LOCUS       62056_11840        9375 bp DNA     circular SYN 15-FEB-2020
DEFINITION  Expression vector pGM74, complete sequence.
ACCESSION   MN811114
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for 
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
  PUBMED    32001704
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Krawczyk KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        40..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        420..623
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             730..750
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             754..774
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             805..4905
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     polyA_signal    4975..5199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5245..5673
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5687..6016
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6083..6874
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7051..7184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7221..7237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7245..7261)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7269..7299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7314..7335)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7623..8208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8382..9239)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9240..9344)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctcca cgcgtggtac cctcgagttg acattgatta ttgagtagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      481 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      541 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      601 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      661 tcgaaattaa tacgactcac tatagggaga cccaagctgt tcgaagcgga attcaccatg
      721 actagtgatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtagatcca
      781 aaaaagaaga gaaaggtagc tagtgacaag aagtattcta tcggactggc catcgggact
      841 aatagcgtcg ggtgggccgt gatcactgac gagtacaagg tgccctctaa gaagttcaag
      901 gtgctcggga acaccgaccg gcattccatc aagaaaaatc tgatcggagc tctcctcttt
      961 gattcagggg agaccgctga agcaacccgc ctcaagcgga ctgctagacg gcggtacacc
     1021 aggaggaaga accggatttg ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc
     1081 gacgacagct tcttccatag gctggaagaa tcattcctcg tggaagagga taagaagcat
     1141 gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg cctatcacga gaaataccca
     1201 accatctacc atcttcgcaa aaagctggtg gactcaaccg acaaggcaga cctccggctt
     1261 atctacctgg ccctggccca catgatcaag ttcagaggcc acttcctgat cgagggcgac
     1321 ctcaatcctg acaatagcga tgtggataaa ctgttcatcc agctggtgca gacttacaac
     1381 cagctctttg aagagaaccc catcaatgca agcggagtcg atgccaaggc cattctgtca
     1441 gcccggctgt caaagagccg cagacttgag aatcttatcg ctcagctgcc gggtgaaaag
     1501 aaaaatggac tgttcgggaa cctgattgct ctttcacttg ggctgactcc caatttcaag
     1561 tctaatttcg acctggcaga ggatgccaag ctgcaactgt ccaaggacac ctatgatgac
     1621 gatctcgaca acctcctggc ccagatcggt gaccaatacg ccgacctttt ccttgctgct
     1681 aagaatcttt ctgacgccat cctgctgtct gacattctcc gcgtgaacac tgaaatcacc
     1741 aaggcccctc tttcagcttc aatgattaag cggtatgatg agcaccacca ggacctgacc
     1801 ctgcttaagg cactcgtccg gcagcagctt ccggagaagt acaaggaaat cttctttgac
     1861 cagtcaaaga atggatacgc cggctacatc gacggaggtg cctcccaaga ggaattttat
     1921 aagtttatca aacctatcct tgagaagatg gacggcaccg aagagctcct cgtgaaactg
     1981 aatcgggagg atctgctgcg gaagcagcgc actttcgaca atgggagcat tccccaccag
     2041 atccatcttg gggagcttca cgccatcctt cggcgccaag aggacttcta cccctttctt
     2101 aaggacaaca gggagaagat tgagaaaatt ctcactttcc gcatccccta ctacgtggga
     2161 cccctcgcca gaggaaatag ccggtttgct tggatgacca gaaagtcaga agaaactatc
     2221 actccctgga acttcgaaga ggtggtggac aagggagcca gcgctcagtc attcatcgaa
     2281 cggatgacta acttcgataa gaacctcccc aatgagaagg tcctgccgaa acattccctg
     2341 ctctacgagt actttaccgt gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg
     2401 atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg caattgtgga ccttctgttc
     2461 aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa
     2521 tgcttcgact ctgtggagat tagcggggtc gaagatcggt tcaacgcaag cctgggtacc
     2581 taccatgatc tgcttaagat catcaaggac aaggattttc tggacaatga ggagaacgag
     2641 gacatccttg aggacattgt cctgactctc actctgttcg aggaccggga aatgatcgag
     2701 gagaggctta agacctacgc ccatctgttc gacgataaag tgatgaagca acttaaacgg
     2761 agaagatata ccggatgggg acgccttagc cgcaaactca tcaacggaat ccgggacaaa
     2821 cagagcggaa agaccattct tgatttcctt aagagcgacg gattcgctaa tcgcaacttc
     2881 atgcaactta tccatgatga ttccctgacc tttaaggagg acatccagaa ggcccaagtg
