LOCUS       pGLuc-Basic_2.        4958 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian promoter reporter vector encoding secreted Gaussia 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGLuc-Basic 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..68
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             76..630
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     polyA_signal    642..690
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      824..1252
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1266..1596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1663..2454
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2631..2764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2801..2817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2825..2841)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2849..2879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2894..2915)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3203..3791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3965..4822)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4823..4927)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcttgg aattctgcag atatcctcga gcccaagctt ggtaccgagc
       61 tcggatccag ccaccatggg agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc
      121 gaggccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc
      181 gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag
      241 gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc
      301 tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac
      361 acctacgaag gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtcgacatt
      421 cctgagattc ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat
      481 ctgtgtgtgg actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac
      541 ctgctcaaga agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag
      601 gtggacaaga tcaagggggc cggtggtgac taagcggccg caataaaata tctttatttt
      661 cattacatct gtgtgttggt tttttgtgtg tctagaaata attcttactg tcatgccaag
      721 taagatgctt ttctgtgctg caatagcagg catgctgggg atgcggtggg ctctatggct
      781 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
      841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      961 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
     1021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
     1201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     1261 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     1321 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     1381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     1441 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     1501 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     1561 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     1621 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     1681 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     1741 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     1801 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     1861 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     1921 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     1981 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     2041 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     2101 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     2161 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     2221 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     2281 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     2341 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     2401 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     2461 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     2521 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     2581 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     2641 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2701 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2761 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3001 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3361 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     3421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3601 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4921 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
