LOCUS       62056_11845        7166 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2459..3175
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4007..4128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4135..4590)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4617..4721
                     /label=AmpR promoter
     promoter        4723..5080
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5115..5906
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6141..6188
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6517..7105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaagaactt ctcacatgaa tgtgattgtg ttactaacgt tattatttgt gatatctttc
       61 tgttaaattg cttaaaaaat tcattactca tttattttct tttcggctta gtccctttat
      121 taatccgggg ttgcaacagc ggaatgaacc gctaacctat ccagcatgtt tctacgcagc
      181 ggatgccctt cagccgcaac ccatctctgg gaaacatcca cacacactca tttacactca
      241 tacactacag aaaatttagc ctacccaatt aacctgtatg tctttggact tgttagggaa
      301 accggagcat ctggaggaaa tcagggagaa catgcaaact ccacacagaa acgtcaactg
      361 atccagccga ggctggaatc agagaccttc ttgctatgag gcgacagcac tacctcctgc
      421 gccacttcgt caccctttct taaaatattg aattatgata aaatcgatta catttaatat
      481 tatatttaaa atgttgtgag acttgaacaa aatcttaatt cacattttaa agagatttat
      541 tcacccttga cttgttattt tgtttgcatt tatattttgg ttttgttgaa tgccattttt
      601 tagaatggtg gaaaccagta atcattgact tccgtagtat tagattttcg tatttttcat
      661 gggtgtcaat ggttatcggt ttccaacatt tctcaaaaca tcttcttttg tatttaaagt
      721 aacaaaaaaa aaggtttcga acaagccaag ggagaattaa tgacgacaga atttccattt
      781 tggggtgaac tatcctatta atattttaat tagctaagtt acattcaaaa gtctaatcaa
      841 aggtggcaat aaaagatgtt taatgttaaa attcaagcag cacaactgtt ttcaaaatgt
      901 cttctgacac ctgtaaagac atataaagta aagggtttat gaggaaatag aattaaaaag
      961 taccatcaaa tgctaaaatg gctcttatat gattcagtct gtgacacaaa cattacctct
     1021 tggctcatct gttcccctga gtgttgatct ggccatcaga gagtcggttt gaaccattca
     1081 aagattattt catgtattgc tgggtttaac catgtccatt agcaaggtgc ccttgtcaaa
     1141 tctttgtttc agtttttgta aatgcagccc cgagtgtaca ttaaaacctg cattagcgac
     1201 aagtctgcac ctgctctgtg gttgcaggag tcttgatgac gaaagtgcag tacatctcat
     1261 tttaaaagga ctatatgaag gagaccaaga acagcagaac tcatcttgaa caaacacagc
     1321 aggtaagaga ttctttactt tcaaaattct gtattgtttt ctcaatctag aatttagaca
     1381 gagtgtgata gaaatgttca tattaataat aaaatactgt acaaacaaaa gaaatacaaa
     1441 aatagcctat atcttttgtg aaattacaat tttgccacat aaattgctgt gggttttgtc
     1501 agcaagttca ctgcaaaaaa aaacctttct ttcttggttt tttttttcta gcccaaattt
     1561 cctaaaattc ttaaatcaag aaaaattttc tagacaagca aagcatattg tcttgtttta
     1621 agaaaaatat gccaaaaaaa gtttttcctt aaaacaagca aagtaatctg ccaatagggt
     1681 aggaaaaata atcctatgtc aaaaagaaaa acaagattat tttgctctcc tccattgcag
     1741 atcattttgc ttgttttaag gaaaaactta cttaatatta gcatattatt tctgtaaata
     1801 agacattatg ttttgcttgc ctaaatattt cttgatataa gaattttttg atatttggac
     1861 tagaaacaag ataaaaaaaa ttaagtaaga gcaggagttt ttgcagtgtt gtacttttat
     1921 atttagtggt ttcaattaag gatggtgcaa tattaatatg tttgagtact tattaactct
     1981 tattatatta ttaataaccc tataggacaa tgcttgttga aattcccatt ggaaaacccc
     2041 ctggtattgt tactgggggt tattatgaca cttttactct gttatgactt cattttactc
     2101 taatagattt atatcaatta attgcaaata gtaaggaata aaaaaaagct aaaataaaat
     2161 acaaagcaaa acaatacaat gcaatttaaa aaatgatttt tttattcttt cctattattg
     2221 ctactttttt catatatttt gagatggaag acaatccttt atttttctca gttgtaaata
     2281 aagatacttg aaaatacttc tttcagttag tctactattc atttcacata ttatagcgaa
     2341 actgcacatt atatttagga ttttttgtgc tgtttgtttg tgtttgtgtg attttttttt
     2401 acagatacaa ataaacaagt gatttctgtg ttttagtttt agcatggagc tagccaccat
     2461 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2521 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2581 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2641 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     2701 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2761 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2821 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2881 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2941 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     3001 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     3061 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     3121 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
     3181 actcagatct cgaggcggcg gagggagtgg aggaggggga agcatggata tcatttctgt
     3241 cgccttaaag cgtcattcca ctaaggcatt tgatgccagc aaaaaactta ccccggaaca
     3301 ggccgagcag atcaaaacgc tactgcaata cagcccatcc agcaccaact cccagccgtg
     3361 gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat ccgctgccgg
     3421 taattacgtg ttcaacgagc gtaaaatgct tgatgcctcg cacgtcgtgg tgttctgtgc
     3481 aaaaaccgcg atggacgatg tctggctgaa gctggttgtt gaccaggaag atgccgatgg
     3541 ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt tcttcgctga
     3601 tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg tttatctcaa
     3661 cgtcggtaac ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac ccatcgaagg
     3721 ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa gagaaaggct acaccagtct
     3781 ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt aacgctacgc tgccgaaatc
     3841 tcgtctgccg caaaacatca ccttaaccga agtgtaagga tccaccggat ctagataact
     3901 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3961 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     4021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     4141 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     4201 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     4261 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     4321 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4381 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4441 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4501 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4561 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     4621 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4681 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     4741 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4801 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4981 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5041 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     5101 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5161 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     5221 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5281 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5341 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5401 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5461 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5521 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5581 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5641 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5701 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5761 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5821 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5881 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5941 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6001 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6061 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     6121 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     6181 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     6241 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     6301 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     6361 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6421 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6481 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6601 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6661 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6721 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6781 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6841 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6901 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6961 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7021 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7081 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7141 cttttgctgg ccttttgctc acatgt
//
