LOCUS       62056_11755        6502 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1003..2652
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2699..2820)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3014..3371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3402..4193
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4221..4269
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4596..5184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5387..6244)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    6411..6502
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccc ctgagcaggc actgagtata agtcagacct gaatgtgcct
       61 ggaagagtgt cccacgcatt ccagcaggga agcagtttgt atgacaggtg tcccagtcca
      121 ggcggatacc aggtgctgcc agagtgtgga ggaggcaggc ggggacttag tctcctccct
      181 gggtttggac actggcatcc tgctttatgt gtgacaccac tgcacccctc tgagcctcgg
      241 tttccccatc tgtaaaatag aagcgatcta ccctcacagg tcagttgtag ggatgaacca
      301 tgaaaatact agagtctctg ttttttgaca ggaactcaaa aaacagatcc taaatgtaca
      361 tttaaagagg gtgtgaggag gcaagcagtc agcagaggat tccagcaggt gacattttag
      421 ggagctggag acagcagagc ctggggttgc taagttcctg atgttgccca ccaggctatt
      481 gctctgagca gcgctgcctc ccagctttct ggaaccttct gggacgcctg gggtgcatca
      541 agtcccaagg ggacagggag cagaaggggg ggctctggaa ggagcaaaat cacacccaga
      601 gcctgcagct tctcagattt ccttaaaggt tttgtgtgtg tgtgtgtgtg tgtgtgtgtg
      661 tgtatgtgtg tgtgtgtgtg tgtgtgtgtg tgttttctct aaaagtccta tggccagact
      721 ttgtttccca agggtcatat gactgctcct ctccacccca cactggcccg gggcgggctg
      781 ggcgcgggcc cctgcgggtg ttgcaacgcc cggccagaaa gtgggcatca gctgttccgc
      841 ctggcccacg tgacccgccg agcataaatg tgaccggccg cggctccggc agtcaacgcc
      901 tgcctcctct cgagcgtcct cagcgcagcc gccgcccgcg gagccagcac gaacgagaga
      961 tctgcagaag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac
     1021 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
     1081 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
     1141 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
     1201 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
     1261 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
     1321 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
     1381 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
     1441 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
     1501 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
     1561 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
     1621 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
     1681 ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc
     1741 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     1801 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     1861 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     1921 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     1981 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     2041 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     2101 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     2161 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     2221 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
     2281 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     2341 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     2401 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     2461 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     2521 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     2581 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     2641 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     2701 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2761 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2821 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2881 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt tgcgtattgg
     2941 gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     3001 ggttagctac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     3061 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3121 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3181 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3241 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3301 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3361 gcttttgcaa aaagctcgat tcttctgaca ctagcgccac catgatcgaa caagacggcc
     3421 tccatgctgg cagtcccgca gcttgggtcg aacgcttgtt cgggtacgac tgggcccagc
     3481 agaccatcgg atgtagcgat gcggccgtgt tccgtctaag cgctcaaggc cggcccgtgc
     3541 tgttcgtgaa gaccgacctg agcggcgccc tgaacgagct tcaagacgag gctgcccgcc
     3601 tgagctggct ggccaccacc ggcgtaccct gcgccgctgt gttggatgtt gtgaccgaag
     3661 ccggccggga ctggctgctg ctgggcgagg tccctggcca ggatctgctg agcagccacc
     3721 ttgcccccgc tgagaaggtt tctatcatgg ccgatgcaat gcggcgcctg cacaccctgg
     3781 accccgctac ctgccccttc gaccaccagg ctaagcatcg gatcgagcgt gctcggaccc
     3841 gcatggaggc cggcctggtg gaccaggacg acctggacga ggagcatcag ggcctggccc
     3901 ccgctgaact gttcgcccga ctgaaagccc gcatgccgga cggtgaggac ctggttgtca
     3961 cacacggaga tgcctgcctc cctaacatca tggtcgagaa tggccgcttc tccggcttca
     4021 tcgactgcgg tcgcctagga gttgccgacc gctaccagga catcgccctg gccacccgcg
     4081 acatcgctga ggagcttggc ggcgagtggg ccgaccgctt cttagtcttg tacggcatcg
     4141 cagctcccga cagccagcgc atcgccttct accgcttgct cgacgagttc ttttaatgat
     4201 ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     4261 ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc aacccagtca
     4321 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4381 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     4441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5341 acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca
     5401 gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg
     5461 tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc
     5521 cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg
     5581 ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc
     5641 gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta
     5701 ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc
     5761 ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc
     5821 ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac
     5881 tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact
     5941 caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta
     6001 tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt
     6061 cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca
     6121 ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa
     6181 aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc
     6241 tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc
     6301 aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct
     6361 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     6421 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     6481 gcaggtgcca gaacatttct ct
//
