LOCUS       pGL4.39[luc2P_AT        6139 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and an ATF6 response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.39[luc2P ATF6 RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..419
                     /label=ATF6 binding site
                     /bound_moiety="ATF6"
                     /note="ATF6 RE"
                     /note="ATF6 response element (5 copies)"
     promoter        464..495
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             528..2177
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2181..2300
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2352..2473)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2667..3024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3055..4089
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4116..4164
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4491..5079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5282..6139)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctcatcgag acaggtgctg
      301 acgtggcatt catcgagaca ggtgctgacg tggcattcat cgagacaggt gctgacgtgg
      361 cattcatcga gacaggtgct gacgtggcat tcatcgagac aggtgctgac gtggcattcc
      421 tcgaggatat caagatctgg cctcggcggc caagcttaga cactagaggg tatataatgg
      481 aagctcgact tccagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
      541 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
      601 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
      661 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
      721 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
      781 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      841 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
      901 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
      961 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
     1021 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     1081 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     1141 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     1201 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
     1261 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     1321 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     1381 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     1441 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     1501 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     1561 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     1621 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1681 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1741 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     1801 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1861 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     1921 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     1981 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     2041 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     2101 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     2161 gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg
     2221 gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg
     2281 ccagcgccag gatcaacgtc taaggccgcg actctagagt cggggcggcc ggccgcttcg
     2341 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2401 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2461 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2521 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     2581 gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga aataacctct
     2641 gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     2701 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2761 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2821 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     2881 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     2941 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     3001 tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc caccatgaag
     3061 aagcccgaac tcaccgctac cagcgttgaa aaatttctca tcgagaagtt cgacagtgtg
     3121 agcgacctga tgcagttgtc ggagggcgaa gagagccgag ccttcagctt cgatgtcggc
     3181 ggacgcggct atgtactgcg ggtgaatagc tgcgctgatg gcttctacaa agaccgctac
     3241 gtgtaccgcc acttcgccag cgctgcacta cccatccccg aagtgttgga catcggcgag
     3301 ttcagcgaga gcctgacata ctgcatcagt agacgcgccc aaggcgttac tctccaagac
     3361 ctccccgaaa cagagctgcc tgctgtgtta cagcctgtcg ccgaagctat ggatgctatt
     3421 gccgccgccg acctcagtca aaccagcggc ttcggcccat tcgggcccca aggcatcggc
     3481 cagtacacaa cctggcggga tttcatttgc gccattgctg atccccatgt ctaccactgg
     3541 cagaccgtga tggacgacac cgtgtccgcc agcgtagctc aagccctgga cgaactgatg
     3601 ctgtgggccg aagactgtcc cgaggtgcgc cacctcgtcc atgccgactt cggcagcaac
     3661 aacgtcctga ccgacaacgg ccgcatcacc gccgtaatcg actggtccga agctatgttc
     3721 ggggacagtc agtacgaggt ggccaacatc ttcttctggc ggccctggct ggcttgcatg
     3781 gagcagcaga ctcgctactt cgagcgccgg catcccgagc tggccggcag ccctcgtctg
     3841 cgagcctaca tgctgcgcat cggcctggat cagctctacc agagcctcgt ggacggcaac
     3901 ttcgacgatg ctgcctgggc tcaaggccgc tgcgatgcca tcgtccgcag cggggccggc
     3961 accgtcggtc gcacacaaat cgctcgccgg agcgcagccg tatggaccga cggctgcgtc
     4021 gaggtgctgg ccgacagcgg caaccgccgg cccagtacac gaccgcgcgc taaggaggta
     4081 ggtcgagttt aaactctaga accggtcatg gccgcaataa aatatcttta ttttcattac
     4141 atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg cccttgagag
     4201 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     4261 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     4321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4921 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5221 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     5281 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     5341 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     5401 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     5461 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     5521 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     5581 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     5641 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     5701 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     5761 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     5821 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     5881 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     5941 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     6001 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     6061 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     6121 gaaaatgttg gatgctcat
//
