LOCUS       40924_22498        8809 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGPro2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Thilmony R, Guttman M, Chiniquy D, Blechl A.
  TITLE     pGPro1, A Novel Binary Vector for Monocot Promoter Characterization
  JOURNAL   Plant Mol. Biol. Rep. 24, 57-69 (2006)
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Thilmony R, Guttman M, Chiniquy D, Blechl A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2007) Crop Improvement and Utilization Research 
            Unit, USDA-ARS Western Regional Research Center, 800 Buchanan 
            Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8809)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol. Rep. 24, 57-69 (2006)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-SEP-2007) Crop Improvement and Utilization Research Unit, 
            USDA-ARS Western Regional Research Center, 800 Buchanan Street, 
            Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             27..1832
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             1857..2573
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2583..2600
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2632..2884
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2894..2927
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        2933..3772
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             3775..3792
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4340..5362
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    complement(5397..5430)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5445..5619
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5697..5721)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(5731..5753)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(5888..6323)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             6614..7426
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7600..8188
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8279..8303)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    8542..8563
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8578..8608
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8616..8632
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8640..8656
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8677..8695
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8708..8809
                     /label=MCS
                     /note="pBluescript multiple cloning site"
ORIGIN
        1 ggtaccccat ggtagatctg actagtttac gtcctgtaga aaccccaacc cgtgaaatca
       61 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
      121 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
      181 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
      241 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
      301 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
      361 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
      421 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
      481 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
      541 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
      601 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
      661 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
      721 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
      781 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
      841 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
      901 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
      961 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     1021 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     1081 ctgctgtcgg ctttcagctg tctttaggca ttggtttcga agcgggcaac aagccgaaag
     1141 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     1201 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     1261 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     1321 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     1381 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
     1441 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
     1501 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
     1561 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     1621 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     1681 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
     1741 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
     1801 tcggtgaaaa accgcagcag ggaggcaaac aagctagcgg agcaggtggc gctggtatgg
     1861 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1921 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1981 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2041 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2101 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2161 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2221 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2281 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2341 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2401 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2461 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2521 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcta
     2581 gccaccacca ccaccaccac gtgtgaattg gtgaccagct cgaatttccc cgatcgttca
     2641 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     2701 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     2761 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     2821 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     2881 gatcgggggt accataactt cgtatagcat acattatacg aagttatgca tgcggtcatt
     2941 catatgcttg agaagagagt cgggatagtc caaaataaaa caaaggtaag attacctggt
     3001 caaaagtgaa aacatcagtt aaaaggtggt ataaagtaaa atatcggtaa taaaaggtgg
     3061 cccaaagtga aatttactct tttctactat tataaaaatt gaggatgttt ttgtcggtac
