LOCUS       pGL4.81[hRlucCP        5061 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding highly destabilized Renilla luciferase 
            for measuring the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.81[hRlucCP Neo]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1032
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             1039..1086
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
     CDS             1090..1209
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1258..1379)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1573..1930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1961..2752
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2780..2828
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3155..3743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3946..4803)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4908..4956
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4970..5061
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
     1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
     1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
     1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgatcgaac aagacggcct
     1981 ccatgctggc agtcccgcag cttgggtcga acgcttgttc gggtacgact gggcccagca
     2041 gaccatcgga tgtagcgatg cggccgtgtt ccgtctaagc gctcaaggcc ggcccgtgct
     2101 gttcgtgaag accgacctga gcggcgccct gaacgagctt caagacgagg ctgcccgcct
     2161 gagctggctg gccaccaccg gcgtaccctg cgccgctgtg ttggatgttg tgaccgaagc
     2221 cggccgggac tggctgctgc tgggcgaggt ccctggccag gatctgctga gcagccacct
     2281 tgcccccgct gagaaggttt ctatcatggc cgatgcaatg cggcgcctgc acaccctgga
     2341 ccccgctacc tgccccttcg accaccaggc taagcatcgg atcgagcgtg ctcggacccg
     2401 catggaggcc ggcctggtgg accaggacga cctggacgag gagcatcagg gcctggcccc
     2461 cgctgaactg ttcgcccgac tgaaagcccg catgccggac ggtgaggacc tggttgtcac
     2521 acacggagat gcctgcctcc ctaacatcat ggtcgagaat ggccgcttct ccggcttcat
     2581 cgactgcggt cgcctaggag ttgccgaccg ctaccaggac atcgccctgg ccacccgcga
     2641 catcgctgag gagcttggcg gcgagtgggc cgaccgcttc ttagtcttgt acggcatcgc
     2701 agctcccgac agccagcgca tcgccttcta ccgcttgctc gacgagttct tttaatgatc
     2761 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     2821 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag
     2881 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     2941 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     3001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3901 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     3961 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     4021 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     4081 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     4141 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     4201 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     4261 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     4321 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     4381 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     4441 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     4501 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     4561 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     4621 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     4681 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     4741 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     4801 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     4861 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
     4921 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     4981 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     5041 caggtgccag aacatttctc t
//
