LOCUS       pGL4.38[luc2P_p5        6062 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a p53 response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.38[luc2P p53 RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..339
                     /label=p53 binding site
                     /bound_moiety="p53"
                     /note="p53 RE"
                     /note="p53 response element created by fusing two human p53
                     binding sites"
     promoter        387..418
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             451..2100
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2104..2223
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2275..2396)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2590..2947
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2978..4012
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4039..4087
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4414..5002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5205..6062)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctctacaga acatgtctaa
      301 gcatgctgtg ccttgcctgg acttgcctgg ccttgccttg ggctcgagga tatcaagatc
      361 tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct
      421 tggcaatccg gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc
      481 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
      541 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
      601 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
      661 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
      721 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
      781 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
      841 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
      901 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
      961 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     1021 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     1081 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     1141 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     1201 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     1261 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     1321 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
     1381 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     1441 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     1501 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     1561 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     1621 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     1681 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     1741 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     1801 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     1861 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     1921 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     1981 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     2041 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     2101 aattctcacg gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc
     2161 tgcgcccagg agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac
     2221 gtctaaggcc gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat
     2281 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2341 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2401 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2461 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc
     2521 tcttccgctg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     2581 agctaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     2641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2701 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2761 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     2821 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2881 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     2941 ttgcaaaaag ctcgattctt ctgacactag cgccaccatg aagaagcccg aactcaccgc
     3001 taccagcgtt gaaaaatttc tcatcgagaa gttcgacagt gtgagcgacc tgatgcagtt
     3061 gtcggagggc gaagagagcc gagccttcag cttcgatgtc ggcggacgcg gctatgtact
     3121 gcgggtgaat agctgcgctg atggcttcta caaagaccgc tacgtgtacc gccacttcgc
     3181 cagcgctgca ctacccatcc ccgaagtgtt ggacatcggc gagttcagcg agagcctgac
     3241 atactgcatc agtagacgcg cccaaggcgt tactctccaa gacctccccg aaacagagct
     3301 gcctgctgtg ttacagcctg tcgccgaagc tatggatgct attgccgccg ccgacctcag
     3361 tcaaaccagc ggcttcggcc cattcgggcc ccaaggcatc ggccagtaca caacctggcg
     3421 ggatttcatt tgcgccattg ctgatcccca tgtctaccac tggcagaccg tgatggacga
     3481 caccgtgtcc gccagcgtag ctcaagccct ggacgaactg atgctgtggg ccgaagactg
     3541 tcccgaggtg cgccacctcg tccatgccga cttcggcagc aacaacgtcc tgaccgacaa
     3601 cggccgcatc accgccgtaa tcgactggtc cgaagctatg ttcggggaca gtcagtacga
     3661 ggtggccaac atcttcttct ggcggccctg gctggcttgc atggagcagc agactcgcta
     3721 cttcgagcgc cggcatcccg agctggccgg cagccctcgt ctgcgagcct acatgctgcg
     3781 catcggcctg gatcagctct accagagcct cgtggacggc aacttcgacg atgctgcctg
     3841 ggctcaaggc cgctgcgatg ccatcgtccg cagcggggcc ggcaccgtcg gtcgcacaca
     3901 aatcgctcgc cggagcgcag ccgtatggac cgacggctgc gtcgaggtgc tggccgacag
     3961 cggcaaccgc cggcccagta cacgaccgcg cgctaaggag gtaggtcgag tttaaactct
     4021 agaaccggtc atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4081 ttgtgtgttc gaactagatg ctgtcgaccg atgcccttga gagccttcaa cccagtcagc
     4141 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     4201 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
     4261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5161 ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt
     5221 gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc
     5281 gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg
     5341 cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc
     5401 gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt
     5461 gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact
     5521 ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg
     5581 tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct
     5641 ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta
     5701 cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca
     5761 accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata
     5821 cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct
     5881 tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact
     5941 cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa
     6001 acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc
     6061 at
//
