LOCUS       40924_22478        4925 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mutagenesis vector pGPI-SceI-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA.
  TITLE     Aminoarabinose is essential for lipopolysaccharide export and 
            intrinsic antimicrobial peptide resistance in Burkholderia 
            cenocepacia(dagger)
  JOURNAL   Mol. Microbiol. 85 (5), 962-974 (2012)
  PUBMED    22742453
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2012) Microbiology and Immunology, Western 
            University, 1151 Richmond Street North, London, Ontario N6A 5C1, 
            Canada
REFERENCE   3  (bases 1 to 4925)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2012"; volume: "85"; issue: "5"; pages: 
            "962-974"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-DEC-2012) Microbiology and Immunology, Western University, 1151 
            Richmond Street North, London, Ontario N6A 5C1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            626..735
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             768..1136
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             complement(1102..1527)
                     /codon_start=1
                     /product="TraI"
                     /label=TraI
                     /protein_id="AGC79936.1"
                     /translation="MNVQVVGVVMHGTDALMLGEAKPSADAVLNRAQRLRVGLLSRAEA
                     NQQVIGLVGLGTGVAVLGRHHLGHDSGQGVCLAVRDAHVTQALGLALLVGDVLHQLREV
                     ALLDGAHGDVLGNHAHPPAVLAAKKVMALPSGGPRPS"
     rep_origin      1757..2145
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     misc_feature    2149..2175
                     /label=multiple cloning site 1
                     /note="multiple cloning site 1"
     misc_feature    2388..2421
                     /label=multiple cloning site 2
                     /note="multiple cloning site 2"
     promoter        3150..3178
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3504..3737
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    3743..3760
                     /label=I SceI recognition site
                     /note="I SceI recognition site"
ORIGIN
        1 ggatcctttt tgtccggtgt tgggttgaag gtgaagccgg tcggggccgc agcgggggcc
       61 ggcttttcag ccttgccccc ctgcttcggc cgccgtggct ccggcgtctt gggtgccggc
      121 gcgggttccg cagccttggc ctgcggtgcg ggcacatcgg cgggcttggc cttgatgtgc
      181 cgcctggcgt gcgagcggaa cgtctcgtag gagaacttga ccttccccgt ttcccgcatg
      241 tgctcccaaa tggtgacgag cgcatagccg gacgctaacg ccgcctcgac atccgccctc
      301 accgccagga acgcaaccgc agcctcatca cgccggcgct tcttggccgc gcgggattca
      361 acccactcgg ccagctcgtc ggtgtagctc tttggcatcg tctctcgcct gtcccctcag
      421 ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga tattccacaa aacagcaggg
      481 aagcagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
      541 tatccatcct ttttcgcacg atatacagga ttaatttgca aagggttcgt gtagactttc
      601 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
      661 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
      721 tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa
      781 ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa
      841 gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg
      901 tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc
      961 gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc
     1021 cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga
     1081 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
     1141 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
     1201 tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag
     1261 accgagcgcc tgggtcacgt gcgcgtcacg aactgcgagg caaacaccct gcccgctgtc
     1321 atggccgagg tgatggcgac ccagcacggc aacacccgtt ccgaggccga caagacctat
     1381 cacctgctgg ttagcttccg cgcgggagag aagcccgacg cggagacgtt gcgcgcgatt
     1441 gaggaccgca tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag tgccgtgcat
     1501 cacgacaccg acaacctgca cattcatatc gccatcaaca agattcaccc gacccgaaac
     1561 accatccatg agccgtatcg ggcctaccgc gccctcgctg acctctgcgc gacgctcgaa
     1621 cgggactacg ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc cgagaaccgc
     1681 gcgaacgaca tggagcggca cgcgggcgtg gaaagcctgg tcggctggat ccggccacga
     1741 tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     1801 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     1861 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
     1921 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
     1981 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta
     2041 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc
     2101 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatggga attcgggccc
     2161 cgctagccag atcttcccat cggcccaacc cgccacggcc tcactcacgg caagaccatc
     2221 tacttcttcg acccgtccgg taaccgcaac gaagtgttct gcgggggaga ttacaactac
     2281 ccggaccaca aaccggtgac ctggaccacc gaccagctgg gcaaggcgat cttttaccac
     2341 gaccgcattc tcaacgaacg attcatgacc gtgctgacct gacctgagcg gccgccaata
     2401 ttgcatgcgg taccgtctag aagaagcttg ggatccgtcg gggatccggt gattgattga
     2461 gcaagcttta tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct
     2521 ctagggtccc caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac
     2581 cggatcagct tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat tacttcgcca
     2641 actattgcga taacaagaaa aagccagcct ttcatgatat atctcccaat ttgtgtaggg
     2701 cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc
     2761 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
     2821 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     2881 gacaaattct tccaactgat ctagctttaa tgcggtagtt tatcacagtt aaattgctaa
     2941 cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
     3001 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     3061 tctacggggt ctgacgctca gtggaacgaa atcgatgagc tcgcacgaac ccagttgaca
     3121 taagcctgtt cggttcgtaa agaacccagt tgacataagc ctgttcggtt cgtaaactgt
     3181 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3241 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
     3301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggcagc cgttgtgctg
     3421 gtgctttctg atagttgttg tggggtaggc agtcagagct cgatttgctt gtcgccataa
     3481 tagattcaca agaaggattc gacatgggtc aaagtagcga tgaagccaac gctcccgttg
     3541 cagggcagtt tgcgcttccc ctgagtgcca cctttggctt aggggatcgc gtacgcaaga
     3601 aatctggtgc cgcttggcag ggtcaagtcg tcggttggta ttgcacaaaa ctcactcctg
     3661 aaggctatgc ggtcgagtcc gaatcccacc caggctcagt gcaaatttat cctgtggctg
     3721 cacttgaacg tgtggcctaa gcattaccct gttatcccta actgcactca ccagtcacag
     3781 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3841 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3901 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3961 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     4021 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4081 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4141 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4201 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4261 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaaa
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4561 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     4621 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4681 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     4741 cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     4801 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     4861 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     4921 gtaga
//
