LOCUS       V013330                 7089 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013330
VERSION     V013330
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(467..576)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             892..1692
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     rep_origin      1994..2582
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2870..2891
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2906..2936
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2944..2960
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2968..2984
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(3163..3197)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             3376..3393
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3403..3420
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             3424..3456
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             3506..3523
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      complement(3688..3736)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             4079..4885
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             5451..6890
                     /label="pSG5 Rep"
                     /note="replication initiator protein from the Streptomyces
                     ghanaensis plasmid pSG5"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
      181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
      241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
      301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
      361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
      421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
      481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
      541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
      601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
      661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
      721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
      781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
      841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
      901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
      961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
     1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
     1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
     1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
     3001 acgtaggcgt cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg
     3061 actggattcc aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg
     3121 acatggctcg attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg
     3181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg
     3241 ggatctgggc tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc
     3301 cgggcttgca cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg
     3361 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
     3421 catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccgaatt cgagctccgt
     3481 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
     3541 cccagatcta aagttttgtc gtctttccag acgttagtaa atgaattttc tgtatgaggt
     3601 tttgctaaac aactttcaac agtttcagcg gagtgagaat agaaaggaac aactaaagga
     3661 attgcgaata ataatttttt cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc
     3721 ctttaattgt atcggtttat cagcttgctt tcgaggtgaa tttcttaaac agcttgatac
     3781 cgatagttgc gccgacaatg acaacaacca tcgcccacgc ataaccgata tattcggtcg
     3841 ctgaggcttg cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg cactagaggc
     3901 ctatatgcgg ggatcgaccg cgcgggtccc ggacggggaa gagcggggag ctttgccaga
     3961 gagcgacgac ttccccttgc gttggtgatt gccggtcagg gcagccatcc gccatcgtcg
     4021 cgtagggtgt cacaccccag gaatcgcgtc actgaacaca gcagccggta ggacgaccat
