LOCUS       pGL4.47[luc2P_SI        6129 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a sis-inducible element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.47[luc2P SIE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..409
                     /label=b
                     /bound_moiety="STAT3 homodimers and STAT3/STAT1
                     heterodimers"
                     /note="SIE"
                     /note="sis-inducible element (5 copies)"
     promoter        454..485
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             518..2167
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2171..2290
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2342..2463)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2657..3014
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3045..4079
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4106..4154
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4481..5069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5272..6129)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctcagcttc atttcccgta
      301 aatcgtcgaa gcttcatttc ccgtaaatcg tcgaagcttc atttcccgta aatcgtcgaa
      361 gcttcatttc ccgtaaatcg tcgaagcttc atttcccgta aatcgtcgac tcgaggatat
      421 caagatctgg cctcggcggc caagcttaga cactagaggg tatataatgg aagctcgact
      481 tccagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca aaaacattaa
      541 gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc agctgcacaa
      601 agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg cacatatcga
      661 ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag aagctatgaa
      721 gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata gcttgcagtt
      781 cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag ctaacgacat
      841 ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg tcgtattcgt
      901 gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa
      961 gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt acaccttcgt
     1021 gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg
     1081 ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc ccaagggcgt
     1141 agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg
     1201 caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc acggcttcgg
     1261 catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca tgtaccgctt
     1321 cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt
     1381 gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa
     1441 cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc
     1501 caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc
     1561 cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt
     1621 cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg
     1681 cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac
     1741 aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga
     1801 ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca
     1861 ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg
     1921 ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga
     1981 acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac
     2041 cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg
     2101 caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat
     2161 cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc
     2221 catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag
     2281 gatcaacgtc taaggccgcg actctagagt cggggcggcc ggccgcttcg agcagacatg
     2341 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     2401 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     2461 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     2521 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtttgcgtat
     2581 tgggcgctct tccgctgatc tgcgcagcac catggcctga aataacctct gaaagaggaa
     2641 cttggttagc taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     2701 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2761 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2821 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2881 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2941 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3001 taggcttttg caaaaagctc gattcttctg acactagcgc caccatgaag aagcccgaac
     3061 tcaccgctac cagcgttgaa aaatttctca tcgagaagtt cgacagtgtg agcgacctga
     3121 tgcagttgtc ggagggcgaa gagagccgag ccttcagctt cgatgtcggc ggacgcggct
     3181 atgtactgcg ggtgaatagc tgcgctgatg gcttctacaa agaccgctac gtgtaccgcc
     3241 acttcgccag cgctgcacta cccatccccg aagtgttgga catcggcgag ttcagcgaga
     3301 gcctgacata ctgcatcagt agacgcgccc aaggcgttac tctccaagac ctccccgaaa
     3361 cagagctgcc tgctgtgtta cagcctgtcg ccgaagctat ggatgctatt gccgccgccg
     3421 acctcagtca aaccagcggc ttcggcccat tcgggcccca aggcatcggc cagtacacaa
     3481 cctggcggga tttcatttgc gccattgctg atccccatgt ctaccactgg cagaccgtga
     3541 tggacgacac cgtgtccgcc agcgtagctc aagccctgga cgaactgatg ctgtgggccg
     3601 aagactgtcc cgaggtgcgc cacctcgtcc atgccgactt cggcagcaac aacgtcctga
     3661 ccgacaacgg ccgcatcacc gccgtaatcg actggtccga agctatgttc ggggacagtc
     3721 agtacgaggt ggccaacatc ttcttctggc ggccctggct ggcttgcatg gagcagcaga
     3781 ctcgctactt cgagcgccgg catcccgagc tggccggcag ccctcgtctg cgagcctaca
     3841 tgctgcgcat cggcctggat cagctctacc agagcctcgt ggacggcaac ttcgacgatg
     3901 ctgcctgggc tcaaggccgc tgcgatgcca tcgtccgcag cggggccggc accgtcggtc
     3961 gcacacaaat cgctcgccgg agcgcagccg tatggaccga cggctgcgtc gaggtgctgg
     4021 ccgacagcgg caaccgccgg cccagtacac gaccgcgcgc taaggaggta ggtcgagttt
     4081 aaactctaga accggtcatg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     4141 tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg cccttgagag ccttcaaccc
     4201 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     4261 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     4321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4921 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4981 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5041 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5101 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5161 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5221 agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt accagtgctt
     5281 gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag tggcctgact
     5341 ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca gcgcagcaat
     5401 gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc agccagcagg
     5461 gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt ctatgagctg
     5521 ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag ttgtggccat
     5581 tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca actctggttc
     5641 ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag tcagctcctt
     5701 agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc
     5761 agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga
     5821 gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct cttgcccggc
     5881 gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca tcatcgggaa
     5941 tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca gttcgatata
     6001 gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg tttcggggtg
     6061 tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg
     6121 gatgctcat
//
