LOCUS       62056_11835        9297 bp DNA     circular SYN 15-FEB-2020
DEFINITION  Expression vector pGM47, complete sequence.
ACCESSION   MN811115
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for 
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
  PUBMED    32001704
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Kim H, Melchner G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 9297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        40..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        420..623
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             727..4827
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     polyA_signal    4897..5121
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5167..5595
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5609..5938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6005..6796
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6973..7106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7143..7159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7167..7183)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7191..7221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7236..7257)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7545..8130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8304..9161)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9162..9266)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctcca cgcgtggtac cctcgagttg acattgatta ttgagtagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      481 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      541 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      601 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      661 tcgaaattaa tacgactcac tatagggaga cccaagctgt tcgaagcgga attcaccatg
      721 actagtgaca agaagtattc tatcggactg gccatcggga ctaatagcgt cgggtgggcc
      781 gtgatcactg acgagtacaa ggtgccctct aagaagttca aggtgctcgg gaacaccgac
      841 cggcattcca tcaagaaaaa tctgatcgga gctctcctct ttgattcagg ggagaccgct
      901 gaagcaaccc gcctcaagcg gactgctaga cggcggtaca ccaggaggaa gaaccggatt
      961 tgttaccttc aagagatatt ctccaacgaa atggcaaagg tcgacgacag cttcttccat
     1021 aggctggaag aatcattcct cgtggaagag gataagaagc atgaacggca tcccatcttc
     1081 ggtaatatcg tcgacgaggt ggcctatcac gagaaatacc caaccatcta ccatcttcgc
     1141 aaaaagctgg tggactcaac cgacaaggca gacctccggc ttatctacct ggccctggcc
     1201 cacatgatca agttcagagg ccacttcctg atcgagggcg acctcaatcc tgacaatagc
     1261 gatgtggata aactgttcat ccagctggtg cagacttaca accagctctt tgaagagaac
     1321 cccatcaatg caagcggagt cgatgccaag gccattctgt cagcccggct gtcaaagagc
     1381 cgcagacttg agaatcttat cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg
     1441 aacctgattg ctctttcact tgggctgact cccaatttca agtctaattt cgacctggca
     1501 gaggatgcca agctgcaact gtccaaggac acctatgatg acgatctcga caacctcctg
     1561 gcccagatcg gtgaccaata cgccgacctt ttccttgctg ctaagaatct ttctgacgcc
     1621 atcctgctgt ctgacattct ccgcgtgaac actgaaatca ccaaggcccc tctttcagct
     1681 tcaatgatta agcggtatga tgagcaccac caggacctga ccctgcttaa ggcactcgtc
     1741 cggcagcagc ttccggagaa gtacaaggaa atcttctttg accagtcaaa gaatggatac
     1801 gccggctaca tcgacggagg tgcctcccaa gaggaatttt ataagtttat caaacctatc
     1861 cttgagaaga tggacggcac cgaagagctc ctcgtgaaac tgaatcggga ggatctgctg
     1921 cggaagcagc gcactttcga caatgggagc attccccacc agatccatct tggggagctt
     1981 cacgccatcc ttcggcgcca agaggacttc tacccctttc ttaaggacaa cagggagaag
     2041 attgagaaaa ttctcacttt ccgcatcccc tactacgtgg gacccctcgc cagaggaaat
     2101 agccggtttg cttggatgac cagaaagtca gaagaaacta tcactccctg gaacttcgaa
     2161 gaggtggtgg acaagggagc cagcgctcag tcattcatcg aacggatgac taacttcgat
     2221 aagaacctcc ccaatgagaa ggtcctgccg aaacattccc tgctctacga gtactttacc
     2281 gtgtacaacg agctgaccaa ggtgaaatat gtcaccgaag ggatgaggaa gcccgcattc
     2341 ctgtcaggcg aacaaaagaa ggcaattgtg gaccttctgt tcaagaccaa tagaaaggtg
     2401 accgtgaagc agctgaagga ggactatttc aagaaaattg aatgcttcga ctctgtggag
     2461 attagcgggg tcgaagatcg gttcaacgca agcctgggta cctaccatga tctgcttaag
     2521 atcatcaagg acaaggattt tctggacaat gaggagaacg aggacatcct tgaggacatt
     2581 gtcctgactc tcactctgtt cgaggaccgg gaaatgatcg aggagaggct taagacctac
     2641 gcccatctgt tcgacgataa agtgatgaag caacttaaac ggagaagata taccggatgg
     2701 ggacgcctta gccgcaaact catcaacgga atccgggaca aacagagcgg aaagaccatt
     2761 cttgatttcc ttaagagcga cggattcgct aatcgcaact tcatgcaact tatccatgat
     2821 gattccctga cctttaagga ggacatccag aaggcccaag tgtctggaca aggtgactca
     2881 ctgcacgagc atatcgcaaa tctggctggt tcacccgcta ttaagaaggg tattctccag
     2941 accgtgaaag tcgtggacga gctggtcaag gtgatgggtc gccataaacc agagaacatt
     3001 gtcatcgaga tggccaggga aaaccagact acccagaagg gacagaagaa cagcagggag
     3061 cggatgaaaa gaattgagga agggattaag gagctcgggt cacagatcct taaagagcac
     3121 ccggtggaaa acacccagct tcagaatgag aagctctatc tgtactacct tcaaaatgga
     3181 cgcgatatgt atgtggacca agagcttgat atcaacaggc tctcagacta cgacgtggac
     3241 gccatcgtcc ctcagagctt cctcaaagac gactcaattg acaataaggt gctgactcgc
     3301 tcagacaaga accggggaaa gtcagataac gtgccctcag aggaagtcgt gaaaaagatg
     3361 aagaactatt ggcgccagct tctgaacgca aagctgatca ctcagcggaa gttcgacaat
     3421 ctcactaagg ctgagagggg cggactgagc gaactggaca aagcaggatt cattaaacgg