     4081 gactgagttg gacaccatcg caaatccgtc cgatcccgcg gtgcagcgga tcatcgatgt
     4141 caccaagccg tcgcgatcca acataaagac aacgttgatc gaggacgtcg agcccctcat
     4201 gcacagcatc gcggccgggg tggagttcat cgaggtctac ggcagcgaca gcagtccttt
     4261 tccatctgag ttgctggatc tgtgcgggcg gcagaacata ccggtccgcc tcatcgactc
     4321 ctcgatcgtc aaccagttgt tcaaggggga gcggaaggcc aagacattcg gcatcgcccg
     4381 cgtccctcgc ccggccaggt tcggcgatat cgcgagccgg cgtggggacg tcgtcgttct
     4441 cgacggggtg aagatcgtcg ggaacatcgg cgcgatagta cgcacgtcgc tcgcgctcgg
     4501 agcgtcgggg atcatcctgg tcgacagtga catcaccagc atcgcggacc ggcgtctcca
     4561 aagggccagc cgaggttacg tcttctccct tcccgtcgtt ctctccggtc gcgaggaggc
     4621 catcgccttc attcgggaca gcggtatgca gctgatgacg ctcaaggcgg atggcgacat
     4681 ttccgtgaag gaactcgggg acaatccgga tcggctggcc ttgctgttcg gcagcgaaaa
     4741 gggtgggcct tccgacctgt tcgaggaggc gtcttccgcc tcggtttcca tccccatgat
     4801 gagccagacc gagtctctca acgtttccgt ttccctcgga atcgcgctgc acgagaggat
     4861 cgacaggaat ctcgcggcca accgataagc gcctctgttc ctcggacgct cggttgctcg
     4921 acctcgattc gtcagtgatg atcaacagga cccagaaatg gcacgagccc gggattggcc
     4981 tcccgggctc gccgcccccg aaccggcgca gcatgccgag tgaggacgtt tgctctgtga
     5041 cgagcgtacg agagaacagc gcgtcgacac caccgcgccc cgccggaccg aagtgccact
     5101 gcggcacccc gatcgtccac atccccggga agcgccccaa aatctactgc tcggacgcct
     5161 gccgaaagcg cgcaaagcgt ctcattgcgg accagtcacg cagcgtagca agcacgacta
     5221 cagcgggtaa tccggacgcg ggctttagta gtaagggaaa atctccgtcc actgcaggtc
     5281 agagcgctgt aactggaacg ggttctaccc caaacgaggg tgccgcgaca gccgggcggg
     5341 aggacccgcg cgacgagcgg ttcgcccggc gggaccggca ccagacggtg tcgctgaacg
     5401 aggcgttcaa gggctgcggg acccggctca ctgccgggac ggccgagctg atgtggaagc
     5461 cgggcgaggc gacgtggggc aacacgtgcc gctgcaacaa cgtccacacc tgcccgtggt
     5521 gcatgagccg catcctggcc gtacggggct cgaacgtgca gctcgccgcc gacggtctcg
     5581 cggacgccgg gtacgggctg caccttggca cgaacacgtt gcgccacttc gagcgcatgg
     5641 cgttcggcac cgtccgcaag ggaatgcggc acggcctggt cgcggtcttg cacgacggct
     5701 ggaagggcgc gtacggctcg tccggccgcc gctggcgcac gatgcgcgac gacttcggga
     5761 tcatcggcta cgagcgggcc ttcgaggaca ccttcggctg gggctccggc tggcacctgc
     5821 actggcacac gctctgggtc acccgcgagg tgctcggccc ggacgcccag gccgcgttcc
     5881 gcgacgcact cgccggagcc tgggccgccg gggtcgaatc cgcgggcggc tacaccgtca
     5941 gcgagacgtg cgaccggccc ggttgctcct gtgagggcaa gggccacggc accgacgtgc
     6001 gcccactcaa cggcgcggac gccgctgacg gcgatgcggg caagcaggcc cgctacctct
     6061 acaaggacgg cgacaagacc aagggcggcg tcgccaagat cggcctggag ctggccggac
     6121 agaacttcaa ggccggtcgc ggcgacgacc gcatgggacc gctcgacctg ggcgacgcgg
     6181 cggccgccga gctgcagcgg cttcgtcggc ccggcccgtt cgtcgagaag taccgcgagc
     6241 gcgagttcgg cgtcttccag gtccgcaagc actaccggtc gcagaacctg aaccggctga
     6301 tcaaggaact cggcatccag caggacgtcc gcaccgagga ggagatcacc gacgacaccg
     6361 agggcctggt cgccatcgcg gtcatcccgg cctacatctg gtaccgatac atcgcccgcg
     6421 tcgccggacg tcgcctcgac ctcatcaagg tcgccgagac gtacggccta ccgggcgtcc
     6481 ggcggctcgt cgagtcctgg ggcctggtgt ggggcaagga cgtcctggac ccgcccgccc
     6541 cggaagcgcc ggccgcgccg ggcgaccttg acgctgacca gatgaggttc gaggtcatgt
     6601 ccgaggagga agctgctttc cgcgaggcgc gccggaaggc caacgaggcc cgcacggagg
     6661 agctggccgc ttcgctcgac cgagtgcgtc agccgaagaa ggaggcgatc cgcccgacga
     6721 tcagccttcg gaagcgcctg aagccgaagc cggtcaccgt cgacgtcaag actcccccgc
     6781 ccggcgccgc gtcgcccgtg tgccgccgct gcaagggcaa gctcgccccg gtactccagc
     6841 cctggggccg gctccccgga gactgcctcc gcgtcgacac agcggtcgcg tagggccgct
     6901 gagggaccgc catgagaccg ccaccccgga catccgggtg gcggtctcat ggcggtcccg
     6961 gcgcgaaagc cgagggtctg cctgccgtga ggtggccggc ggacctgggt tactgctgga
     7021 atcccgcgcc cagtccgagc gtccgccggc gcttttcacc tggctccggg tgctcgtcga
     7081 tgagcacca
//
