LOCUS       pGL4.35[luc2P_9X        6184 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a promoter and an extended Gal4 upstream activating 
            sequence for studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.35[luc2P 9XGAL4UAS Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..130
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        222..262
                     /note="Ad promoter"
                     /note="adenovirus major late promoter"
     CDS             315..1964
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1968..2087
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2139..2260)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2454..2811
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2842..3876
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3903..3951
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4278..4866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5069..5926)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    6031..6079
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6093..6184
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
       61 cctccgactc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
      181 ctccgaggaa ttccggagta ctgtcctccg aagacgctag cggggggcta taaaaggggg
      241 tgggggcgtt cgtcctcact ctagatctgc gatctaagta agcttggcaa tccggtactg
      301 ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
      361 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
      421 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
      481 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
      541 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
      601 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
      661 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
      721 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
      781 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
      841 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
      901 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
      961 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     1021 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     1081 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     1141 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     1201 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     1261 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     1321 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     1381 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     1441 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     1501 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     1561 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     1621 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     1681 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     1741 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     1801 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     1861 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     1921 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgaattct cacggcttcc
     1981 ctcccgaggt ggaggagcag gccgccggca ccctgcccat gagctgcgcc caggagagcg
     2041 gcatggatag acaccctgct gcttgcgcca gcgccaggat caacgtctaa ggccgcgact
     2101 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     2161 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2221 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2281 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     2341 caaatgtggt aaaatcgata aggatccgtt tgcgtattgg gcgctcttcc gctgatctgc
     2401 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttagctac cttctgaggc
     2461 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2641 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2701 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     2761 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgat
     2821 tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag cgttgaaaaa
     2881 tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga gggcgaagag
     2941 agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt gaatagctgc
     3001 gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc tgcactaccc
     3061 atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg catcagtaga
     3121 cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc tgtgttacag
     3181 cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac cagcggcttc
     3241 ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt catttgcgcc
     3301 attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt gtccgccagc
     3361 gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga ggtgcgccac
     3421 ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg catcaccgcc
     3481 gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc caacatcttc
     3541 ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga gcgccggcat
     3601 cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg cctggatcag
     3661 ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca aggccgctgc
     3721 gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc tcgccggagc
     3781 gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa ccgccggccc
     3841 agtacacgac cgcgcgctaa ggaggtaggt cgagtttaaa ctctagaacc ggtcatggcc
     3901 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta
     3961 gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     4021 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     4081 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     5041 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     5101 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     5161 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     5221 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     5281 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     5341 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     5401 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     5461 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     5521 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     5581 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     5641 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     5701 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     5761 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     5821 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     5881 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     5941 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
     6001 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
     6061 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     6121 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     6181 ctct
//
