LOCUS       40924_22338        4237 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Co-transfection vector for expressing Renilla luciferase under 
            control of the medium-strength HSV thymidine kinase promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..779
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             815..1747
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(1791..1912)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2331..2919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3122..3979)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4084..4132
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4146..4237
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggct tccaaggtgt acgaccccga
      841 gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc aaatgaacgt
      901 gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg ccgtgatttt
      961 tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc acatcgagcc
     1021 cgtggctaga tgcatcatcc ctgatctgat cggaatgggt aagtccggca agagcgggaa
     1081 tggctcatat cgcctcctgg atcactacaa gtacctcacc gcttggttcg agctgctgaa
     1141 ccttccaaag aaaatcatct ttgtgggcca cgactggggg gcttgtctgg cctttcacta
     1201 ctcctacgag caccaagaca agatcaaggc catcgtccat gctgagagtg tcgtggacgt
     1261 gatcgagtcc tgggacgagt ggcctgacat cgaggaggat atcgccctga tcaagagcga
     1321 agagggcgag aaaatggtgc ttgagaataa cttcttcgtc gagaccatgc tcccaagcaa
     1381 gatcatgcgg aaactggagc ctgaggagtt cgctgcctac ctggagccat tcaaggagaa
     1441 gggcgaggtt agacggccta ccctctcctg gcctcgcgag atccctctcg ttaagggagg
     1501 caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc taccttcggg ccagcgacga
     1561 tctgcctaag atgttcatcg agtccgaccc tgggttcttt tccaacgcta ttgtcgaggg
     1621 agctaagaag ttccctaaca ccgagttcgt gaaggtgaag ggcctccact tcagccagga
     1681 ggacgctcca gatgaaatgg gtaagtacat caagagcttc gtggagcgcg tgctgaagaa
     1741 cgagcagtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     1801 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1861 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1921 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1981 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag
     2041 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     2101 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3061 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     3121 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     3181 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     3241 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     3301 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     3361 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     3421 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     3481 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     3541 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     3601 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     3661 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     3721 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     3781 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     3841 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     3901 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     3961 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
     4021 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
     4081 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     4141 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     4201 gtccccagtg caagtgcagg tgccagaaca tttctct
//
