LOCUS       40924_22503        9133 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGPro3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Matsumoto TK, Keith LM, Cabos RY, Suzuki JY, Gonsalves D, Thilmony 
            R.
  TITLE     Screening promoters for Anthurium transformation using transient 
            expression
  JOURNAL   Plant Cell Rep. 32 (3), 443-451 (2013)
  PUBMED    23283558
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2011) Crop Improvement and Utilization research 
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 9133)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2013"; volume: "32"; issue: "3"; pages: "443-451"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2011) Crop Improvement and Utilization research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    264..285
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        300..330
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    338..354
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     362..378
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        399..417
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    430..537
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     CDS             558..2363
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2388..3104
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3114..3131
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      3163..3415
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3425..3458
                     /label=loxP attachment site for Cre recombinase
                     /note="loxP attachment site for Cre recombinase"
     protein_bind    3425..3458
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        3465..5174
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin
                     gene RUBQ2"
     CDS             5195..6217
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    complement(6252..6285)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6300..6474
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(6552..6576)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(6586..6608)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(6743..7178)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             7469..8281
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      8455..9043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaggcc ttcatgttct ttcctgcgtt atcccctgat
       61 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      121 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      181 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      241 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      301 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      361 acaggaaaca gctatgacca tgattacgcc aagctcggaa ttaaccctca ctaaagggaa
      421 caaaagctgg agctccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg
      481 caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccca
      541 tggtagatct gactagttta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
      601 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
      661 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
      721 ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac
      781 cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
      841 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
      901 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
      961 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga
     1021 aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc
     1081 tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag
     1141 actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac
     1201 tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag
     1261 tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     1321 ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag
     1381 tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     1441 atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg
     1501 cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag
     1561 agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg
     1621 gctttcagct gtctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     1681 gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga
     1741 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     1801 cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     1861 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     1921 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     1981 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     2041 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     2101 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     2161 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     2221 cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca
     2281 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     2341 aaccgcagca gggaggcaaa caagctagcg gagcaggtgg cgctggtatg gtgagcaagg
     2401 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2461 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2521 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2581 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2641 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2701 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2761 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2821 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2881 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2941 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3001 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3061 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagct agccaccacc
     3121 accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     3181 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     3241 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     3301 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     3361 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcggggg
     3421 taccataact tcgtatagca tacattatac gaagttatgc atgcattcgg gtcaaggcgg
     3481 aagccagcgc gccaccccac gtcagcaaat acggaggcgc ggggttgacg gcgtcacccg
     3541 gtcctaacgg cgaccaacaa accagccaga agaaattaca gtaaaaaaaa agtaaattgc
