LOCUS       40924_22493        8833 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGPro1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Thilmony R, Guttman M, Chiniquy D, Blechl A.
  TITLE     pGPro1, A Novel Binary Vector for Monocot Promoter Characterization
  JOURNAL   Plant Mol. Biol. Rep. 24 (1), 57-69 (2006)
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Thilmony R, Guttman M, Chiniquy D, Blechl A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2006) Crop Improvement and Utilization Research 
            Unit, USDA-ARS Western Regional Research Center, 800 Buchanan 
            Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8833)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol. Rep."; date: "2006"; volume: "24"; issue: "1"; pages: "57-69"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAR-2006) Crop Improvement and Utilization Research Unit, 
            USDA-ARS Western Regional Research Center, 800 Buchanan Street, 
            Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             27..1832
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             1857..2573
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2583..2600
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2632..2884
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2894..2927
                     /label=loxP attachment site for the Cre recombinase
                     /note="loxP attachment site for the Cre recombinase"
     protein_bind    2894..2927
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        2933..3772
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             3775..3792
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4365..5387
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    complement(5422..5455)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5470..5644
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5722..5746)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(5756..5778)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(5912..6347)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             6638..7450
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7624..8212
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8303..8327)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    8566..8587
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8602..8632
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8640..8656
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8664..8680
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8701..8719
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8732..8833
                     /label=MCS
                     /note="pBluescript multiple cloning site"
ORIGIN
        1 ggtaccccat ggtagatctg actagtttac gtcctgtaga aaccccaacc cgtgaaatca
       61 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
      121 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
      181 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
      241 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
      301 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
      361 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
      421 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
      481 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
      541 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
      601 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
      661 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
      721 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
      781 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
      841 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
      901 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
      961 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     1021 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     1081 ctgctgtcgg ctttcagctg tctttaggca ttggtttcga agcgggcaac aagccgaaag
     1141 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     1201 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     1261 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     1321 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     1381 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
     1441 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
     1501 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
     1561 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     1621 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     1681 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
     1741 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
     1801 tcggtgaaaa accgcagcag ggaggcaaac aagctagcgg agcaggtggc gctggtatgg
     1861 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1921 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1981 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2041 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2101 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2161 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2221 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2281 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2341 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2401 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2461 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2521 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcta
     2581 gccaccacca ccaccaccac gtgtgaattg gtgaccagct cgaatttccc cgatcgttca
     2641 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     2701 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     2761 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     2821 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     2881 gatcgggggt accataactt cgtatagcat acattatacg aagttatgca tgcggtcatt
     2941 catatgcttg agaagagagt cgggatagtc caaaataaaa caaaggtaag attacctggt
     3001 caaaagtgaa aacatcagtt aaaaggtggt ataaagtaaa atatcggtaa taaaaggtgg
