LOCUS       pGL4.40[luc2P_MR        6079 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a metal response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.40[luc2P MRE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..359
                     /label=metal response element-binding transcription
                     fa
                     /bound_moiety="metal response element-binding transcription
                     factor-1 (MTF-1)"
                     /note="MRE"
                     /note="metal response element (5 copies)"
     promoter        404..435
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             468..2117
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2121..2240
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2292..2413)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2607..2964
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2995..4029
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4056..4104
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4431..5019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5222..6079)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctcgtctgc acacggcccg
      301 tctgcacacg gcccgtctgc acacggcccg tctgcacacg gcccgtctgc acacggcccc
      361 tcgaggatat caagatctgg cctcggcggc caagcttaga cactagaggg tatataatgg
      421 aagctcgact tccagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
      481 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
      541 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
      601 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
      661 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
      721 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      781 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
      841 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
      901 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
      961 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     1021 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     1081 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     1141 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
     1201 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     1261 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     1321 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     1381 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     1441 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     1501 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     1561 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1621 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1681 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     1741 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1801 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     1861 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     1921 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     1981 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     2041 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     2101 gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg
     2161 gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg
     2221 ccagcgccag gatcaacgtc taaggccgcg actctagagt cggggcggcc ggccgcttcg
     2281 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2341 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2401 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2461 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     2521 gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga aataacctct
     2581 gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     2641 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2701 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2761 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     2821 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     2881 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     2941 tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc caccatgaag
     3001 aagcccgaac tcaccgctac cagcgttgaa aaatttctca tcgagaagtt cgacagtgtg
     3061 agcgacctga tgcagttgtc ggagggcgaa gagagccgag ccttcagctt cgatgtcggc
     3121 ggacgcggct atgtactgcg ggtgaatagc tgcgctgatg gcttctacaa agaccgctac
     3181 gtgtaccgcc acttcgccag cgctgcacta cccatccccg aagtgttgga catcggcgag
     3241 ttcagcgaga gcctgacata ctgcatcagt agacgcgccc aaggcgttac tctccaagac
     3301 ctccccgaaa cagagctgcc tgctgtgtta cagcctgtcg ccgaagctat ggatgctatt
     3361 gccgccgccg acctcagtca aaccagcggc ttcggcccat tcgggcccca aggcatcggc
     3421 cagtacacaa cctggcggga tttcatttgc gccattgctg atccccatgt ctaccactgg
     3481 cagaccgtga tggacgacac cgtgtccgcc agcgtagctc aagccctgga cgaactgatg
     3541 ctgtgggccg aagactgtcc cgaggtgcgc cacctcgtcc atgccgactt cggcagcaac
     3601 aacgtcctga ccgacaacgg ccgcatcacc gccgtaatcg actggtccga agctatgttc
     3661 ggggacagtc agtacgaggt ggccaacatc ttcttctggc ggccctggct ggcttgcatg
     3721 gagcagcaga ctcgctactt cgagcgccgg catcccgagc tggccggcag ccctcgtctg
     3781 cgagcctaca tgctgcgcat cggcctggat cagctctacc agagcctcgt ggacggcaac
     3841 ttcgacgatg ctgcctgggc tcaaggccgc tgcgatgcca tcgtccgcag cggggccggc
     3901 accgtcggtc gcacacaaat cgctcgccgg agcgcagccg tatggaccga cggctgcgtc
     3961 gaggtgctgg ccgacagcgg caaccgccgg cccagtacac gaccgcgcgc taaggaggta
     4021 ggtcgagttt aaactctaga accggtcatg gccgcaataa aatatcttta ttttcattac
     4081 atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg cccttgagag
     4141 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     4201 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     4261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4861 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5161 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     5221 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     5281 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     5341 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     5401 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     5461 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     5521 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     5581 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     5641 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     5701 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     5761 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     5821 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     5881 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     5941 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     6001 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     6061 gaaaatgttg gatgctcat
//
