LOCUS       pGL4.49[luc2P_TC        6124 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a TCF/LEF response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.49[luc2P TCF-LEF RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..404
                     /label=TCF/LEF transcription factors binding site
                     /bound_moiety="TCF/LEF transcription factors"
                     /note="TCF/LEF RE"
                     /note="TCF/LEF response element (8 copies)"
     promoter        449..480
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             513..2162
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2166..2285
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2337..2458)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2652..3009
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3040..4074
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4101..4149
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4476..5064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5267..6124)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctcagatca aagggtttaa
      301 gatcaaaggg cttaagatca aagggtataa gatcaaaggg cctaagatca aagggactaa
      361 gatcaaaggg tttaagatca aagggcttaa gatcaaaggg cctactcgag gatatcaaga
      421 tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag
      481 cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac attaagaagg
      541 gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca
      601 tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg
      661 acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct
      721 atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca
      781 tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca
      841 acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca
      901 agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca
      961 tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt
     1021 cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca
     1081 aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc
     1141 taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc
     1201 agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt
     1261 tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg
     1321 aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca
     1381 cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc
     1441 acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac
     1501 gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc
     1561 tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg
     1621 aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc
     1681 tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
     1741 ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg
     1801 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
     1861 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
     1921 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
     1981 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
     2041 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
     2101 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
     2161 tgaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga
     2221 gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca
     2281 acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag
     2341 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2401 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2461 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2521 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg cgtattgggc
     2581 gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     2641 ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2761 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2821 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2881 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2941 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3001 ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaagaagcc cgaactcacc
     3061 gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga cctgatgcag
     3121 ttgtcggagg gcgaagagag ccgagccttc agcttcgatg tcggcggacg cggctatgta
     3181 ctgcgggtga atagctgcgc tgatggcttc tacaaagacc gctacgtgta ccgccacttc
     3241 gccagcgctg cactacccat ccccgaagtg ttggacatcg gcgagttcag cgagagcctg
     3301 acatactgca tcagtagacg cgcccaaggc gttactctcc aagacctccc cgaaacagag
     3361 ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg ctattgccgc cgccgacctc
     3421 agtcaaacca gcggcttcgg cccattcggg ccccaaggca tcggccagta cacaacctgg
     3481 cgggatttca tttgcgccat tgctgatccc catgtctacc actggcagac cgtgatggac
     3541 gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg ggccgaagac
     3601 tgtcccgagg tgcgccacct cgtccatgcc gacttcggca gcaacaacgt cctgaccgac
     3661 aacggccgca tcaccgccgt aatcgactgg tccgaagcta tgttcgggga cagtcagtac
     3721 gaggtggcca acatcttctt ctggcggccc tggctggctt gcatggagca gcagactcgc
     3781 tacttcgagc gccggcatcc cgagctggcc ggcagccctc gtctgcgagc ctacatgctg
     3841 cgcatcggcc tggatcagct ctaccagagc ctcgtggacg gcaacttcga cgatgctgcc
     3901 tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt cggtcgcaca
     3961 caaatcgctc gccggagcgc agccgtatgg accgacggct gcgtcgaggt gctggccgac
     4021 agcggcaacc gccggcccag tacacgaccg cgcgctaagg aggtaggtcg agtttaaact
     4081 ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     4141 ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc aacccagtca
     4201 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4261 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     4321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5221 acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca
     5281 gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg
     5341 tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc
     5401 cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg
     5461 ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc
     5521 gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta
     5581 ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc
     5641 ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc
     5701 ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac
     5761 tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact
     5821 caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta
     5881 tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt
     5941 cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca
     6001 ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa
     6061 aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc
     6121 tcat
//
