LOCUS       62056_11760        5588 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             89..1738
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1785..1906)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2100..2457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2488..3279
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3307..3355
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3682..4270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4473..5330)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5497..5588
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctagcctcga ggagctcacg cgtagatctg cagaagcttg
       61 gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc
      121 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
      181 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
      241 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
      301 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
      361 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
      421 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
      481 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
      541 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
      601 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
      661 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
      721 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
      781 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
      841 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
      901 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
      961 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
     1021 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
     1081 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
     1141 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
     1201 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg
     1261 cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct
     1321 catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca
     1381 cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc
     1441 agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg
     1501 cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa
     1561 aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa
     1621 gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga
     1681 cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta
     1741 ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     1801 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1861 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1921 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     1981 cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg
     2041 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc
     2101 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2161 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2281 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2341 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     2401 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     2461 ctcgattctt ctgacactag cgccaccatg atcgaacaag acggcctcca tgctggcagt
     2521 cccgcagctt gggtcgaacg cttgttcggg tacgactggg cccagcagac catcggatgt
     2581 agcgatgcgg ccgtgttccg tctaagcgct caaggccggc ccgtgctgtt cgtgaagacc
     2641 gacctgagcg gcgccctgaa cgagcttcaa gacgaggctg cccgcctgag ctggctggcc
     2701 accaccggcg taccctgcgc cgctgtgttg gatgttgtga ccgaagccgg ccgggactgg
     2761 ctgctgctgg gcgaggtccc tggccaggat ctgctgagca gccaccttgc ccccgctgag
     2821 aaggtttcta tcatggccga tgcaatgcgg cgcctgcaca ccctggaccc cgctacctgc
     2881 cccttcgacc accaggctaa gcatcggatc gagcgtgctc ggacccgcat ggaggccggc
     2941 ctggtggacc aggacgacct ggacgaggag catcagggcc tggcccccgc tgaactgttc
     3001 gcccgactga aagcccgcat gccggacggt gaggacctgg ttgtcacaca cggagatgcc
     3061 tgcctcccta acatcatggt cgagaatggc cgcttctccg gcttcatcga ctgcggtcgc
     3121 ctaggagttg ccgaccgcta ccaggacatc gccctggcca cccgcgacat cgctgaggag
     3181 cttggcggcg agtgggccga ccgcttctta gtcttgtacg gcatcgcagc tcccgacagc
     3241 cagcgcatcg ccttctaccg cttgctcgac gagttctttt aatgatctag aaccggtcat
     3301 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgttcga
     3361 actagatgct gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     3421 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     3481 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3541 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3601 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4021 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4081 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4141 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4201 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4261 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4321 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4381 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4441 cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc
     4501 tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact
     4561 acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt
     4621 tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt
     4681 ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta
     4741 agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta
     4801 tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc
     4861 acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc
     4921 agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt
     4981 accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt
     5041 tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc
     5101 gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa
     5161 gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt
     5221 tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa
     5281 aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt
     5341 tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt
     5401 aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca
     5461 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     5521 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     5581 atttctct
//
