LOCUS       Exported                3921 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.73[hRluc/SV40]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   vfsd
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    68..116
                     /note="synthetic polyadenylation signal"
     misc_feature    130..221
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        327..684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      535..670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             720..1655
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1696..1817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2236..2824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3024..3884)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggttactagt acgtctctca aggataagta agtaatatta aggtacggga ggtattggac
       61 aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
      121 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
      181 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tggcctaact ggccggtacc
      241 tgagctcgct agcctcgagg atatcagatc tgcgcagcac catggcctga aataacctct
      301 gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt
      361 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      421 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      481 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      661 tttggaggcc taggcttttg caaaaagctt ggcaatccgg tactgttggt aaagccacca
      721 tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt
      781 gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga
      841 agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga
      901 ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa
      961 tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc
     1021 tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact
     1081 ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg
     1141 tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg
     1201 aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct
     1261 tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg
     1321 cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc
     1381 gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca
     1441 acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt
     1501 tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg
     1561 tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga
     1621 gcttcgtgga gcgcgtgctg aagaacgagc agtaattcta gagtcggggc ggccggccgc
     1681 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1741 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1801 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1861 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     1921 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     1981 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     2041 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2161 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2641 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2881 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2941 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg
     3001 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg
     3061 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt
     3121 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg
     3181 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct
     3241 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt
     3301 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc
     3361 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga
     3421 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt
     3481 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc
     3541 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag
     3601 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca
     3661 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca
     3721 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct
     3781 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc
     3841 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa
     3901 tattattgaa gcatttatca g
//
