LOCUS       pGL4.43[luc2P_XR        6067 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a xenobiotic response element for
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.43[luc2P XRE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..347
                     /label=aryl hydrocarbon receptor AhR binding site
                     /bound_moiety="aryl hydrocarbon receptor AhR"
                     /note="XRE"
                     /note="xenobiotic response element (3 copies)"
     promoter        392..423
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             456..2105
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2109..2228
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2280..2401)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2595..2952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2983..4017
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4044..4092
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4419..5007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5210..6067)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctctgagtt ctcacgctag
      301 cagattgagt tctcacgcta gcagattgag ttctcacgct agcagatctc gaggatatca
      361 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
      421 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
      481 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
      541 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
      601 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
      661 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
      721 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
      781 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
      841 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
      901 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
      961 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
     1021 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
     1081 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
     1141 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
     1201 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
     1261 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
     1321 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
     1381 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
     1441 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
     1501 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
     1561 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
     1621 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
     1681 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     1741 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
     1801 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
     1861 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
     1921 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
     1981 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
     2041 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
     2101 ccgtgaattc tcacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
     2161 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
     2221 tcaacgtcta aggccgcgac tctagagtcg gggcggccgg ccgcttcgag cagacatgat
     2281 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2341 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2401 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2461 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt ttgcgtattg
     2521 ggcgctcttc cgctgatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     2581 tggttagcta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2701 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2761 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2881 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2941 ggcttttgca aaaagctcga ttcttctgac actagcgcca ccatgaagaa gcccgaactc
     3001 accgctacca gcgttgaaaa atttctcatc gagaagttcg acagtgtgag cgacctgatg
     3061 cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg atgtcggcgg acgcggctat
     3121 gtactgcggg tgaatagctg cgctgatggc ttctacaaag accgctacgt gtaccgccac
     3181 ttcgccagcg ctgcactacc catccccgaa gtgttggaca tcggcgagtt cagcgagagc
     3241 ctgacatact gcatcagtag acgcgcccaa ggcgttactc tccaagacct ccccgaaaca
     3301 gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg atgctattgc cgccgccgac
     3361 ctcagtcaaa ccagcggctt cggcccattc gggccccaag gcatcggcca gtacacaacc
     3421 tggcgggatt tcatttgcgc cattgctgat ccccatgtct accactggca gaccgtgatg
     3481 gacgacaccg tgtccgccag cgtagctcaa gccctggacg aactgatgct gtgggccgaa
     3541 gactgtcccg aggtgcgcca cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc
     3601 gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag
     3661 tacgaggtgg ccaacatctt cttctggcgg ccctggctgg cttgcatgga gcagcagact
     3721 cgctacttcg agcgccggca tcccgagctg gccggcagcc ctcgtctgcg agcctacatg
     3781 ctgcgcatcg gcctggatca gctctaccag agcctcgtgg acggcaactt cgacgatgct
     3841 gcctgggctc aaggccgctg cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc
     3901 acacaaatcg ctcgccggag cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc
     3961 gacagcggca accgccggcc cagtacacga ccgcgcgcta aggaggtagg tcgagtttaa
     4021 actctagaac cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     4081 gttttttgtg tgttcgaact agatgctgtc gaccgatgcc cttgagagcc ttcaacccag
     4141 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     4201 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     4261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5161 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
     5221 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
     5281 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
     5341 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
     5401 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
     5461 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
     5521 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
     5581 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
     5641 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
     5701 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
     5761 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
     5821 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
     5881 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
     5941 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
     6001 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
     6061 tgctcat
//