     2941 tctggacaag gtgactcact gcacgagcat atcgcaaatc tggctggttc acccgctatt
     3001 aagaagggta ttctccagac cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc
     3061 cataaaccag agaacattgt catcgagatg gccagggaaa accagactac ccagaaggga
     3121 cagaagaaca gcagggagcg gatgaaaaga attgaggaag ggattaagga gctcgggtca
     3181 cagatcctta aagagcaccc ggtggaaaac acccagcttc agaatgagaa gctctatctg
     3241 tactaccttc aaaatggacg cgatatgtat gtggaccaag agcttgatat caacaggctc
     3301 tcagactacg acgtggacgc catcgtccct cagagcttcc tcaaagacga ctcaattgac
     3361 aataaggtgc tgactcgctc agacaagaac cggggaaagt cagataacgt gccctcagag
     3421 gaagtcgtga aaaagatgaa gaactattgg cgccagcttc tgaacgcaaa gctgatcact
     3481 cagcggaagt tcgacaatct cactaaggct gagaggggcg gactgagcga actggacaaa
     3541 gcaggattca ttaaacggca acttgtggag actcggcaga ttactaaaca tgtcgcccaa
     3601 atccttgact cacgcatgaa taccaagtac gacgaaaacg acaaacttat ccgcgaggtg
     3661 aaggtgatta ccctgaagtc caagctggtc agcgatttca gaaaggactt tcaattctac
     3721 aaagtgcggg agatcaataa ctatcatcat gctcatgacg catatctgaa tgccgtggtg
     3781 ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg agttcgtgta cggagactac
     3841 aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc aggagatcgg aaaggccacc
     3901 gcaaagtact tcttctacag caacatcatg aatttcttca agaccgaaat cacccttgca
     3961 aacggtgaga tccggaagag gccgctcatc gagactaatg gggagactgg cgaaatcgtg
     4021 tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac
     4081 atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta aggaatcaat cctccccaag
     4141 cgcaactccg acaagctcat tgcaaggaag aaggattggg accctaagaa gtacggcgga
     4201 ttcgattcac caactgtggc ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag
     4261 tctaagaagc tcaagagcgt gaaggaactg ctgggtatca ccattatgga gcgcagctcc
     4321 ttcgagaaga acccaattga ctttctcgaa gccaaaggtt acaaggaagt caagaaggac
     4381 cttatcatca agctcccaaa gtatagcctg ttcgaactgg agaatgggcg gaagcggatg
     4441 ctcgcctccg ctggcgaact tcagaagggt aatgagctgg ctctcccctc caagtacgtg
     4501 aatttcctct accttgcaag ccattacgag aagctgaagg ggagccccga ggacaacgag
     4561 caaaagcaac tgtttgtgga gcagcataag cattatctgg acgagatcat tgagcagatt
     4621 tccgagtttt ctaaacgcgt cattctcgct gatgccaacc tcgataaagt ccttagcgca
     4681 tacaataagc acagagacaa accaattcgg gagcaggctg agaatatcat ccacctgttc
     4741 accctcacca atcttggtgc ccctgccgca ttcaagtact tcgacaccac catcgaccgg
     4801 aaacgctata cctccaccaa agaagtgctg gacgccaccc tcatccacca gagcatcacc
     4861 ggactttacg aaactcggat tgacctctca cagctcggag gggatgaggg agctgctagc
     4921 ggatccaccg gtgtctagaa agctttgagg ccggcctgca gggatcagcc tcgactgtgc
     4981 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5041 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5101 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5161 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5221 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5281 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5341 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5401 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5461 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5521 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5581 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5641 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5701 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5761 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5821 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5881 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5941 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6001 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     6061 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     6121 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6181 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6241 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6301 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6361 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6421 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6481 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6541 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6601 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6661 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6721 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6781 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6841 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6901 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     7021 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     7081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     7201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8041 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9361 gtgccacctg acgtc
//
