LOCUS       V005911                 6376 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005911
VERSION     V005911
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Yero D, Ferrer-Navarro M, Costenaro L, Planell R, Conchillo-Sole O,
            Sole M, Roca I, Gibert I, Daura X.
  TITLE     A transport system associated with the intrinsic antimicrobial
            multiresistance in Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Yero D, Gibert I.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Genetica Molecular Bacteriana, Institut de
            Biotecnologia i de Biomedicina (IBB), Campus UAB, Bellaterra,
            Barcelona 08191, Spain
REFERENCE   3  (bases 1 to 6376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-DEC-2016) Genetica Molecular Bacteriana, Institut de
            Biotecnologia i de Biomedicina (IBB), Campus UAB, Bellaterra,
            Barcelona 08191, Spain"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            625..734
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             767..1135
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(1101..1526)
                     /codon_start=1
                     /gene="traI"
                     /product="TraI"
                     /label="traI"
                     /protein_id="ATW75324.1"
                     /translation="MNVQVVGVVMHGTDALMLGEAKPSADAVLNRAQRLRVGLLSRAEA
                     NQQVIGLVGLGTGVAVLGRHHLGHDSGQGVCLAVRDAHVTQALGLALLVGDVLHQLREV
                     ALLDGAHGDVLGNHAHPPAVLAAKKVMALPSGGPRPS"
     gene            complement(1101..1526)
                     /gene="traI"
                     /label="traI"
     rep_origin      1756..2144
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     misc_feature    2149..2177
                     /label="multiple cloning site 1"
                     /note="multiple cloning site 1"
     promoter        2193..2221
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             2253..3173
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     misc_feature    3182..3215
                     /label="multiple cloning site 2"
                     /note="multiple cloning site 2"
     CDS             3939..4172
                     /label="TpR"
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     misc_feature    4178..4195
                     /label="I-SceI recognition site"
                     /note="I-SceI recognition site"
     CDS             4582..5316
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
ORIGIN
        1 gatccttttt gtccggtgtt gggttgaagg tgaagccggt cggggccgca gcgggggccg
       61 gcttttcagc cttgcccccc tgcttcggcc gccgtggctc cggcgtcttg ggtgccggcg
      121 cgggttccgc agccttggcc tgcggtgcgg gcacatcggc gggcttggcc ttgatgtgcc
      181 gcctggcgtg cgagcggaac gtctcgtagg agaacttgac cttccccgtt tcccgcatgt
      241 gctcccaaat ggtgacgagc gcatagccgg acgctaacgc cgcctcgaca tccgccctca
      301 ccgccaggaa cgcaaccgca gcctcatcac gccggcgctt cttggccgcg cgggattcaa
      361 cccactcggc cagctcgtcg gtgtagctct ttggcatcgt ctctcgcctg tcccctcagt
      421 tcagtaattt cctgcatttg cctgtttcca gtcggtagat attccacaaa acagcaggga
      481 agcagcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
      541 atccatcctt tttcgcacga tatacaggat taatttgcaa agggttcgtg tagactttcc
      601 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg
      661 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
      721 gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac
      781 caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag
      841 agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt
      901 cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg
      961 catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc
     1021 gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga tcgaagagaa
     1081 gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag agccatgact
     1141 tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc cccatgcgct
     1201 ccatcaagaa gagcgacttc gcggagctgg tgaagtacat caccgacgag caaggcaaga
     1261 ccgagcgcct gggtcacgtg cgcgtcacga actgcgaggc aaacaccctg cccgctgtca
     1321 tggccgaggt gatggcgacc cagcacggca acacccgttc cgaggccgac aagacctatc
     1381 acctgctggt tagcttccgc gcgggagaga agcccgacgc ggagacgttg cgcgcgattg
     1441 aggaccgcat ctgcgctggg cttggcttcg ccgagcatca gcgcgtcagt gccgtgcatc
     1501 acgacaccga caacctgcac attcatatcg ccatcaacaa gattcacccg acccgaaaca
     1561 ccatccatga gccgtatcgg gcctaccgcg ccctcgctga cctctgcgcg acgctcgaac
     1621 gggactacgg gcttgagcgt gacaatcacg aaacgcggca gcgcgtttcc gagaaccgcg
     1681 cgaacgacat ggagcggcac gcgggcgtgg aaagcctggt cggctggatc cggccacgat
     1741 gcgtccggcg tagaggatct gaagatcagc agttcaacct gttgatagta cgtactaagc
     1801 tctcatgttt cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa
     1861 ctaaaccctc atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt
     1921 cacgtactaa gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc
     1981 ctctaaggtt ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa
