LOCUS       pGL4.70[hRluc].        3522 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a Renilla luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.70[hRluc]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1032
                     /label=hRluc
                     /note="Renilla luciferase"
     polyA_signal    complement(1076..1197)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1616..2204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2407..3264)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3369..3417
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3431..3522
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     1321 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     1381 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2041 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2341 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
     2401 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
     2461 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
     2521 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
     2581 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
     2641 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
     2701 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
     2761 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
     2821 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
     2881 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
     2941 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
     3001 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
     3061 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
     3121 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
     3181 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
     3241 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
     3301 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
     3361 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     3421 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     3481 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//
