LOCUS       40924_22468        6057 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mini-Tn7 delivery vector pGP-Tn7-Gm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in 
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
  PUBMED    22706059
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Microbiology and Biothechnology, 
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval, 
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6057)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6057)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17"; 
            pages: "6001-6008"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2012) Microbiology and Biothechnology, INRS-Institut 
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   189..387
                     /note="Tn7R; right end of Tn7"
     primer_bind     392..408
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      436..530
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      633..719
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    765..812
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(945..1475)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1664..1692)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_recomb     1733..1780
                     /note="FRT; Flp recombinase target site"
     protein_bind    1733..1780
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1807..1892
                     /note="MCS; multiple cloning site"
     mobile_element  complement(1893..2058)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        2454..2558
                     /label=AmpR promoter
     CDS             2559..3416
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(4488..4856)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(4889..4998)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      5642..5998
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aattgtaccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
       61 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctaga
      121 ggaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
      181 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
      241 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
      301 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
      361 tgcccgtcgt attaaagagg ggcgtggggt tcgaggtcga cggtatcgat aagctagctt
      421 aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      481 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagctaga
      541 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
      601 gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag
      661 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      721 cgccgctagg agcttgcggc ccggacgatc gaattgggga tcttgaagta cctattccga
      781 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagctg atgtgcttaa
      841 aaacttactc aatggaattc tagatcgaat tggccgcggc gttgtgacaa tttaccgaac
      901 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
      961 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     1021 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     1081 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     1141 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     1201 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     1261 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     1321 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     1381 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     1441 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     1501 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     1561 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     1621 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     1681 ggcttatgtc aattcgatcc attgctgttg acaaagggaa tcaggggatc ttgaagttcc
     1741 tattccgaag ttcctattct ctagaaagta taggaacttc agagcgcttt tgaagctaat
     1801 tcgatcatgc atgagctcac tagtggatcc cccgggctgc aggaattcct cgagaagctt
     1861 gggcccggta cctcgcgaag gccttgcagg ccaaccagat aagtgaaatc tagttccaaa
     1921 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     1981 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     2041 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttgggc gctgcattaa
     2101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2401 ggactataaa ctcgacctgc agatctgcag gtggcacttt tcggggaaat gtgcgcggaa
     2461 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2521 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2581 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2641 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2701 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2761 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     2821 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2881 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2941 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3001 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3061 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     3121 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3181 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3241 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3301 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3361 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3421 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaatttt
     3481 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     3541 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3601 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3661 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aaggagatgg
     3721 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     3781 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     3841 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatccagc
     3901 cgaccaggct ttccacgccc gcgtgccgct ccatgtcgtt cgcgcggttc tcggaaacgc
     3961 gctgccgcgt ttcgtgattg tcacgctcaa gcccgtagtc ccgttcgagc gtcgcgcaga
     4021 ggtcagcgag ggcgcggtag gcccgatacg gctcatggat ggtgtttcgg gtcgggtgaa
     4081 tcttgttgat ggcgatatgg atgtgcaggt tgtcggtgtc gtgatgcacg gcactgacgc
     4141 gctgatgctc ggcgaagcca agcccagcgc agatgcggtc ctcaatcgcg cgcaacgtct
     4201 ccgcgtcggg cttctctccc gcgcggaagc taaccagcag gtgataggtc ttgtcggcct
     4261 cggaacgggt gttgccgtgc tgggtcgcca tcacctcggc catgacagcg ggcagggtgt
     4321 ttgcctcgca gttcgtgacg cgcacgtgac ccaggcgctc ggtcttgcct tgctcgtcgg
     4381 tgatgtactt caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     4441 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     4501 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     4561 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     4621 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     4681 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     4741 gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     4801 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     4861 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     4921 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     4981 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     5041 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     5101 atgaccccga agcagggtta tgcagcggaa aagcgctgct tccctgctgt tttgtggaat
     5161 atctaccgac tggaaacagg caaatgcagg aaattactga actgagggga caggcgagag
     5221 acgatgccaa agagctacac cgacgagctg gccgagtggg ttgaatcccg cgcggccaag
     5281 aagcgccggc gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga tgtcgaggcg
     5341 gcgttagcgt ccggctatgc gctcgtcacc atttgggagc acatgcggga aacggggaag
     5401 gtcaagttct cctacgagac gttccgctcg cacgccaggc ggcacatcaa ggccaagccc
     5461 gccgatgtgc ccgcaccgca ggccaaggct gcggaacccg cgccggcacc caagacgccg
     5521 gagccacggc ggccgaagca ggggggcaag gctgaaaagc cggcccccgc tgcggccccg
     5581 accggcttca ccttcaaccc aacaccggac aaaaaggatc cagcagttca acctgttgat
     5641 agtacgtact aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc
     5701 tcatgtttaa cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact
     5761 aagctctcat gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca
     5821 gcaacttaaa tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct
     5881 tttaaagctt ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga
     5941 agcccttaga gcctctcaaa gcaattttca gtgacacagg aacacttaac ggctgacatg
     6001 gccttagaag gcgttttaga agccctaatg gatggagtca aaacaaacta gcgatcg
//
