LOCUS       62056_11775        3162 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector pGLM171(AmpR-pUCori), complete sequence.
ACCESSION   MT891339
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan GB, Khammash M, 
            Siciliano V.
  TITLE     Characterization and mitigation of gene expression burden in 
            mammalian cells
  JOURNAL   Nat Commun 11 (1), 4641 (2020)
  PUBMED    32934213
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan G-B., Khammash M, 
            Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2020) Synthetic and Systems Biology for 
            Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e 
            Martteucci 53, Naples, Campania 80125, Italy
REFERENCE   3  (bases 1 to 3162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4641"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian
            Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples,
            Campania 80125, Italy"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..266
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     polyA_signal    283..507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(553..571)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    729..850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      940..1229
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     rep_origin      complement(1317..1905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2079..2936)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2937..3041)
                     /label=AmpR promoter
ORIGIN
        1 gtcttctcca tcggatcctg atcaggggag ctcacgggga cagccccccc ccaaagcccc
       61 cagggatgta attacgtccc tcccccgcta gggggcagca gcgaccgccc ggggctccgc
      121 tccggtccgg cgctcccccc gcatcccgag ccggcagcgt gcggggacag cccgggcacg
      181 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
      241 acacctgggg gatacgggga aaaccgcggc atatggcctc gactgtgcct tctagttgcc
      301 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      361 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      421 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
      481 atgctgggga tgcggtgggc tctatggctt ctgagggcta gtatgtacaa gtagtaatct
      541 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag tccgtcgacg
      601 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
      661 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      721 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      781 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      841 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      901 tgttaaatca gctcattttt taaccaatag gccgaaatcg gggagctcac ggggacagcc
      961 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgac
     1021 cgcccggggc tccgctccgg tccggcgctc cccccgcatc ccgagccggc agcgtgcggg
     1081 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
     1141 tctttgagcc tgcagacacc tgggggatac ggggaaaatg atcaagatct tctacactgg
     1201 gacgcaagga ttatcctcga ggcgcgcgaa gaatcagggg ataacgcagg aaagaacatg
     1261 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1741 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1981 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2041 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3061 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     3121 tctgctctga tgccgcatag ttaagccagt atctgctccc tg
//
