LOCUS       40924_22443        9291 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGMCSFlacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9291)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1082
                     /label=5' flanking region of mCsf2
                     /note="5' flanking region of mCsf2"
     regulatory      1083..2222
                     /label=mCsf2 promoter
                     /note="mCsf2 promoter"
                     /regulatory_class="promoter"
     CDS             2647..5691
                     /label=lacZ
                     /note="beta-galactosidase"
     polyA_signal    5958..6092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6126..6144)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6165..6181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6189..6205)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6213..6243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6258..6279)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6567..7155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7329..8186)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8187..8291)
                     /label=AmpR promoter
     rep_origin      complement(8625..9080)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9221..9237
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9244..9262
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttctgcctcc ctgaagaatg ttgatcatca ggatgggtca gaccatctta aatgctcatg
       61 tgtctgtccc gtgggatgag agggactttg gggcccattt ccttccctcc tggtgttgac
      121 ttcctcttgt gataaagatc ctggggcagc cacacgcttc tggttccccc agggcccatg
      181 tcaagggaca ctccccggaa actccttcca gagggttctc tcctggcctt gtgggctggg
      241 aggccaaaga ggggtcactc cacccatctg gtccccacct ccgccctgcc acagccccac
      301 tgaggccctt gagtcagcaa ggctgtctga tgctattgcc gcttctttcc atgcgtgcaa
      361 tctaggaatg cctgcccgtg tcaccgcctc caccctgatg tcatctttct aggagaaaga
      421 tacatatcta cccacaaact gcatccccaa gcccttagaa tcctgcccca taagtgacaa
      481 ctttggcacc tgtagaccat atccttgacg tgagtcttga ctctggggcc tctgctttcc
      541 tcttagccac caccctgcca cacgcttggg ctaagacgaa tgcctttagt tcttcaacat
      601 ggccattctg gccccccagt ctctctttct acatgattcc tgtggtcttg tcctgagttc
      661 cacctgttat ctgaccctcg aaagccctca cttctgtctg gtttcatcag tccactgaat
      721 cagcttcttg ggccctaaag catgtttctt ggctaggggc cccctctcat atcaagaggg
      781 caccgccaga agcccaaggg ttctcacaaa gcaggcaaaa caagtgtggc tcatgttaga
      841 tgcaccctta tgtaaaacct cctccacctt gacatgttcc tatgagtaca tatatatcca
      901 acaccaccag catacacaca tgactgaaga gatacacata tgtatcccca tggtctcaaa
      961 cttcctgtat aggaaggctt gggccagatc ccttgggcaa acacaaactc gtgccgtctt
     1021 ctccaaaagc cattgtgaaa atcaaacact tgttagcccc ttgagaccat gctgcctttc
     1081 cagaagcagt tcctgattcc acagagagct tctggagagg gaggtgggct ggagcaaggg
     1141 gctgtgcaac agactaagcc tgggagaact tgccagggaa gcgggggttg gggcgcacgt
     1201 gtgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcgcgtgcgt gtgtgcgtgc ctggttattg
     1261 tgttacagct agttactatg ttacagccag cctcagagac ccaggtatcc cataatggta
     1321 cagatagcag taatgtgtcc tttttgaatg gcaggctgct ctgaagaggg taaagaggct
     1381 cacataactc aaaggaagag tcgcttggca aagggggagg gcagaatcag cagtcaaatg
     1441 ggagtagcct tacctgcccc agccagaagc tctagccatg ctgtgaattt ttaggattga
     1501 acatgccttt gtaactggga ttgtagcagt cagttgggat ggtatcataa gagaacatgg
     1561 tatagtctgc ctggcttcta tacccattgc tgtcattctc actgctccca aggtggcagg
     1621 ggtggggctt ggctgggcag tttgcccaga atggtctcct cagtgggagt ctgtggagac
     1681 cattatagaa ttgtgtcatt gggctggacc ttatttaata gataatgagg tggacttgtg
     1741 agaagtgtat atctcagaag gtggctggaa agagaacggg ccaggactgg ggctggaatg
     1801 agccaccaga gtaggtagag cttgcccaaa ggcctccagg aacagcaggt gctatggaag
     1861 caagagcccc actcagtatc tcccaaaccc cgccccagcc actccaggcc aggaaatcca
     1921 aatatgcctg gaggcccctc aaaaaggaga ggctagccag aggctgggtc agactgccca
     1981 ggcagggtgg gaaaggcctt taatcagccc gcaggtgggc tgccagttct tggaagggct
     2041 tattaatgaa aaccccccaa gcctgacaac ctgggggaag gctcactggc cccatgtata
     2101 gctgataagg gccaggagat tccacaactc aggtagttcc cccgcccccc tggagttctg
     2161 tggtcaccat taatcatttc ctctaactgt gtatataaga gctcttttgc agtgagccca
     2221 gtgacctgca ggcatgcaag cttggacaca agacaggctt gcgagatatg tttgagaata
     2281 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     2341 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     2401 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     2461 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     2521 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cggtgggtga agaccagaaa
     2581 cagcacctcg aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc
     2641 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2701 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2761 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
     2821 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
     2881 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc
     2941 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
     3001 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
     3061 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
     3121 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
     3181 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
     3241 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
     3301 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg
     3361 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
     3421 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
     3481 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
     3541 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
     3601 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
     3661 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
     3721 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc
     3781 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc
     3841 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg
     3901 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc
     3961 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct
     4021 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
     4081 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
     4141 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
     4201 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
     4261 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
     4321 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
     4381 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
     4441 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt
     4501 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat
     4561 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt
     4621 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg
     4681 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc
     4741 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc
     4801 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat
     4861 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca
     4921 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt
     4981 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg
     5041 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg
     5101 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg
     5161 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt
     5221 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc
     5281 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat
     5341 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat
     5401 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat
     5461 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa
     5521 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc
     5581 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa
     5641 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     5701 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa
     5761 aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc
     5821 tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg
     5881 ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg
     5941 ctttctgaca aactcggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6061 aactcatcaa tgtatcttat catgtctgga tccccgaggg ggggcccggt acccagcttt
     6121 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     6181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6361 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6421 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6481 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6841 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6961 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7021 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7081 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7141 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7201 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7261 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7321 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7381 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7441 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7501 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7561 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7621 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7681 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7741 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7801 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7861 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7921 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7981 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8041 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8101 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8161 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8221 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8281 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8341 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8401 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8461 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8521 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     8581 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgaaattg taaacgttaa
     8641 tattttgtta aaattcgcgt taaatatttg ttaaatcagc tcatttttta accaataggc
     8701 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8761 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8821 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     8881 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8941 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     9001 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     9061 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9121 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9181 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     9241 ttgtaatacg actcactata gggcgaattg gccaagtcgg ccgagctcga a
//
