LOCUS       pGL4.71[hRlucP].        3653 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding destabilized Renilla luciferase for 
            measuring the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.71[hRlucP]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1032
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             1036..1155
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1207..1328)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1747..2335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2538..3395)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3500..3548
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3562..3653
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
     1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
     1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
     1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     1441 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     1501 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     1561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
     2521 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
     2581 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
     2641 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
     2701 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
     2761 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
     2821 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
     2881 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
     2941 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
     3001 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
     3061 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
     3121 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
     3181 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
     3241 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
     3301 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
     3361 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
     3421 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
     3481 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     3541 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     3601 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