     3121 tttgatacgt catttttgta tgaattggtt tttaagttta ttcgcttttg gaaatgcata
     3181 tctgtatttg agtcgggttt taagttcgtt tgcttttgta aatacagagg gatttgtata
     3241 agaaatatct ttaaaaaaac ctatatgcta atttgacata atttttgaga aaaatatata
     3301 ttcaggcgaa ttctcacaat gaacaataat aagattaaaa tagctttccc ccgttgcagc
     3361 gcatgggtat tttttctagt aaaaataaaa gataaactta gactcaaaac atttacaaaa
     3421 acaaccccta aagttcctaa agcccaaagt gctatccacg atccatagca agcccagccc
     3481 aacccaaccc aacccaaccc accccagtcc agccaactgg acaatagtct ccacaccccc
     3541 ccactatcac cgtgagttgt ccgcacgcac cgcacgtctc gcagccaaaa aaaaaaaaag
     3601 aaagaaaaaa aagaaaaaga aaaaacagca ggtgggtccg ggtcgtgggg gccggaaacg
     3661 cgaggaggat cgcgagccag cgacgaggcc ggccctccct ccgcttccaa agaaacgccc
     3721 cccatcgcca ctatatacat acccccccct ctcctcccat ccccccaacc ctaccaccac
     3781 caccaccacc acctccacct cctcccccct cgctgccgga cgacgagctc ctcccccctc
     3841 cccctccgcc gccgccgcgc cggtaaccac cccgcccctc tcctctttct ttctccgttt
     3901 tttttttccg tctcggtctc gatctttggc cttggtagtt tgggtgggcg agaggcggct
     3961 tcgtgcgcgc ccagatcggt gcgcgggagg ggcgggatct cgcggctggg gctctcgccg
     4021 gcgtggatcc ggcccggatc tcgcggggaa tggggctctc ggatgtagat ctgcgatccg
     4081 ccgttgttgg gggagatgat ggggggttta aaatttccgc catgctaaac aagatcagga
     4141 agaggggaaa agggcactat ggtttatatt tttatatatt tctgctgctt cgtcaggctt
     4201 agatgtgcta gatctttctt tcttcttttt gtgggtagaa tttgaatccc tcagcattgt
     4261 tcatcggtag tttttctttt catgatttgt gacaaatgca gcctcgtgcg gagctttttt
     4321 gtaggtaggc tagcccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     4381 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     4441 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     4501 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     4561 tccggaagtg cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg
     4621 ttcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc
     4681 ggtcgcggag gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     4741 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     4801 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     4861 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     4921 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     4981 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     5041 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     5101 ggagcttgca ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact
     5161 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     5221 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     5281 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     5341 cactcgtccg agggcaaaga aatagagtag atgccgaccg gatctgtcga actagcataa
     5401 cttcgtatag catacattat acgaagttat tcgacaagct cgagtttctc cataataatg
     5461 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     5521 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     5581 taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta
     5641 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     5701 acaccacaat atatcctgcc accgttaacg ttacaccaca atatatcctg ccaagatctc
     5761 taattccggg gatcggaaat ccagaagccc gagaggttgc cgcctttcgg gctttttctt
     5821 tttcaaaaaa aaaaatttat aaaacgatct gttgcggccg gccgccgggt tgtgggcaaa
     5881 ggcgctggcg ctcgacggtg ggcaaccgct tgcggttgtc cacgggcgga gccggtgcgc
     5941 gtagcgcatt gtccacaagc caagggcgac caataattga tatatatatt cataattgaa
     6001 aagctaattg aacatactac ttgctgtaac tacttgccgg agcgaggggt gtttgcaagc
     6061 tgttgatctg aaagggctat tagcgttctc acgtgccttt ttgattagcg atttcacgtg
     6121 accttattag cgatttcacg tactccgatt agcgatttca cgtaccctga ttagcgattt
     6181 cacgtggata gtttttggag cgggccggaa agccccgtga atcaaggctt tgcggggcat
     6241 tagcggtttc acgtggataa ctaccctcta tccacaggct tccggggata aaaaagcccg
     6301 ctcgacggcg ggctgttgga tggggatcgc ctgaatcgcc ccatcatcca gccagaaagt
     6361 gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt
     6421 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc
     6481 agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat
     6541 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     6601 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcaaggc cgcgattaaa
     6661 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     6721 aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     6781 tggcaaaggt agcgttgcca atgttgttac agatgagatg gtcagactaa actggctgac
     6841 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     6901 actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc
     6961 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     7021 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     7081 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     7141 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     7201 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     7261 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     7321 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     7381 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cactagacca
     7441 atgttacaca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7681 tcaagagcta ccaactcttc ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8221 ggccttttgc tggccttttg ctcacatgag atctcaaaca aacacataca gcgacttagt
     8281 ttacccgcca atatatcctg tcaaggcctt catgttcttt cctgcgttat cccctgattc
     8341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8401 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8461 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8521 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8581 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8641 aggaaacagc tatgaccatg attacgccaa gctcggaatt aaccctcact aaagggaaca
     8701 aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca
     8761 ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggccc
//
