LOCUS       pGL4.78[hRlucCP        5303 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding highly destabilized Renilla luciferase 
            for measuring the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.78[hRlucCP Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1032
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             1039..1086
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
     CDS             1090..1209
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1258..1379)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1573..1930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1961..2995
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3022..3070
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3397..3985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4188..5045)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5150..5198
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5212..5303
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
     1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
     1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
     1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgaagaagc ccgaactcac
     1981 cgctaccagc gttgaaaaat ttctcatcga gaagttcgac agtgtgagcg acctgatgca
     2041 gttgtcggag ggcgaagaga gccgagcctt cagcttcgat gtcggcggac gcggctatgt
     2101 actgcgggtg aatagctgcg ctgatggctt ctacaaagac cgctacgtgt accgccactt
     2161 cgccagcgct gcactaccca tccccgaagt gttggacatc ggcgagttca gcgagagcct
     2221 gacatactgc atcagtagac gcgcccaagg cgttactctc caagacctcc ccgaaacaga
     2281 gctgcctgct gtgttacagc ctgtcgccga agctatggat gctattgccg ccgccgacct
     2341 cagtcaaacc agcggcttcg gcccattcgg gccccaaggc atcggccagt acacaacctg
     2401 gcgggatttc atttgcgcca ttgctgatcc ccatgtctac cactggcaga ccgtgatgga
     2461 cgacaccgtg tccgccagcg tagctcaagc cctggacgaa ctgatgctgt gggccgaaga
     2521 ctgtcccgag gtgcgccacc tcgtccatgc cgacttcggc agcaacaacg tcctgaccga
     2581 caacggccgc atcaccgccg taatcgactg gtccgaagct atgttcgggg acagtcagta
     2641 cgaggtggcc aacatcttct tctggcggcc ctggctggct tgcatggagc agcagactcg
     2701 ctacttcgag cgccggcatc ccgagctggc cggcagccct cgtctgcgag cctacatgct
     2761 gcgcatcggc ctggatcagc tctaccagag cctcgtggac ggcaacttcg acgatgctgc
     2821 ctgggctcaa ggccgctgcg atgccatcgt ccgcagcggg gccggcaccg tcggtcgcac
     2881 acaaatcgct cgccggagcg cagccgtatg gaccgacggc tgcgtcgagg tgctggccga
     2941 cagcggcaac cgccggccca gtacacgacc gcgcgctaag gaggtaggtc gagtttaaac
     3001 tctagaaccg gtcatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     3061 tttttgtgtg ttcgaactag atgctgtcga ccgatgccct tgagagcctt caacccagtc
     3121 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     3181 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     3241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4141 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
     4201 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
     4261 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
     4321 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
     4381 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
     4441 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
     4501 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
     4561 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
     4621 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
     4681 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
     4741 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
     4801 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
     4861 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
     4921 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
     4981 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
     5041 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
     5101 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
     5161 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5221 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5281 tgcaggtgcc agaacatttc tct
//
