LOCUS       40924_22463        6123 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning and delivery vector pGP-Tn7-Cm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in 
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
  PUBMED    22706059
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Microbiology and Biothechnology, 
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval, 
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6123)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6123)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17"; 
            pages: "6001-6008"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2012) Microbiology and Biothechnology, INRS-Institut 
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   189..387
                     /note="Tn7R; right end of Tn7"
     primer_bind     392..408
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      436..530
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      633..719
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    765..812
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        897..999
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1000..1656
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    1799..1846
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1799..1845
                     /note="FRT; Flp recombinase target site"
     misc_feature    1873..1958
                     /note="MCS; multiple cloning site"
     mobile_element  complement(1959..2124)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        2520..2624
                     /label=AmpR promoter
     CDS             2625..3482
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(4554..4922)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(4955..5064)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      5708..6064
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aattgtaccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
       61 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctaga
      121 ggaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
      181 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
      241 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
      301 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
      361 tgcccgtcgt attaaagagg ggcgtggggt tcgaggtcga cggtatcgat aagctagctt
      421 aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      481 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagctaga
      541 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
      601 gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag
      661 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      721 cgccgctagg agcttgcggc ccggacgatc gaattgggga tcttgaagta cctattccga
      781 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagctg atgtgcttaa
      841 aaacttactc aatggaataa ttctagatgg gccaactttt ggcgaaaatg agacgttgat
      901 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      961 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     1021 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     1081 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     1141 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     1201 aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     1261 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     1321 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     1381 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     1441 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     1501 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     1561 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1621 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     1681 tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga
     1741 aattcgaaag caaattcgac ccatccattg ctgttgacaa agggaatcag gggatcttga
     1801 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcagag cgcttttgaa
     1861 gctaattcga tcatgcatga gctcactagt ggatcccccg ggctgcagga attcctcgag
     1921 aagcttgggc ccggtacctc gcgaaggcct tgcaggccaa ccagataagt gaaatctagt
     1981 tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc
     2041 atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt
     2101 tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt ttgggcgctg
     2161 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2221 tcctcgctca ctgacccgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2281 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2461 ccgacaggac tataaactcg acctgcagat ctgcaggtgg cacttttcgg ggaaatgtgc
     2521 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2581 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2641 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2701 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2761 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2821 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     2881 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2941 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3001 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3061 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3121 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     3181 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3241 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3301 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3361 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3421 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3481 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3541 aattttggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3601 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3661 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3721 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcaagg
     3781 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     3841 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     3901 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     3961 tccagccgac caggctttcc acgcccgcgt gccgctccat gtcgttcgcg cggttctcgg
     4021 aaacgcgctg ccgcgtttcg tgattgtcac gctcaagccc gtagtcccgt tcgagcgtcg
     4081 cgcagaggtc agcgagggcg cggtaggccc gatacggctc atggatggtg tttcgggtcg
     4141 ggtgaatctt gttgatggcg atatggatgt gcaggttgtc ggtgtcgtga tgcacggcac
     4201 tgacgcgctg atgctcggcg aagccaagcc cagcgcagat gcggtcctca atcgcgcgca
     4261 acgtctccgc gtcgggcttc tctcccgcgc ggaagctaac cagcaggtga taggtcttgt
     4321 cggcctcgga acgggtgttg ccgtgctggg tcgccatcac ctcggccatg acagcgggca
     4381 gggtgtttgc ctcgcagttc gtgacgcgca cgtgacccag gcgctcggtc ttgccttgct
     4441 cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     4501 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     4561 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     4621 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     4681 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     4741 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     4801 taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     4861 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     4921 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
     4981 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     5041 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     5101 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     5161 gctataatga ccccgaagca gggttatgca gcggaaaagc gctgcttccc tgctgttttg
     5221 tggaatatct accgactgga aacaggcaaa tgcaggaaat tactgaactg aggggacagg
     5281 cgagagacga tgccaaagag ctacaccgac gagctggccg agtgggttga atcccgcgcg
     5341 gccaagaagc gccggcgtga tgaggctgcg gttgcgttcc tggcggtgag ggcggatgtc
     5401 gaggcggcgt tagcgtccgg ctatgcgctc gtcaccattt gggagcacat gcgggaaacg
     5461 gggaaggtca agttctccta cgagacgttc cgctcgcacg ccaggcggca catcaaggcc
     5521 aagcccgccg atgtgcccgc accgcaggcc aaggctgcgg aacccgcgcc ggcacccaag
     5581 acgccggagc cacggcggcc gaagcagggg ggcaaggctg aaaagccggc ccccgctgcg
     5641 gccccgaccg gcttcacctt caacccaaca ccggacaaaa aggatccagc agttcaacct
     5701 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact
     5761 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa
     5821 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat
     5881 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat
     5941 aaggctttta aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca
     6001 ctgagaagcc cttagagcct ctcaaagcaa ttttcagtga cacaggaaca cttaacggct
     6061 gacatggcct tagaaggcgt tttagaagcc ctaatggatg gagtcaaaac aaactagcga
     6121 tcg
//