     3481 caacttgtgg agactcggca gattactaaa catgtcgccc aaatccttga ctcacgcatg
     3541 aataccaagt acgacgaaaa cgacaaactt atccgcgagg tgaaggtgat taccctgaag
     3601 tccaagctgg tcagcgattt cagaaaggac tttcaattct acaaagtgcg ggagatcaat
     3661 aactatcatc atgctcatga cgcatatctg aatgccgtgg tgggaaccgc cctgatcaag
     3721 aagtacccaa agctggaaag cgagttcgtg tacggagact acaaggtcta cgacgtgcgc
     3781 aagatgattg ccaaatctga gcaggagatc ggaaaggcca ccgcaaagta cttcttctac
     3841 agcaacatca tgaatttctt caagaccgaa atcacccttg caaacggtga gatccggaag
     3901 aggccgctca tcgagactaa tggggagact ggcgaaatcg tgtgggacaa gggcagagat
     3961 ttcgctaccg tgcgcaaagt gctttctatg cctcaagtga acatcgtgaa gaaaaccgag
     4021 gtgcaaaccg gaggcttttc taaggaatca atcctcccca agcgcaactc cgacaagctc
     4081 attgcaagga agaaggattg ggaccctaag aagtacggcg gattcgattc accaactgtg
     4141 gcttattctg tcctggtcgt ggctaaggtg gaaaaaggaa agtctaagaa gctcaagagc
     4201 gtgaaggaac tgctgggtat caccattatg gagcgcagct ccttcgagaa gaacccaatt
     4261 gactttctcg aagccaaagg ttacaaggaa gtcaagaagg accttatcat caagctccca
     4321 aagtatagcc tgttcgaact ggagaatggg cggaagcgga tgctcgcctc cgctggcgaa
     4381 cttcagaagg gtaatgagct ggctctcccc tccaagtacg tgaatttcct ctaccttgca
     4441 agccattacg agaagctgaa ggggagcccc gaggacaacg agcaaaagca actgtttgtg
     4501 gagcagcata agcattatct ggacgagatc attgagcaga tttccgagtt ttctaaacgc
     4561 gtcattctcg ctgatgccaa cctcgataaa gtccttagcg catacaataa gcacagagac
     4621 aaaccaattc gggagcaggc tgagaatatc atccacctgt tcaccctcac caatcttggt
     4681 gcccctgccg cattcaagta cttcgacacc accatcgacc ggaaacgcta tacctccacc
     4741 aaagaagtgc tggacgccac cctcatccac cagagcatca ccggacttta cgaaactcgg
     4801 attgacctct cacagctcgg aggggatgag ggagctgcta gcggatccac cggtgtctag
     4861 aaagctttga ggccggcctg cagggatcag cctcgactgt gccttctagt tgccagccat
     4921 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4981 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5041 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5101 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5161 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5221 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5281 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5341 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5401 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5461 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5521 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5581 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5761 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5821 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5881 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5941 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     6001 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6061 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6121 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6181 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6241 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     6301 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     6361 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     6421 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     6481 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     6541 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     6601 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     6661 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6721 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6781 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6841 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6901 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6961 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7081 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     7141 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7201 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7261 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7321 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7381 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7441 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7501 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7741 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7981 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag
     8101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8221 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8281 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8341 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8401 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8461 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8521 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8581 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8641 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8701 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8761 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8821 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8881 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8941 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9001 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9061 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9241 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
