LOCUS       62056_11735        6086 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        411..442
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             475..2124
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2128..2247
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(2299..2420)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2614..2971
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3002..4036
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     rep_origin      complement(4438..5026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5229..6086)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctctgagca agggcgagga
      301 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      361 gttcagctcg aggatatcaa gatctggcct cggcggccaa gcttagacac tagagggtat
      421 ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc caccatggaa
      481 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
      541 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
      601 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
      661 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
      721 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
      781 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
      841 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
      901 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
      961 agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg
     1021 cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag tggcagtacc
     1081 ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc
     1141 cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag cgtggtgcca
     1201 tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc
     1261 gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt
     1321 caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac
     1381 aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag
     1441 gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg
     1501 acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta
     1561 ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg
     1621 ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt
     1681 aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac
     1741 atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc
     1801 aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc
     1861 aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc
     1921 gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg
     1981 gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg
     2041 cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc
     2101 aagaagggcg gcaagatcgc cgtgaattct cacggcttcc ctcccgaggt ggaggagcag
     2161 gccgccggca ccctgcccat gagctgcgcc caggagagcg gcatggatag acaccctgct
     2221 gcttgcgcca gcgccaggat caacgtctaa ggccgcgact ctagagtcgg ggcggccggc
     2281 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2341 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2401 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2461 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     2521 aggatccgtt tgcgtattgg gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat
     2581 aacctctgaa agaggaactt ggttagctac cttctgaggc ggaaagaacc agctgtggaa
     2641 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2701 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2761 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2881 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     2941 aggctttttt ggaggcctag gcttttgcaa aaagctcgat tcttctgaca ctagcgccac
     3001 catgaagaag cccgaactca ccgctaccag cgttgaaaaa tttctcatcg agaagttcga
     3061 cagtgtgagc gacctgatgc agttgtcgga gggcgaagag agccgagcct tcagcttcga
     3121 tgtcggcgga cgcggctatg tactgcgggt gaatagctgc gctgatggct tctacaaaga
     3181 ccgctacgtg taccgccact tcgccagcgc tgcactaccc atccccgaag tgttggacat
     3241 cggcgagttc agcgagagcc tgacatactg catcagtaga cgcgcccaag gcgttactct
     3301 ccaagacctc cccgaaacag agctgcctgc tgtgttacag cctgtcgccg aagctatgga
     3361 tgctattgcc gccgccgacc tcagtcaaac cagcggcttc ggcccattcg ggccccaagg
     3421 catcggccag tacacaacct ggcgggattt catttgcgcc attgctgatc cccatgtcta
     3481 ccactggcag accgtgatgg acgacaccgt gtccgccagc gtagctcaag ccctggacga
     3541 actgatgctg tgggccgaag actgtcccga ggtgcgccac ctcgtccatg ccgacttcgg
     3601 cagcaacaac gtcctgaccg acaacggccg catcaccgcc gtaatcgact ggtccgaagc
     3661 tatgttcggg gacagtcagt acgaggtggc caacatcttc ttctggcggc cctggctggc
     3721 ttgcatggag cagcagactc gctacttcga gcgccggcat cccgagctgg ccggcagccc
     3781 tcgtctgcga gcctacatgc tgcgcatcgg cctggatcag ctctaccaga gcctcgtgga
     3841 cggcaacttc gacgatgctg cctgggctca aggccgctgc gatgccatcg tccgcagcgg
     3901 ggccggcacc gtcggtcgca cacaaatcgc tcgccggagc gcagccgtat ggaccgacgg
     3961 ctgcgtcgag gtgctggccg acagcggcaa ccgccggccc agtacacgac cgcgcgctaa
     4021 ggaggtaggt cgagtttaaa ctctagaacc ggtcatggcc gcaataaaat atctttattt
     4081 tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta gatgctgtcg accgatgccc
     4141 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     4201 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     4261 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4321 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4381 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4441 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4501 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4561 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4621 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4681 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4741 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4801 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4861 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4921 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4981 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5041 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5101 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5161 aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc
     5221 agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg
     5281 tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca
     5341 ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca
     5401 atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc
     5461 atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc
     5521 cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct
     5581 tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga
     5641 aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg
     5701 tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc
     5761 ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca
     5821 agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa
     5881 gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg
     5941 agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc
     6001 accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt
     6061 gcgacacgaa aatgttggat gctcat
//
