LOCUS       pGL4.42[luc2P_HR        6080 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a hypoxia response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.42[luc2P HRE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..360
                     /label=hypoxia-inducible factor HIF-1 binding site
                     /bound_moiety="hypoxia-inducible factor HIF-1"
                     /note="HRE"
                     /note="hypoxia response element (4 copies)"
     promoter        405..436
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             469..2118
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2122..2241
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2293..2414)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2608..2965
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2996..4030
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4057..4105
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4432..5020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5223..6080)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctcgtgact acgtgctgcc
      301 taggtgacta cgtgctgcct aggtgactac gtgctgccta ggtgactacg tgctgcctag
      361 ctcgaggata tcaagatctg gcctcggcgg ccaagcttag acactagagg gtatataatg
      421 gaagctcgac ttccagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc
      481 aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag
      541 cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac
      601 gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca
      661 gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat
      721 agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca
      781 gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc
      841 gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg
      901 atcatacaaa agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg
      961 tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag
     1021 agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg
     1081 cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac
     1141 cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac
     1201 cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc
     1261 atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct
     1321 gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac
     1381 gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt
     1441 gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa
     1501 acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag
     1561 gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg
     1621 aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac
     1681 cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc
     1741 tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac
     1801 aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc
     1861 ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc
     1921 gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc
     1981 caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa
     2041 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag
     2101 ggcggcaaga tcgccgtgaa ttctcacggc ttccctcccg aggtggagga gcaggccgcc
     2161 ggcaccctgc ccatgagctg cgcccaggag agcggcatgg atagacaccc tgctgcttgc
     2221 gccagcgcca ggatcaacgt ctaaggccgc gactctagag tcggggcggc cggccgcttc
     2281 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2341 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2401 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2461 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2521 cgtttgcgta ttgggcgctc ttccgctgat ctgcgcagca ccatggcctg aaataacctc
     2581 tgaaagagga acttggttag ctaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     2641 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2701 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2761 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2821 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2881 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     2941 ttttggaggc ctaggctttt gcaaaaagct cgattcttct gacactagcg ccaccatgaa
     3001 gaagcccgaa ctcaccgcta ccagcgttga aaaatttctc atcgagaagt tcgacagtgt
     3061 gagcgacctg atgcagttgt cggagggcga agagagccga gccttcagct tcgatgtcgg
     3121 cggacgcggc tatgtactgc gggtgaatag ctgcgctgat ggcttctaca aagaccgcta
     3181 cgtgtaccgc cacttcgcca gcgctgcact acccatcccc gaagtgttgg acatcggcga
     3241 gttcagcgag agcctgacat actgcatcag tagacgcgcc caaggcgtta ctctccaaga
     3301 cctccccgaa acagagctgc ctgctgtgtt acagcctgtc gccgaagcta tggatgctat
     3361 tgccgccgcc gacctcagtc aaaccagcgg cttcggccca ttcgggcccc aaggcatcgg
     3421 ccagtacaca acctggcggg atttcatttg cgccattgct gatccccatg tctaccactg
     3481 gcagaccgtg atggacgaca ccgtgtccgc cagcgtagct caagccctgg acgaactgat
     3541 gctgtgggcc gaagactgtc ccgaggtgcg ccacctcgtc catgccgact tcggcagcaa
     3601 caacgtcctg accgacaacg gccgcatcac cgccgtaatc gactggtccg aagctatgtt
     3661 cggggacagt cagtacgagg tggccaacat cttcttctgg cggccctggc tggcttgcat
     3721 ggagcagcag actcgctact tcgagcgccg gcatcccgag ctggccggca gccctcgtct
     3781 gcgagcctac atgctgcgca tcggcctgga tcagctctac cagagcctcg tggacggcaa
     3841 cttcgacgat gctgcctggg ctcaaggccg ctgcgatgcc atcgtccgca gcggggccgg
     3901 caccgtcggt cgcacacaaa tcgctcgccg gagcgcagcc gtatggaccg acggctgcgt
     3961 cgaggtgctg gccgacagcg gcaaccgccg gcccagtaca cgaccgcgcg ctaaggaggt
     4021 aggtcgagtt taaactctag aaccggtcat ggccgcaata aaatatcttt attttcatta
     4081 catctgtgtg ttggtttttt gtgtgttcga actagatgct gtcgaccgat gcccttgaga
     4141 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     4201 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     4261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4861 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4981 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5041 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5161 aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt
     5221 taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata
     5281 gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc
     5341 agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac
     5401 cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag
     5461 tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga
     5521 gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc
     5581 aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca
     5641 gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc
     5701 atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc
     5761 gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc
     5821 tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc
     5881 atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc
     5941 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc
     6001 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca
     6061 cgaaaatgtt ggatgctcat
//
