LOCUS       pGL4.36[luc2P_MM        7246 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with an MMTV LTR for studying cell signaling using 
            destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.36[luc2P MMTV Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..1328
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     CDS             1377..3026
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             3030..3149
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(3201..3322)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3516..3873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3904..4938
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4965..5013
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(5340..5928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6131..6988)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    7093..7141
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    7155..7246
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccgcagaaa tggttgaact cccgagagtg tcctacacct aggggagaag
       61 cagccaaggg gttgtttccc accaaggacg acccgtctgc gcacaaacgg atgagcccat
      121 cagacaaaga catattcatt ctctgctgca aacttggcat agctctgctt tgcctggggc
      181 tattggggga agttgcggtt cgtgctcgca gggctctcac ccttgactct ttcaataata
      241 actcttctgt gcaagattac aatctaaaca attcggagaa ctcgaccttc ctcctgaggc
      301 aaggaccaca gccaacttcc tcttacaagc cgcatcgatt ttgtccttca gaaatagaaa
      361 taagaatgct tgctaaaaat tatattttta ccaataagac caatccaata ggtagattat
      421 tagttactat gttaagaaat gaatcattat cttttagtac tatttttact caaattcaga
      481 agttagaaat gggaatagaa aatagaaaga gacgctcaac ctcaattgaa gaacaggtgc
      541 aaggactatt gaccacaggc ctagaagtaa aaaagggaaa aaagagtgtt tttgtcaaaa
      601 taggagacag gtggtggcaa ccagggactt ataggggacc ttacatctac agaccaacag
      661 atgccccctt accatataca ggaagatatg acttaaattg ggataggtgg gttacagtca
      721 atggctataa agtgttatat agatccctcc cctttcgtga aagactcgcc agagctagac
      781 ctccttggtg tatgttgtct caagaaaaga aagacgacat gaaacaacag gtacatgatt
      841 atatttatct aggaacagga atgcactttt ggggaaagat tttccatacc aaggagggga
      901 cagtggctgg actaatagaa cattattctg caaaaactta tggcatgagt tattatgatt
      961 agccttgatt tgcccaacct tgcggttccc aaggcttaag taagtttttg gttacaaact
     1021 gttcttaaaa caaggatgtg agacaagtgg tttcctgact tggtttggta tcaaaggttc
     1081 tgatctgagc tctgagtgtt ctattttcct atgttctttt ggaatttatc caaatcttat
     1141 gtaaatgctt atgtaaacca agatataaaa gagtgctgat tttttgagta aacttgcaac
     1201 agtcctaaca ttcacctctt gtgtgtttgt gtctgttcgc catcccgtct ccgctcgtca
     1261 cttatccttc actttccaga gggtcccccc gcagaccccg gcgaccctca ggtcggccga
     1321 ctgcggcagg cctcggcggc caagcttggc aatccggtac tgttggtaaa gccaccatgg
     1381 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
     1441 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
     1501 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
     1561 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
     1621 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
     1681 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
     1741 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     1801 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     1861 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     1921 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     1981 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     2041 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     2101 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     2161 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     2221 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     2281 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     2341 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     2401 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     2461 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     2521 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     2581 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
     2641 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     2701 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     2761 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     2821 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     2881 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     2941 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     3001 ccaagaaggg cggcaagatc gccgtgaatt ctcacggctt ccctcccgag gtggaggagc
     3061 aggccgccgg caccctgccc atgagctgcg cccaggagag cggcatggat agacaccctg
     3121 ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga ctctagagtc ggggcggccg
     3181 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3241 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3301 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3361 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     3421 taaggatccg tttgcgtatt gggcgctctt ccgctgatct gcgcagcacc atggcctgaa
     3481 ataacctctg aaagaggaac ttggttagct accttctgag gcggaaagaa ccagctgtgg
     3541 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3601 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3661 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3721 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3781 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     3841 ggaggctttt ttggaggcct aggcttttgc aaaaagctcg attcttctga cactagcgcc
     3901 accatgaaga agcccgaact caccgctacc agcgttgaaa aatttctcat cgagaagttc
     3961 gacagtgtga gcgacctgat gcagttgtcg gagggcgaag agagccgagc cttcagcttc
     4021 gatgtcggcg gacgcggcta tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa
     4081 gaccgctacg tgtaccgcca cttcgccagc gctgcactac ccatccccga agtgttggac
     4141 atcggcgagt tcagcgagag cctgacatac tgcatcagta gacgcgccca aggcgttact
     4201 ctccaagacc tccccgaaac agagctgcct gctgtgttac agcctgtcgc cgaagctatg
     4261 gatgctattg ccgccgccga cctcagtcaa accagcggct tcggcccatt cgggccccaa
     4321 ggcatcggcc agtacacaac ctggcgggat ttcatttgcg ccattgctga tccccatgtc
     4381 taccactggc agaccgtgat ggacgacacc gtgtccgcca gcgtagctca agccctggac
     4441 gaactgatgc tgtgggccga agactgtccc gaggtgcgcc acctcgtcca tgccgacttc
     4501 ggcagcaaca acgtcctgac cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa
     4561 gctatgttcg gggacagtca gtacgaggtg gccaacatct tcttctggcg gccctggctg
     4621 gcttgcatgg agcagcagac tcgctacttc gagcgccggc atcccgagct ggccggcagc
     4681 cctcgtctgc gagcctacat gctgcgcatc ggcctggatc agctctacca gagcctcgtg
     4741 gacggcaact tcgacgatgc tgcctgggct caaggccgct gcgatgccat cgtccgcagc
     4801 ggggccggca ccgtcggtcg cacacaaatc gctcgccgga gcgcagccgt atggaccgac
     4861 ggctgcgtcg aggtgctggc cgacagcggc aaccgccggc ccagtacacg accgcgcgct
     4921 aaggaggtag gtcgagttta aactctagaa ccggtcatgg ccgcaataaa atatctttat
     4981 tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc
     5041 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     5101 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     5161 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6061 tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact
     6121 gcagtggtta ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt
     6181 cgtccatagt ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat
     6241 caggccccag cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag
     6301 caatgaacca gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct
     6361 ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt
     6421 tccgaagagt tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg
     6481 cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa
     6541 gaaatgcagt cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt
     6601 tgtcgctcat ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat
     6661 gcttttccgt gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac
     6721 caagctgctc ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga
     6781 aagtgctcat catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat
     6841 tgagatccag ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt
     6901 tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga
     6961 gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt
     7021 atcagggtta ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat
     7081 tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     7141 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     7201 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct
//