     2041 ggtttaacgg ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct
     2101 ctcaaagcaa ttttgagtga cacaggaaca cttaacggct gacatgggaa ttcgggcccc
     2161 gctagccaga tcttcccggg aagcttgagc tgttgacaat taatcatcgg ctcgtataat
     2221 gtgtggaata tcacagaagg agacagaata ctatgaacaa aggtgtaatg cgaccgggcc
     2281 atgtgcagct gcgtgtactg gacatgagca aggccctgga acactacgtc gagttgctgg
     2341 gcctgatcga gatggaccgt gacgaccagg gccgtgtcta tctgaaggct tggaccgaag
     2401 tggataagtt ttccctggtg ctacgcgagg ctgacgagcc gggcatggat tttatgggtt
     2461 tcaaggttgt ggatgaggat gctctccggc aactggagcg ggatctgatg gcatatggct
     2521 gtgccgttga gcagctaccc gcaggtgaac tgaacagttg tggccggcgc gtgcgcttcc
     2581 aggccccctc cgggcatcac ttcgagttgt atgcagacaa ggaatatact ggaaagtggg
     2641 gtttgaatga cgtcaatccc gaggcatggc cgcgcgatct gaaaggtatg gcggctgtgc
     2701 gtttcgacca cgccctcatg tatggcgacg aattgccggc gacctatgac ctgttcacca
     2761 aggtgctcgg tttctatctg gccgaacagg tgctggacga aaatggcacg cgcgtcgccc
     2821 agtttctcag tctgtcgacc aaggcccacg acgtggcctt cattcaccat ccggaaaaag
     2881 gccgcctcca tcatgtgtcc ttccacctcg aaacctggga agacttgctt cgcgccgccg
     2941 acctgatctc catgaccgac acatctatcg atatcggccc aacccgccac ggcctcactc
     3001 acggcaagac catctacttc ttcgacccgt ccggtaaccg caacgaagtg ttctgcgggg
     3061 gagattacaa ctacccggac cacaaaccgg tgacctggac caccgaccag ctgggcaagg
     3121 cgatctttta ccacgaccgc attctcaacg aacgattcat gaccgtgctg acctgacctg
     3181 agcggccgcc aatattgcat gcggtaccgt ctagaagaag cttgggatcc gtcggggatc
     3241 cggtgattga ttgagcaagc tttatgcttg taaaccgttt tgtgaaaaaa tttttaaaat
     3301 aaaaaagggg acctctaggg tccccaatta attagtaata taatctatta aaggtcattc
     3361 aaaaggtcat ccaccggatc agcttagtaa agccctcgct agattttaat gcggatgttg
     3421 cgattacttc gccaactatt gcgataacaa gaaaaagcca gcctttcatg atatatctcc
     3481 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     3541 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     3601 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     3661 cctttcacgt agtggacaaa ttcttccaac tgatctagct ttaatgcggt agtttatcac
     3721 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     3781 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     3841 gcctcttgcg ggattctacg gggtctgacg ctcagtggaa cgaaatcgat gagctcgatt
     3901 tgcttgtcgc cataatagat tcacaagaag gattcgacat gggtcaaagt agcgatgaag
     3961 ccaacgctcc cgttgcaggg cagtttgcgc ttcccctgag tgccaccttt ggcttagggg
     4021 atcgcgtacg caagaaatct ggtgccgctt ggcagggtca agtcgtcggt tggtattgca
     4081 caaaactcac tcctgaaggc tatgcggtcg agtccgaatc ccacccaggc tcagtgcaaa
     4141 tttatcctgt ggctgcactt gaacgtgtgg cctaagcatt accctgttat ccctaactgc
     4201 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4261 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4321 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4381 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     4441 gaaacgtaaa agaagttatg gaaataagac ttagaagcaa acttaagagt gtgttgatag
     4501 tgcagtatct taaaattttg tataatagga attgaagtta aattagatgc taaaaatttg
     4561 taattaagaa ggagtgatta catgaacaaa aatataaaat attctcaaaa ctttttaacg
     4621 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
     4681 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
     4741 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
     4801 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
     4861 aggtataaaa ttgttgggag tattccttac catttaagca cacaaattat taaaaaagtg
     4921 gtttttgaaa gccatgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
     4981 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
     5041 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata
     5101 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
     5161 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
     5221 gcaatgaaac acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct
     5281 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttttaa
     5341 atttggaaag ttacacgtta ctaaagggaa tggagataaa ttattagata tactactgac
     5401 agcttccaag aagctaaaga ggtccctagc gcctacgggg aatttgtact gcagtcaatg
     5461 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5521 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5581 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5641 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5701 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5761 cattggtaaa gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5821 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5881 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5941 catatatact ttagattgat ttatggtgca ctctcagtac aatctgctct gatgccgcat
     6001 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     6061 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6121 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6181 acgcgcgagg cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     6241 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     6301 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     6361 tgcgtccggc gtagag
//