     3061 cccaaagtga aatttactct tttctactat tataaaaatt gaggatgttt ttgtcggtac
     3121 tttgatacgt catttttgta tgaattggtt tttaagttta ttcgcttttg gaaatgcata
     3181 tctgtatttg agtcgggttt taagttcgtt tgcttttgta aatacagagg gatttgtata
     3241 agaaatatct ttaaaaaaac ctatatgcta atttgacata atttttgaga aaaatatata
     3301 ttcaggcgaa ttctcacaat gaacaataat aagattaaaa tagctttccc ccgttgcagc
     3361 gcatgggtat tttttctagt aaaaataaaa gataaactta gactcaaaac atttacaaaa
     3421 acaaccccta aagttcctaa agcccaaagt gctatccacg atccatagca agcccagccc
     3481 aacccaaccc aacccaaccc accccagtcc agccaactgg acaatagtct ccacaccccc
     3541 ccactatcac cgtgagttgt ccgcacgcac cgcacgtctc gcagccaaaa aaaaaaaaag
     3601 aaagaaaaaa aagaaaaaga aaaaacagca ggtgggtccg ggtcgtgggg gccggaaacg
     3661 cgaggaggat cgcgagccag cgacgaggcc ggccctccct ccgcttccaa agaaacgccc
     3721 cccatcgcca ctatatacat acccccccct ctcctcccat ccccccaacc ctaccaccac
     3781 caccaccacc acctccacct cctcccccct cgctgccgga cgacgagctc ctcccccctc
     3841 cccctccgcc gccgccgcgc cggtaaccac cccgcccctc tcctctttct ttctccgttt
     3901 tttttttccg tctcggtctc gatctttggc cttggtagtt tgggtgggcg agaggcggct
     3961 tcgtgcgcgc ccagatcggt gcgcgggagg ggcgggatct cgcggctggg gctctcgccg
     4021 gcgtggatcc ggcccggatc tcgcggggaa tggggctctc ggatgtagat ctgcgatccg
     4081 ccgttgttgg gggagatgat ggggggttta aaatttccgc catgctaaac aagatcagga
     4141 agaggggaaa agggcactat ggtttatatt tttatatatt tctgctgctt cgtcaggctt
     4201 agatgtgcta gatctttctt tcttcttttt gtgggtagaa tttgaatccc tcagcattgt
     4261 tcatcggtag tttttctttt catgatttgt gacaaatgca gcctcgtgcg gagctttttt
     4321 gtaggtaggg ctagcctttc gcagatccgg gggggcaatg agatatgaaa aagcctgaac
     4381 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     4441 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     4501 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     4561 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag tttagcgaga
     4621 gcctgaccta ttgcatctcc cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa
     4681 ccgaactgcc cgctgttcta caaccggtcg cggaggctat ggatgcgatc gctgcggccg
     4741 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     4801 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     4861 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     4921 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     4981 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     5041 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     5101 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccacgactc cgggcgtata
     5161 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     5221 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     5281 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     5341 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaagaaatag agtagatgcc
     5401 gaccggatct gtcgaactag cataacttcg tatagcatac attatacgaa gttattcgac
     5461 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     5521 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     5581 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     5641 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     5701 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccca aaacgttaca
     5761 ccacaatata tcctgccaag atctctaatt ccggggatcg gaaatccaga agcccgagag
     5821 gttgccgcct ttcggctttt tctttttcaa aaaaaaaaat ttataaaacg atctgttgcg
     5881 gccggccgcc gggttgtggg caaaggcgct ggcgctcgac ggtgggcaac cgcttgcggt
     5941 tgtccacggg cggagccggt gcgcgtagcg cattgtccac aagccaaggg cgaccaataa
     6001 ttgatatata tattcataat tgaaaagcta attgaacata ctacttgctg taactacttg
     6061 ccggagcgag gggtgtttgc aagctgttga tctgaaaggg ctattagcgt tctcacgtgc
     6121 ctttttgatt agcgatttca cgtgacctta ttagcgattt cacgtactcc gattagcgat
     6181 ttcacgtacc ctgattagcg atttcacgtg gatagttttt ggagcgggcc ggaaagcccc
     6241 gtgaatcaag gctttgcggg gcattagcgg tttcacgtgg ataactaccc tctatccaca
     6301 ggcttccggg gataaaaaag cccgctcgac ggcgggctgt tggatgggga tcgcctgaat
     6361 cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt tgttgtaggt
     6421 ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag
     6481 atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa gccacgttgt
     6541 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     6601 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     6661 gtcttgctca aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     6721 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac cgattgtatg ggaagcccga
     6781 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgttg ttacagatga
     6841 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     6901 ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca
     6961 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     7021 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     7081 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     7141 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     7201 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     7261 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     7321 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     7381 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     7441 tgagtttttc taatcactag accaatgtta cacatatata ctttagattg atttaaaact
     7501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7681 accagcggtg gtttgtttgc cggatcaaga gctaccaact cttcttccga aggtaactgg
     7741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgagatctca
     8281 aacaaacaca tacagcgact tagtttaccc gccaatatat cctgtcaagg ccttcatgtt
     8341 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8401 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8461 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8521 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8581 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     8641 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg
     8701 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag
     8761 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     8821 cgaggggggg ccc
//
