LOCUS       62056_11850        3092 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        311..415
                     /label=AmpR promoter
     CDS             416..1273
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1422..2234
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2322..2910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcggc cagcccctca gcgctgaggg gctggcaagt gtagcggtca cgctgcgcgt
      241 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
      301 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      361 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      421 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      481 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      541 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      601 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      661 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      721 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      781 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      841 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      901 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      961 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1021 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1081 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1141 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1201 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1261 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1321 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaacaa
     1381 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1441 aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     1501 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
     1561 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     1621 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     1681 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat
     1741 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     1801 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     1861 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     1921 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     1981 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2041 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2101 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2161 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     2221 tcgatgagtt tttctaagaa ttaattcatg accaaaatcc cttaacgtga gttttcgttc
     2281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2461 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3061 cgcagcgagt cagtgagcga ggaagcggaa ga
//
