctat gttactagat cgggaattca ctggccgtcg ttttacaacg tcgtgactgg
     9241 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9301 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9361 gaatggcgcc tgatgagaac gttacaccac aatatatcct gccaagatct catgtgagca
     9421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catggttaca
    10141 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
    10201 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
    10261 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
    10321 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
    10381 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
    10441 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
    10501 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
    10561 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
    10621 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
    10681 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
    10741 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
    10801 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg
    10861 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
    10921 tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac
    10981 cccttgtatt actgtttatg taagc
//