     3601 actttgatcc accttttatt acctaagtct caatttggat cacccttaaa cctatctttt
     3661 caatttgggc cgggttgtgg tttggactac catgaacaac ttttcgtcat gtctaacttc
     3721 cctttcagca aacatatgaa ccatatatag aggagatcgg ccgtatacta gagctgatgt
     3781 gtttaaggtc gttgattgca cgagaaaaaa aaatccaaat cgcaacaata gcaaatttat
     3841 ctggttcaaa gtgaaaagat atgtttaaag gtagtccaaa gtaaaactta tagataataa
     3901 aatgtggtcc aaagcgtaat tcactcaaaa aaaatcaacg agacgtgtac caaacggaga
     3961 caaacggcat cttctcgaaa tttcccaacc gctcgctcgc ccgcctcgtc ttcccggaaa
     4021 ccgcggtggt ttcagcgtgg cggattctcc aagcagacgg agacgtcacg gcacgggact
     4081 cctcccacca cccaaccgcc ataaatacca gccccctcat ctcctctcct cgcatcagct
     4141 ccacccccga aaaatttctc cccaatctcg cgaggctctc gtcgtcgaat cgaatcctct
     4201 cgcgtcctca aggtacgctg cttctcctct cctcgcttcg tttcgattcg atttcggacg
     4261 ggtgaggttg ttttgttgct agatccgatt ggtggttagg gttgtcgatg tgattatcgt
     4321 gagatgttta ggggttgtag atctgatggt tgtgatttgg gcacggttgg ttcgataggt
     4381 ggaatcgtgg ttaggttttg ggattggatg ttggttctga tgattggggg gaatttttac
     4441 ggttagatga attgttggat gattcgattg gggaaatcgg tgtagatctg ttggggaatt
     4501 gtggaactag tcatgcctga gtgattggtg cgatttgtag cgtgttccat cttgtaggcc
     4561 ttgttgcgag catgttcaga tctactgttc cgctcttgat tgagttattg gtgccttggg
     4621 ttggtgcaaa cacaggcttt aatatgttat atctgttttg tgtttgatgt agatctgtag
     4681 ggtagttctt cttagacatg gttcaattat gtagcttgtg cgtttcgatt tgatttcata
     4741 tgttcacaga ttagataatg atgaactctt ttaattaatt gtcaatggta aataggaagt
     4801 cttgtcgcta tatctgtcat aatgatctca tgttactatc tgccagtaat ttatgctaag
     4861 aactatatta gaatatcatg ttacaatctg tagtaatatc atgttacaat ctgtagttca
     4921 tctatataat ctattgtggt aatttctttt tactatctgt gtgaagatta ttgccactag
     4981 ttcattctac ttatttctga agttcaggat acgtgtgctg ttactaccta tctgaataca
     5041 tgtgtgatgt gcctgttact atctttttga atacatgtat gttctgttgg aatatgtttg
     5101 ctgtttgatc cgttgttgtg tccttaatct tgtgctagtt cttaccctat ctgtttggtg
     5161 attatttctt gcagattcag atcgctagcc caccatgaaa aagcctgaac tcaccgcgac
     5221 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     5281 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     5341 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     5401 ggccgcgctc ccgattccgg aagtgcttga cattggggag tttagcgaga gcctgaccta
     5461 ttgcatctcc cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     5521 cgctgttcta caaccggtcg cggaggctat ggatgcgatc gctgcggccg atcttagcca
     5581 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     5641 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     5701 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     5761 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     5821 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     5881 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     5941 cgagcggagg catccggagc ttgcaggatc gccacgactc cgggcgtata tgctccgcat
     6001 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     6061 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     6121 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     6181 aaaccgacgc cccagcactc gtccgagggc aaagaaatag agtagatgcc gaccggatct
     6241 gtcgaactag cataacttcg tatagcatac attatacgaa gttattcgac aagctcgagt
     6301 ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt
     6361 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     6421 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga
     6481 attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     6541 agcgtcaatt tgtttacacc acaatatatc ctgccaccgt taacgttaca ccacaatata
     6601 tcctgccaag atctctaatt ccggggatcg gaaatccaga agcccgagag gttgccgcct
     6661 ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac gatctgttgc ggccggccgc
     6721 cgggttgtgg gcaaaggcgc tggcgctcga cggtgggcaa ccgcttgcgg ttgtccacgg
     6781 gcggagccgg tgcgcgtagc gcattgtcca caagccaagg gcgaccaata attgatatat
     6841 atattcataa ttgaaaagct aattgaacat actacttgct gtaactactt gccggagcga
     6901 ggggtgtttg caagctgttg atctgaaagg gctattagcg ttctcacgtg cctttttgat
     6961 tagcgatttc acgtgacctt attagcgatt tcacgtactc cgattagcga tttcacgtac
     7021 cctgattagc gatttcacgt ggatagtttt tggagcgggc cggaaagccc cgtgaatcaa
     7081 ggctttgcgg ggcattagcg gtttcacgtg gataactacc ctctatccac aggcttccgg
     7141 ggataaaaaa gcccgctcga cggcgggctg ttggatgggg atcgcctgaa tcgccccatc
     7201 atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt
     7261 ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat
     7321 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa
     7381 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     7441 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
     7501 aaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     7561 taatgtcggg caatcaggtg cgacaatcta ccgattgtat gggaagcccg atgcgccaga
     7621 gttgtttctg aaacatggca aaggtagcgt tgccaatgtt gttacagatg agatggtcag
     7681 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     7741 tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga
     7801 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     7861 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     7921 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     7981 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     8041 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     8101 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     8161 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     8221 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     8281 ctaatcacta gaccaatgtt acacatatat actttagatt gatttaaaac ttcattttta
     8341 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8461 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8521 ggtttgtttg ccggatcaag agctaccaac tcttcttccg aaggtaactg gcttcagcag
     8581 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8641 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8701 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8761 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8821 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8881 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8941 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9001 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9061 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgagatctc aaacaaacac
     9121 atacagcgac tta
//
